Abstract：
:Development of sustainable biological process for the production of bulk chemicals from renewable feedstock is an important goal of white biotechnology. Ethylene glycol (EG) is a large-volume commodity chemical with an annual production of over 20 million tons, and it is currently produced exclusively by petrochemical route. Herein, we report a novel biosynthetic route to produce EG from glucose by the extension of serine synthesis pathway of Corynebacterium glutamicum. The EG synthesis is achieved by the reduction of glycoaldehyde derived from serine. The transformation of serine to glycoaldehyde is catalyzed either by the sequential enzymatic deamination and decarboxylation or by the enzymatic decarboxylation and oxidation. We screened the corresponding enzymes and optimized the production strain by combinatorial optimization and metabolic engineering. The best engineered C. glutamicum strain is able to accumulate 3.5 g/L of EG with the yield of 0.25 mol/mol glucose in batch cultivation. This study lays the basis for developing an efficient biological process for EG production.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Chen Z,Huang J,Wu Y,Liu Ddoi
10.1016/j.ymben.2015.10.013subject
Has Abstractpub_date
2016-01-01 00:00:00pages
12-18eissn
1096-7176issn
1096-7184pii
S1096-7176(15)00149-4journal_volume
33pub_type
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
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pub_type： 杂志文章
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更新日期：2006-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1006/mben.2002.0226
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1006/mben.1999.0129
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2015.10.003
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doi：10.1016/j.ymben.2006.05.007
更新日期：2006-11-01 00:00:00
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更新日期：2015-03-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2018.03.007
更新日期：2018-05-01 00:00:00
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pub_type： 杂志文章,评审
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journal_title：Metabolic engineering
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更新日期：2006-07-01 00:00:00
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doi：10.1016/j.ymben.2017.07.003
更新日期：2017-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1006/mben.2001.0199
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更新日期：2017-07-01 00:00:00
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更新日期：2005-03-01 00:00:00
