当前位置： SCI文献检索 > METABOLIC ENGINEERING期刊下所有文献 > Step changes in leaf oil accumulation via iterative metabolic engineering.

Step changes in leaf oil accumulation via iterative metabolic engineering.

Abstract：

:Synthesis and accumulation of plant oils in the entire vegetative biomass offers the potential to deliver yields surpassing those of oilseed crops. However, current levels still fall well short of those typically found in oilseeds. Here we show how transcriptome and biochemical analyses pointed to a futile cycle in a previously established Nicotiana tabacum line, accumulating up to 15% (dry weight) of the storage lipid triacylglycerol in leaf tissue. To overcome this metabolic bottleneck, we either silenced the SDP1 lipase or overexpressed the Arabidopsis thaliana LEC2 transcription factor in this transgenic background. Both strategies independently resulted in the accumulation of 30-33% triacylglycerol in leaf tissues. Our results demonstrate that the combined optimization of de novo fatty acid biosynthesis, storage lipid assembly and lipid turnover in leaf tissue results in a major overhaul of the plant central carbon allocation and lipid metabolism. The resulting further step changes in oil accumulation in the entire plant biomass offers the possibility of delivering yields that outperform current oilseed crops.

journal_name

Metab Eng

journal_title

Metabolic engineering

authors

Vanhercke T,Divi UK,El Tahchy A,Liu Q,Mitchell M,Taylor MC,Eastmond PJ,Bryant F,Mechanicos A,Blundell C,Zhi Y,Belide S,Shrestha P,Zhou XR,Ral JP,White RG,Green A,Singh SP,Petrie JR

doi

10.1016/j.ymben.2016.12.007

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

237-246

eissn

1096-7176

issn

1096-7184

pii

S1096-7176(16)30091-X

journal_volume

39

pub_type

杂志文章

相关文献

METABOLIC ENGINEERING文献大全
  • Metabolic engineering of Corynebacterium glutamicum for the de novo production of ethylene glycol from glucose.

    abstract：:Development of sustainable biological process for the production of bulk chemicals from renewable feedstock is an important goal of white biotechnology. Ethylene glycol (EG) is a large-volume commodity chemical with an annual production of over 20 million tons, and it is currently produced exclusively by petrochemical...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2015.10.013

    authors： Chen Z,Huang J,Wu Y,Liu D

    更新日期：2016-01-01 00:00:00

  • Combining Gal4p-mediated expression enhancement and directed evolution of isoprene synthase to improve isoprene production in Saccharomyces cerevisiae.

    abstract：:Current studies on microbial isoprene biosynthesis have mostly focused on regulation of the upstream mevalonic acid (MVA) or methyl-erythritol-4-phosphate (MEP) pathway. However, the downstream bottleneck restricting isoprene biosynthesis capacity caused by the weak expression and low activity of plant isoprene syntha...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2016.12.011

    authors： Wang F,Lv X,Xie W,Zhou P,Zhu Y,Yao Z,Yang C,Yang X,Ye L,Yu H

    更新日期：2017-01-01 00:00:00

  • Metabolic design for selective production of nicotinamide mononucleotide from glucose and nicotinamide.

    abstract：:β-Nicotinamide mononucleotide (NMN) is, one of the nucleotide compounds, a precursor of NAD+ and has recently attracted attention as a nutraceutical. Here, we develop a whole-cell biocatalyst using Escherichia coli, which enabled selective and effective high production of NMN from the inexpensive feedstock substrates ...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2020.11.008

    authors： Shoji S,Yamaji T,Makino H,Ishii J,Kondo A

    更新日期：2020-11-18 00:00:00

  • Production of muconic acid in plants.

    abstract：:Muconic acid (MA) is a dicarboxylic acid used for the production of industrially relevant chemicals such as adipic acid, terephthalic acid, and caprolactam. Because the synthesis of these polymer precursors generates toxic intermediates by utilizing petroleum-derived chemicals and corrosive catalysts, the development ...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2018.02.002

    authors： Eudes A,Berthomieu R,Hao Z,Zhao N,Benites VT,Baidoo EEK,Loqué D

    更新日期：2018-03-01 00:00:00

  • Metabolic flux analysis in Synechocystis using isotope distribution from 13C-labeled glucose.

    abstract：:Using the carbon isotope labeling technique, the response of cyanobacterial central carbon metabolism to the change in environmental conditions was investigated. Synechocystis was grown in the heterotrophic and mixotrophic cultures fed with 13C-labeled glucose. The labeling patterns of the amino acids in biomass hydro...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1006/mben.2002.0226

    authors： Yang C,Hua Q,Shimizu K

    更新日期：2002-07-01 00:00:00

  • Reconstitution of EPA and DHA biosynthesis in arabidopsis: iterative metabolic engineering for the synthesis of n-3 LC-PUFAs in transgenic plants.

    abstract：:An iterative approach to optimising the accumulation of non-native long chain polyunsaturated fatty acids in transgenic plants was undertaken in Arabidopsis thaliana. The contribution of a number of different transgene enzyme activities was systematically determined, as was the contribution of endogenous fatty acid me...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2013.03.001

    authors： Ruiz-Lopez N,Haslam RP,Usher SL,Napier JA,Sayanova O

    更新日期：2013-05-01 00:00:00

  • In silico assessment of the metabolic capabilities of an engineered functional reversal of the β-oxidation cycle for the synthesis of longer-chain (C≥4) products.

    abstract：:The modularity and versatility of an engineered functional reversal of the β-oxidation cycle make it a promising platform for the synthesis of longer-chain (C≥4) products. While the pathway has recently been exploited for the production of n-alcohols and carboxylic acids, fully capitalizing on its potential for the sy...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2014.02.011

    authors： Cintolesi A,Clomburg JM,Gonzalez R

    更新日期：2014-05-01 00:00:00

  • Complete genome sequence, metabolic model construction and phenotypic characterization of Geobacillus LC300, an extremely thermophilic, fast growing, xylose-utilizing bacterium.

    abstract：:We have isolated a new extremely thermophilic fast-growing Geobacillus strain that can efficiently utilize xylose, glucose, mannose and galactose for cell growth. When grown aerobically at 72 °C, Geobacillus LC300 has a growth rate of 2.15 h(-1) on glucose and 1.52 h(-1) on xylose (doubling time less than 30 min). The...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2015.09.009

    authors： Cordova LT,Long CP,Venkataramanan KP,Antoniewicz MR

    更新日期：2015-11-01 00:00:00

  • Metabolic engineering of isoprenoids.

    abstract：:The metabolic engineering of natural products has begun to prosper in the past few years due to genomic research and the discovery of biosynthetic genes. While the biosynthetic pathways and genes for some isoprenoids have been known for many years, new pathways have been found and known pathways have been further inve...

    journal_title：Metabolic engineering

    pub_type： 杂志文章,评审

    doi：10.1006/mben.2000.0168

    authors： Barkovich R,Liao JC

    更新日期：2001-01-01 00:00:00

  • Efficient production of saffron crocins and picrocrocin in Nicotiana benthamiana using a virus-driven system.

    abstract：:Crocins and picrocrocin are glycosylated apocarotenoids responsible, respectively, for the color and the unique taste of the saffron spice, known as red gold due to its high price. Several studies have also shown the health-promoting properties of these compounds. However, their high costs hamper the wide use of these...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2020.06.009

    authors： Martí M,Diretto G,Aragonés V,Frusciante S,Ahrazem O,Gómez-Gómez L,Daròs JA

    更新日期：2020-09-01 00:00:00

  • Glycosylation flux analysis reveals dynamic changes of intracellular glycosylation flux distribution in Chinese hamster ovary fed-batch cultures.

    abstract：:N-linked glycosylation of proteins has both functional and structural significance. Importantly, the glycan structure of a therapeutic protein influences its efficacy, pharmacokinetics, pharmacodynamics and immunogenicity. In this work, we developed glycosylation flux analysis (GFA) for predicting intracellular produc...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2017.07.005

    authors： Hutter S,Villiger TK,Brühlmann D,Stettler M,Broly H,Soos M,Gunawan R

    更新日期：2017-09-01 00:00:00

  • Tolerance and specificity of recombinant 6-methylsalicyclic acid synthase.

    abstract：BACKGROUND:6-Methylsalicylic acid synthase (MSAS), a fungal polyketide synthase from Penicillium patulum, is perhaps the simplest polyketide synthase that embodies several hallmarks of this family of multifunctional enzymes--a large multidomain protein, a high degree of specificity toward acetyl-CoA and malonyl-CoA sub...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1006/mben.1999.0113

    authors： Richardson MT,Pohl NL,Kealey JT,Khosla C

    更新日期：1999-04-01 00:00:00

  • Anaerobic production of medium-chain fatty alcohols via a β-reduction pathway.

    abstract：:In this report, we identify the relevant factors to increase production of medium chain n-alcohols through an expanded view of the reverse β-oxidation pathway. We began by creating a base strain capable of producing medium chain n-alcohols from glucose using a redox-balanced and growth-coupled metabolic engineering st...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2018.05.011

    authors： Mehrer CR,Incha MR,Politz MC,Pfleger BF

    更新日期：2018-07-01 00:00:00

  • Engineering Escherichia coli to produce branched-chain fatty acids in high percentages.

    abstract：:Branched-chain fatty acids (BCFAs) are key precursors of branched-chain fuels, which have cold-flow properties superior to straight chain fuels. BCFA production in Gram-negative bacterial hosts is inherently challenging because it competes directly with essential and efficient straight-chain fatty acid (SCFA) biosynth...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2016.07.003

    authors： Bentley GJ,Jiang W,Guamán LP,Xiao Y,Zhang F

    更新日期：2016-11-01 00:00:00

  • Ensemble Modeling for Robustness Analysis in engineering non-native metabolic pathways.

    abstract：:Metabolic pathways in cells must be sufficiently robust to tolerate fluctuations in expression levels and changes in environmental conditions. Perturbations in expression levels may lead to system failure due to the disappearance of a stable steady state. Increasing evidence has suggested that biological networks have...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2014.06.006

    authors： Lee Y,Lafontaine Rivera JG,Liao JC

    更新日期：2014-09-01 00:00:00

  • Metabolic engineering of Escherichia coli for production of L-aspartate and its derivative β-alanine with high stoichiometric yield.

    abstract：:L-aspartate is an important 4-carbon platform compound that can be used as the precursor of numerous chemical products. The bioproduction of L-aspartate directly from biomass resources is expected to provide a more cost-competitive technique route. Yet little metabolic engineering work on this matter has been carried ...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2019.04.012

    authors： Piao X,Wang L,Lin B,Chen H,Liu W,Tao Y

    更新日期：2019-07-01 00:00:00

  • Iterative algorithm-guided design of massive strain libraries, applied to itaconic acid production in yeast.

    abstract：:Metabolic engineering requires multiple rounds of strain construction to evaluate alternative pathways and enzyme concentrations. Optimizing multigene pathways stepwise or by randomly selecting enzymes and expression levels is inefficient. Here, we apply methods from design of experiments (DOE) to guide the constructi...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2018.05.002

    authors： Young EM,Zhao Z,Gielesen BEM,Wu L,Benjamin Gordon D,Roubos JA,Voigt CA

    更新日期：2018-07-01 00:00:00

  • High-yield whole cell biosynthesis of Nylon 12 monomer with self-sufficient supply of multiple cofactors.

    abstract：:Biosynthesis of Nylon 12 monomer using dodecanoic acid (DDA) or its esters as the renewable feedstock typically involves ω-hydroxylation, oxidation and ω-amination. The dependence of hydroxylation and oxidation-catalyzing enzymes on redox cofactors, and the requirement of L-alanine as the co-substrate and pyridoxal 5'...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2020.09.006

    authors： Ge J,Yang X,Yu H,Ye L

    更新日期：2020-11-01 00:00:00

  • Mapping photoautotrophic metabolism with isotopically nonstationary (13)C flux analysis.

    abstract：:Understanding in vivo regulation of photoautotrophic metabolism is important for identifying strategies to improve photosynthetic efficiency or re-route carbon fluxes to desirable end products. We have developed an approach to reconstruct comprehensive flux maps of photoautotrophic metabolism by computational analysis...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2011.08.002

    authors： Young JD,Shastri AA,Stephanopoulos G,Morgan JA

    更新日期：2011-11-01 00:00:00

  • A phycocyanin·phellandrene synthase fusion enhances recombinant protein expression and β-phellandrene (monoterpene) hydrocarbons production in Synechocystis (cyanobacteria).

    abstract：:Cyanobacteria can be exploited as photosynthetic platforms for heterologous generation of terpene hydrocarbons with industrial applications. Transformation of Synechocystis and heterologous expression of the β-phellandrene synthase (PHLS) gene alone is necessary and sufficient to confer to Synechocystis the ability to...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2015.09.010

    authors： Formighieri C,Melis A

    更新日期：2015-11-01 00:00:00

  • A metabolic network analysis & NMR experiment design tool with user interface-driven model construction for depth-first search analysis.

    abstract：:A Windows program for metabolic engineering analysis and experimental design has been developed. A graphical user interface enables the pictorial, "on-screen" construction of a metabolic network. Once a model is composed, balance equations are automatically generated. Model construction, modification and information e...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/s1096-7176(03)00023-5

    authors： Zhu T,Phalakornkule C,Ghosh S,Grossmann IE,Koepsel RR,Ataai MM,Domach MM

    更新日期：2003-04-01 00:00:00

  • Review of methods to probe single cell metabolism and bioenergetics.

    abstract：:Single cell investigations have enabled unexpected discoveries, such as the existence of biological noise and phenotypic switching in infection, metabolism and treatment. Herein, we review methods that enable such single cell investigations specific to metabolism and bioenergetics. Firstly, we discuss how to isolate a...

    journal_title：Metabolic engineering

    pub_type： 杂志文章,评审

    doi：10.1016/j.ymben.2014.09.007

    authors： Vasdekis AE,Stephanopoulos G

    更新日期：2015-01-01 00:00:00

  • Construction of lycopene-overproducing Saccharomyces cerevisiae by combining directed evolution and metabolic engineering.

    abstract：:Improved supply of farnesyl diphosphate (FPP) is often considered as a typical strategy for engineering Saccharomyces cerevisiae towards efficient terpenoid production. However, in the engineered strains with enhanced precursor supply, the production of the target metabolite is often impeded by insufficient capacity o...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2015.04.009

    authors： Xie W,Lv X,Ye L,Zhou P,Yu H

    更新日期：2015-07-01 00:00:00

  • Dynamic consolidated bioprocessing for direct production of xylonate and shikimate from xylan by Escherichia coli.

    abstract：:Numerous value-added chemicals can be produced using xylan as a feedstock. However, the product yields are limited by low xylan utilization efficiency, as well as by carbon flux competition between biomass production and biosynthesis. Herein, a dynamic consolidated bioprocessing strategy was developed, which coupled x...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2020.04.001

    authors： Gao C,Guo L,Ding Q,Hu G,Ye C,Liu J,Chen X,Liu L

    更新日期：2020-07-01 00:00:00

  • The organization of metabolic reaction networks. II. Signal processing in hierarchical structured functional units.

    abstract：:Based on the analysis of molecular interactions of proteins with DNA binding sites, a new approach to developing mathematical models describing gene expression is introduced. Detection of hierarchical structures in metabolic networks can be used to decompose complex reaction schemes. This will be achieved by assigning...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1006/mben.2000.0175

    authors： Kremling A,Gilles ED

    更新日期：2001-04-01 00:00:00

  • Glutamate excretion as a major kinetic bottleneck for the thermally triggered production of glutamic acid by Corynebacterium glutamicum.

    abstract：:The study was aimed at evaluating the extent of flux control exercised by the amino acid excretion step on the glutamate production flux in C. glutamicum 2262 strain that is induced for glutamate excretion by an upward temperature shift. Cells initially induced to excrete glutamate were cultivated at different control...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1006/mben.1999.0129

    authors： Lapujade P,Goergen JL,Engasser JM

    更新日期：1999-07-01 00:00:00

  • Determining contributions of exogenous glucose and fructose to de novo fatty acid and glycerol synthesis in liver and adipose tissue.

    abstract：:The de novo synthesis of triglyceride (TG) fatty acids (FA) and glycerol can be measured with stable isotope tracers. However, these methods typically do not inform the contribution of a given substrate to specific pathways on these synthetic processes. We integrated deuterated water (2H2O) measurement of de novo lipo...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2019.08.018

    authors： Silva JCP,Marques C,Martins FO,Viegas I,Tavares L,Macedo MP,Jones JG

    更新日期：2019-12-01 00:00:00

  • Generation of serum-stabilized retroviruses: reduction of alpha1,3gal-epitope synthesis in a murine NIH3T3-derived packaging cell line by expression of chimeric glycosyltransferases.

    abstract：:Retroviral vectors released from mouse-derived packaging cell lines are inactivated in human sera by naturally occurring antibodies due to the recognition of Galalpha1,3Galbeta1,4GlcNAc (alphagal-epitope) decorated surface proteins. In this study, an extensive analysis of the glycosylation potential of NIH3T3-derived ...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2005.02.004

    authors： Hansen W,Grabenhorst E,Nimtz M,Müller K,Conradt HS,Wirth M

    更新日期：2005-05-01 00:00:00

  • Minimization of glycerol synthesis in industrial ethanol yeast without influencing its fermentation performance.

    abstract：:To synthesize glycerol, a major by-product during anaerobic production of ethanol, the yeast Saccharomyces cerevisiae would consume up to 4% of the sugar feedstock in typical industrial ethanol processes. The present study was dedicated to decreasing the glycerol production mostly in industrial ethanol producing yeast...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2010.11.003

    authors： Guo ZP,Zhang L,Ding ZY,Shi GY

    更新日期：2011-01-01 00:00:00

  • Engineered citrate synthase alters Acetate Accumulation in Escherichia coli.

    abstract：:Metabolic engineering is used to improve titers, yields and generation rates for biochemical products in host microbes such as Escherichia coli. A wide range of biochemicals are derived from the central carbon metabolite acetyl-CoA, and the largest native drain of acetyl-CoA in most microbes including E. coli is entry...

    journal_title：Metabolic engineering

    pub_type： 杂志文章

    doi：10.1016/j.ymben.2020.06.006

    authors： Tovilla-Coutiño DB,Momany C,Eiteman MA

    更新日期：2020-09-01 00:00:00