Abstract:
:We generated a genomic library from sheared Clostridium acetobutylicum ATCC 824 DNA, whereby inserts can be expressed in both directions from the thiolase promoter, P(thl). Serial transfer of library-bearing C. acetobutylicum cultures exposed to increasing butyrate concentrations enriched for inserts containing fragments of rRNA genetic loci. The selected library inserts were placed so that antisense (to the rRNAs) non-coding RNAs (ncRNAs) would be transcribed from P(thl). Different enriched inserts imparted similar butyrate-tolerance characteristics. A minimal tolerance fragment (RDNA7) was identified as the 16S-rRNA promoter region. Expressed on plasmid pRD7 off P(thl), RDNA7 can produce putative ncRNAs termed ncRNA(RD7). C. acetobutylicum 824(pRD7) showed superior resistance to butyrate and other carboxylic acids. Transcriptional analysis of butyrate stress identified 120 differentially expressed genes between 824(pRD7) and 824(pSOS95del). The few upregulated genes included the ffh gene of the putative signal recognition particle (SRP) system. Northern analysis of ncRNA(RD7) and corresponding antisense RNAs demonstrated multiple ncRNA(RD7) molecules in 824(pRD7). Several corresponding antisense RNA molecules were identified both in 824(pRD7) and 824(pSOS95del), but at much higher levels in 824(pRD7). Northern analysis of 16S rRNA expression suggested complex RDNA7-dependent rRNA processing. Our data suggest that by hybridizing against unprocessed rRNA precursors, ncRNA(RD7) alters rRNA processing, and these alterations result in acid tolerance, possibly through a mechanism involving the Ffh protein.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Borden JR,Jones SW,Indurthi D,Chen Y,Papoutsakis ETdoi
10.1016/j.ymben.2009.12.004subject
Has Abstractpub_date
2010-05-01 00:00:00pages
268-81issue
3eissn
1096-7176issn
1096-7184pii
S1096-7176(09)00110-4journal_volume
12pub_type
杂志文章abstract::A mathematical model to describe carbon catabolite repression in Escherichia coli is developed and in part validated. The model is aggregated from two functional units describing glucose and lactose transport and degradation. Both units are members of the crp modulon and are under control of a global signal transducti...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1006/mben.2001.0199
更新日期:2001-10-01 00:00:00
abstract::To mimic the delicately regulated metabolism in nature for improved efficiency, artificial and customized regulatory components for dynamically controlling metabolic networks in multiple layers are essential in laboratory engineering. For this purpose, a novel regulatory component for controlling vanillin biosynthetic...
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journal_title:Metabolic engineering
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doi:10.1016/j.ymben.2018.09.005
更新日期:2019-01-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2013.07.001
更新日期:2013-09-01 00:00:00
abstract::In this study strains of Ralstonia eutropha H16 and Pseudomonas putida KT2440 were engineered which are suitable for biotechnological production of 2-methylcitric acid (2MC). Analysis of a previous mutant of R. eutropha able to accumulate 2MC recommended this strain as a candidate for fermentative production of 2MC. T...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2006.05.007
更新日期:2006-11-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2018.05.021
更新日期:2018-07-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2014.02.011
更新日期:2014-05-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2014.07.006
更新日期:2014-09-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2010.12.003
更新日期:2011-03-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2016.12.007
更新日期:2017-01-01 00:00:00
abstract::FK506, a widely used immunosuppressant, is produced by industrial fermentation processes using various Streptomyces species. Independently of the strain, structurally related compound FK520 is co-produced, resulting in complex and costly isolation procedures. In this paper, we report a chemobiosynthetic approach for e...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2011.11.003
更新日期:2012-01-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2015.10.013
更新日期:2016-01-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2018.11.006
更新日期:2019-03-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2020.04.001
更新日期:2020-07-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2017.11.005
更新日期:2018-01-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2009.11.001
更新日期:2010-05-01 00:00:00
abstract::Global gene regulation throughout the Escherichia coli stress response to overexpression of each of five recombinant proteins was evaluated. Reverse-transcriptase polymerase chain reaction-amplified mRNA from induced and control cells were hybridized with a DNA array of Kohara clones representing 16% (700 genes) of th...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1006/mben.2000.0148
更新日期:2000-07-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2017.07.004
更新日期:2017-09-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2015.08.003
更新日期:2015-09-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章,评审
doi:10.1006/mben.2000.0168
更新日期:2001-01-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2018.05.008
更新日期:2018-07-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1006/mben.1999.0141
更新日期:2000-04-01 00:00:00
abstract::Klebsiella oxytoca KMS005 (∆adhE∆ackA-pta∆ldhA) was metabolically engineered to improve 2,3-butanediol (BDO) yield. Elimination of alcohol dehydrogenase E (adhE), acetate kinase A-phosphotransacetylase (ackA-pta), and lactate dehydrogenase A (ldhA) enzymes allowed BDO production as a primary pathway for NADH re-oxidat...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2015.04.004
更新日期:2015-07-01 00:00:00
abstract::Fatty acids are the most abundant form of reduced carbon chains available from nature and have diverse uses ranging from food to industrial feedstocks. Plants represent a significant renewable source of fatty acids because many species accumulate them in the form of triacylglycerol as major storage components in seeds...
journal_title:Metabolic engineering
pub_type: 杂志文章,评审
doi:10.1006/mben.2001.0204
更新日期:2002-01-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2005.10.001
更新日期:2006-03-01 00:00:00
abstract::This study focused on metabolic changes in the neuronal human cell line AGE1.HN upon increased ammonia stress. Batch cultivations of α(1)-antitrypsin (A1AT) producing AGE1.HN cells were carried out in media with initial ammonia concentrations ranging from 0mM to 5mM. Growth, A1AT production, metabolite dynamics and fi...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2012.01.001
更新日期:2012-03-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2019.10.006
更新日期:2020-01-01 00:00:00
abstract::We report improved NADPH flux and xylitol biosynthesis in engineered E. coli. Xylitol is produced from xylose via an NADPH dependent reductase. We utilize 2-stage dynamic metabolic control to compare two approaches to optimize xylitol biosynthesis, a stoichiometric approach, wherein competitive fluxes are decreased, a...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2021.01.005
更新日期:2021-01-16 00:00:00
abstract::Whereas microbial fermentation processes for producing ethanol and related alcohol biofuels are well established, biodiesel (methyl esters of fatty acids) is exclusively derived from plant oils. Slow cycle times for engineering oilseed metabolism and the excessive accumulation of glycerol as a byproduct are two major ...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2008.08.006
更新日期:2008-11-01 00:00:00