Abstract：
:There is considerable interest in mining organisms for new natural products (NPs) and in improving methods to overproduce valuable NPs. Because of the rapid development of tools and strategies for metabolic engineering and the markedly increased knowledge of the biosynthetic pathways and genetics of NP-producing organisms, genome mining and overproduction of NPs can be dramatically accelerated. In particular, Streptomyces species have been proposed as suitable chassis organisms for NP discovery and overproduction because of their many unique characteristics not shared with yeast, Escherichia coli, or other microorganisms. In this review, we summarize the methods for genome sequencing, gene cluster prediction, and gene editing in Streptomyces, as well as metabolic engineering strategies for NP overproduction and approaches for generating new products. Finally, two strategies for utilizing Streptomyces as the chassis for NP discovery and overproduction are emphasized.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Liu R,Deng Z,Liu Tdoi
10.1016/j.ymben.2018.05.015subject
Has Abstractpub_date
2018-11-01 00:00:00pages
74-84eissn
1096-7176issn
1096-7184pii
S1096-7176(18)30035-1journal_volume
50pub_type
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