Abstract：
:Stress hormones and pro-inflammatory cytokines are putative signals triggering increased energy expenditure or "hypermetabolism" commonly observed in inflammatory states. Cytokines also cause the release of reactive oxidants by immune cells resident in tissues in vivo. Therefore, we hypothesized that oxidative stress plays a role in the induction of hypermetabolism. We examined the effect of glucagon (1.0 nM), a catabolic stress hormone, and the oxidant H(2)O(2) (1.0 mM) on the metabolism of stable hepatocyte cultures for 4 days. Combined H(2)O(2) and glucagon treatment, but not H(2)O(2) or glucagon used alone, increased the hepatocyte oxygen uptake rate 25% above control untreated cells after a lag-time of 72 h. The same treatment also increased the expression of mitochondrial uncoupling protein-2 (UCP2). These effects were significantly inhibited by the antioxidant N-acetylcysteine (5mM) and the pentose phosphate pathway (PPP) inhibitor dehydroepianderosterone (200 microM). Glucagon alone induced urea synthesis and H(2)O(2) alone induced the PPP. These findings show, for the first time, that oxidative stress, in combination with glucagon, increases metabolic energy expenditure in cultured cells, and that this effect may be mediated by UCP-2. Furthermore, the results implicate the PPP in the induction of the hypermetabolic response.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Lee K,Berthiaume F,Stephanopoulos GN,Yarmush MLdoi
10.1016/s1096-7176(03)00042-9subject
Has Abstractpub_date
2003-10-01 00:00:00pages
221-9issue
4eissn
1096-7176issn
1096-7184pii
S1096717603000429journal_volume
5pub_type
杂志文章abstract：:Geobacter species are among the most effective microorganisms known for the bioremediation of radioactive and toxic metals in contaminated subsurface environments and for converting organic compounds to electricity in microbial fuel cells. However, faster rates of electron transfer could aid in optimizing these proces...
journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2008.06.005
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journal_title：Metabolic engineering
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doi：10.1016/j.ymben.2012.06.001
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1006/mben.2000.0175
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journal_title：Metabolic engineering
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doi：10.1016/j.ymben.2015.09.009
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doi：10.1016/j.ymben.2010.03.002
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pub_type： 杂志文章,评审
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2017.06.002
更新日期：2017-07-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章,评审
doi：10.1006/mben.2001.0204
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journal_title：Metabolic engineering
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doi：10.1016/j.ymben.2011.03.003
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2006.05.007
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journal_title：Metabolic engineering
pub_type： 杂志文章,评审
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更新日期：2012-07-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2004.10.003
更新日期：2005-03-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2012.01.001
更新日期：2012-03-01 00:00:00
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更新日期：2020-11-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2015.03.012
更新日期：2015-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2011-11-01 00:00:00
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pub_type： 杂志文章
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journal_title：Metabolic engineering
pub_type： 杂志文章,评审
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doi：10.1016/j.ymben.2018.02.002
更新日期：2018-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.ymben.2017.10.003
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journal_title：Metabolic engineering
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