Abstract：
:The non-recyclable use of nitrogen fertilizers in microbial production of fuels and chemicals remains environmentally detrimental. Conversion of protein wastes into biofuels and ammonia by engineering nitrogen flux in Escherichia coli has been demonstrated as a method to reclaim reduced-nitrogen and curb its environmental deposition. However, protein biomass requires a proteolysis process before it can be taken up and converted by any microbe. Here, we metabolically engineered Bacillus subtilis to hydrolyze polypeptides through its secreted proteases and to convert amino acids into advanced biofuels and ammonia fertilizer. Redirection of B. subtilis metabolism for amino-acid conversion required inactivation of the branched-chain amino-acid (BCAA) global regulator CodY. Additionally, the lipoamide acyltransferase (bkdB) was deleted to prevent conversion of branched-chain 2-keto acids into their acyl-CoA derivatives. With these deletions and heterologous expression of a keto-acid decarboxylase and an alcohol dehydrogenase, the final strain produced biofuels and ammonia from an amino-acid media with 18.9% and 46.6% of the maximum theoretical yield. The process was also demonstrated on several waste proteins. The results demonstrate the feasibility of direct microbial conversion of polypeptides into sustainable products.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Choi KY,Wernick DG,Tat CA,Liao JCdoi
10.1016/j.ymben.2014.02.007subject
Has Abstractpub_date
2014-05-01 00:00:00pages
53-61eissn
1096-7176issn
1096-7184pii
S1096-7176(14)00021-4journal_volume
23pub_type
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
pub_type： 杂志文章,评审
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
pub_type： 杂志文章
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2019.03.006
更新日期：2019-07-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2017.07.005
更新日期：2017-09-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2011.08.002
更新日期：2011-11-01 00:00:00
abstract：:Biotin is an archetypal vitamin used as cofactor for carboxylation reactions found in all forms of life. However, biotin biosynthesis is an elaborate multi-enzymatic process and metabolically costly. Moreover, many industrially relevant organisms are incapable of biotin synthesis resulting in the requirement to supple...
journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2016.12.013
更新日期：2017-03-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2019.10.006
更新日期：2020-01-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章,评审
doi：10.1016/j.ymben.2014.11.005
更新日期：2015-01-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2017.10.012
更新日期：2017-11-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章,评审
doi：10.1016/j.ymben.2003.10.008
更新日期：2004-01-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2018.05.002
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2012.08.006
更新日期：2012-11-01 00:00:00
abstract：:Although CRISPR-Cas9/Cpf1 have been employed as powerful genome engineering tools, heterologous CRISPR-Cas9/Cpf1 are often difficult to introduce into bacteria and archaea due to their severe toxicity. Since most prokaryotes harbor native CRISPR-Cas systems, genome engineering can be achieved by harnessing these endog...
journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2018.03.007
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journal_title：Metabolic engineering
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更新日期：2021-01-16 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2015.08.006
更新日期：2015-11-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2019.07.012
更新日期：2020-01-01 00:00:00
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journal_title：Metabolic engineering
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更新日期：2020-07-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2019.07.002
更新日期：2019-09-01 00:00:00
abstract：:Based on the analysis of molecular interactions of proteins with DNA binding sites, a new approach to developing mathematical models describing gene expression is introduced. Detection of hierarchical structures in metabolic networks can be used to decompose complex reaction schemes. This will be achieved by assigning...
journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1006/mben.2000.0175
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