Abstract：
:The de novo synthesis of triglyceride (TG) fatty acids (FA) and glycerol can be measured with stable isotope tracers. However, these methods typically do not inform the contribution of a given substrate to specific pathways on these synthetic processes. We integrated deuterated water (2H2O) measurement of de novo lipogenesis (DNL) and glycerol-3-phosphate (GLY) synthesis from all substrates with a 13C nuclear magnetic resonance (NMR) method that quantifies TG FA and glycerol enrichment from a specific [U-13C]precursor. This allowed the [U-13C]precursor contribution to DNL and GLY to be estimated. We applied this method in mice to determine the contributions of fructose and glucose supplemented in the drinking water to DNL and GLY in liver, mesenteric adipose tissue (MAT) and subcutaneous adipose tissue (SCAT). In liver, fructose contributed significantly more to DNL of saturated fatty acids (SFA) and oleate as well as to GLY compared to glucose. Moreover, its contribution to SFA synthesis was significantly higher compared to that of oleate. MAT and SCAT had lower fractional rates of total DNL and GLY compared to liver and glucose was utilized more predominantly than fructose for TG synthesis in these tissues. This novel 2H2O/13C integrated method revealed for the first time, tissue specific selection of substrates for DNL, particularly fructose in regard to glucose in liver. Also, this approach was able to resolve the distribution of specific FAs into the TG sn2 and sn1,3 sites. This stable isotope integrated approach yielded information so far uncovered by other lipidomic tools and should powerfully assist in other nutritional, pathological or environmental contexts.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Silva JCP,Marques C,Martins FO,Viegas I,Tavares L,Macedo MP,Jones JGdoi
10.1016/j.ymben.2019.08.018subject
Has Abstractpub_date
2019-12-01 00:00:00pages
69-76eissn
1096-7176issn
1096-7184pii
S1096-7176(19)30196-Xjournal_volume
56pub_type
杂志文章abstract：:The chemical monomer p-hydroxystyrene (pHS) is used for producing a number of important industrial polymers from petroleum-based feedstocks. In an alternative approach, the microbial production of pHS can be envisioned by linking together a number of different metabolic pathways, of which those based on using glucose ...
journal_title：Metabolic engineering
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journal_title：Metabolic engineering
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pub_type： 杂志文章
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journal_title：Metabolic engineering
pub_type： 杂志文章,评审
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pub_type： 杂志文章
doi：10.1016/j.ymben.2013.12.005
更新日期：2014-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.ymben.2021.01.002
更新日期：2021-01-14 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2019.12.002
更新日期：2020-01-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2011.03.003
更新日期：2011-05-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2014.07.006
更新日期：2014-09-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2015.08.006
更新日期：2015-11-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2016.12.007
更新日期：2017-01-01 00:00:00
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journal_title：Metabolic engineering
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doi：10.1016/j.ymben.2006.06.004
更新日期：2006-11-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2018.11.009
更新日期：2019-03-01 00:00:00
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
pub_type： 杂志文章
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更新日期：2016-11-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
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更新日期：2018-07-01 00:00:00
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更新日期：2017-01-01 00:00:00
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doi：10.1016/j.ymben.2017.04.003
更新日期：2017-05-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章,评审
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更新日期：2016-11-01 00:00:00
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journal_title：Metabolic engineering
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doi：10.1016/j.ymben.2014.11.013
更新日期：2015-03-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章,评审
doi：10.1006/mben.2000.0168
更新日期：2001-01-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2020.04.001
更新日期：2020-07-01 00:00:00
