Abstract:
:Ethyl acetate is an industrially relevant ester that is currently produced exclusively through unsustainable processes. Many yeasts are able to produce ethyl acetate, but the main responsible enzyme has remained elusive, hampering the engineering of novel production strains. Here we describe the discovery of a new enzyme (Eat1) from the yeast Wickerhamomyces anomalus that resulted in high ethyl acetate production when expressed in Saccharomyces cerevisiae and Escherichia coli. Purified Eat1 showed alcohol acetyltransferase activity with ethanol and acetyl-CoA. Homologs of eat1 are responsible for most ethyl acetate synthesis in known ethyl acetate-producing yeasts, including S. cerevisiae, and are only distantly related to known alcohol acetyltransferases. Eat1 is therefore proposed to compose a novel alcohol acetyltransferase family within the α/β hydrolase superfamily. The discovery of this novel enzyme family is a crucial step towards the development of biobased ethyl acetate production and will also help in selecting improved S. cerevisiae brewing strains.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Kruis AJ,Levisson M,Mars AE,van der Ploeg M,Garcés Daza F,Ellena V,Kengen SWM,van der Oost J,Weusthuis RAdoi
10.1016/j.ymben.2017.03.004subject
Has Abstractpub_date
2017-05-01 00:00:00pages
92-101eissn
1096-7176issn
1096-7184pii
S1096-7176(17)30032-0journal_volume
41pub_type
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