Abstract:
:The introduction of a synthetic metabolic pathway consisting of multiple genes derived from various organisms enables cyanobacteria to directly produce valuable chemicals from carbon dioxide. We previously constructed a synthetic metabolic pathway composed of genes from Escherichia coli, Saccharomyces cerevisiae, and Klebsiella pneumoniae. This pathway enabled 1,3-propanediol (1,3-PDO) production from cellular DHAP via glycerol in the cyanobacterium, Synechococcus elongatus PCC 7942. The production of 1,3-PDO (3.79mM, 0.29g/l) directly from carbon dioxide by engineered S. elongatus PCC 7942 was successfully accomplished. However, the constructed strain accumulated a remarkable amount of glycerol (12.6mM, 1.16g/l), an intermediate metabolite in 1,3-PDO production. Notably, enhancement of latter reactions of synthetic metabolic pathway for conversion of glycerol to 1,3-PDO increases 1,3-PDO production. In this study, we aimed to increase the observed 1,3-PDO production titer. First, the weaker S. elongatus PCC 7942 promoter, PLlacO1, was replaced with a stronger promoter (Ptrc) to regulate genes involved in the conversion of glycerol to 1,3-PDO. Second, the induction timing for gene expression and medium composition were optimized. Promoter replacement resulted in higher 1,3-PDO production than glycerol accumulation, and the amount of products (1,3-PDO and glycerol) generated via the synthetic metabolic pathway increased with optimization of medium composition. Accordingly, we achieved the highest titer of 1,3-PDO (16.1mM, 1.22g/l) and this was higher than glycerol accumulation (9.46mM, 0.87g/l). The improved titer was over 4-fold higher than that of our previous study.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Hirokawa Y,Maki Y,Hanai Tdoi
10.1016/j.ymben.2016.12.001subject
Has Abstractpub_date
2017-01-01 00:00:00pages
192-199eissn
1096-7176issn
1096-7184pii
S1096-7176(16)30146-Xjournal_volume
39pub_type
杂志文章abstract::To synthesize glycerol, a major by-product during anaerobic production of ethanol, the yeast Saccharomyces cerevisiae would consume up to 4% of the sugar feedstock in typical industrial ethanol processes. The present study was dedicated to decreasing the glycerol production mostly in industrial ethanol producing yeast...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2010.11.003
更新日期:2011-01-01 00:00:00
abstract::Retroviral vectors released from mouse-derived packaging cell lines are inactivated in human sera by naturally occurring antibodies due to the recognition of Galalpha1,3Galbeta1,4GlcNAc (alphagal-epitope) decorated surface proteins. In this study, an extensive analysis of the glycosylation potential of NIH3T3-derived ...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2005.02.004
更新日期:2005-05-01 00:00:00
abstract::To obtain fast growing oil-rich microalgal strains has been urgently demanded for microalgal biofuel. Malic enzyme (ME), which is involved in pyruvate metabolism and carbon fixation, was first characterized in microalgae here. Overexpression of Phaeodactylum tricornutum ME (PtME) significantly enhanced the expression ...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2014.10.002
更新日期:2015-01-01 00:00:00
abstract::A Chinese hamster ovary (CHO) cell line producing recombinant human bone morphogenetic protein-4 (rhBMP-4) (CHO-BMP-4), which expresses essential components of BMP signal transduction, underwent autocrine BMP-4 signaling. RNA seq analysis on CHO host cells (DG44) treated with rhBMP-4 (20 µg/mL) suggested that rhBMP-4 ...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2018.11.003
更新日期:2019-03-01 00:00:00
abstract::Succinic acid is a platform chemical of recognized industrial value and accordingly faces a continuous challenge to enable manufacturing from most attractive raw materials. It is mainly produced from glucose, using microbial fermentation. Here, we explore and optimize succinate production from sucrose, a globally appl...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2017.10.003
更新日期:2017-11-01 00:00:00
abstract::Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is a promising biopolyester with good mechanical properties and biodegradability. Large-scale production of PHBV is still hindered by the high production cost. CRISPR/Cas9 method was used to engineer the TCA cycle in Halomonas bluephagenesis on its chromosome for pro...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2019.03.006
更新日期:2019-07-01 00:00:00
abstract::Understanding the metabolic and regulatory pathways of hepatocytes is important for biotechnological applications involving liver cells. Previous attempts to culture hepatocytes in plasma yielded poor functional results. Recently we reported that hormone (insulin and hydrocortisone) and amino acid supplementation redu...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/s1096-7176(02)00011-3
更新日期:2003-01-01 00:00:00
abstract::We generated a genomic library from sheared Clostridium acetobutylicum ATCC 824 DNA, whereby inserts can be expressed in both directions from the thiolase promoter, P(thl). Serial transfer of library-bearing C. acetobutylicum cultures exposed to increasing butyrate concentrations enriched for inserts containing fragme...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2009.12.004
更新日期:2010-05-01 00:00:00
abstract::Rational metabolic engineering methods are increasingly employed in designing the commercially viable processes for the production of chemicals relevant to pharmaceutical, biotechnology, and food and beverage industries. With the growing availability of omics data and of methodologies capable to integrate the availabl...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2016.01.009
更新日期:2016-05-01 00:00:00
abstract::β-Nicotinamide mononucleotide (NMN) is, one of the nucleotide compounds, a precursor of NAD+ and has recently attracted attention as a nutraceutical. Here, we develop a whole-cell biocatalyst using Escherichia coli, which enabled selective and effective high production of NMN from the inexpensive feedstock substrates ...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2020.11.008
更新日期:2020-11-18 00:00:00
abstract::Saccharomyces cerevisiae is an efficient host for natural-compound production and preferentially employed in academic studies and bioindustries. However, S. cerevisiae exhibits limited production capacity for lipophilic natural products, especially compounds that accumulate intracellularly, such as polyketides and car...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2018.11.009
更新日期:2019-03-01 00:00:00
abstract::The potential to produce succinate aerobically in Escherichia coli would offer great advantages over anaerobic fermentation in terms of faster biomass generation, carbon throughput, and product formation. Genetic manipulations were performed on two aerobic succinate production systems to increase their succinate yield...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2004.10.003
更新日期:2005-03-01 00:00:00
abstract::Acid-tolerant Saccharomyces cerevisiae was engineered to produce lactic acid by expressing heterologous lactate dehydrogenase (LDH) genes, while attenuating several key pathway genes, including glycerol-3-phosphate dehydrogenase1 (GPD1) and cytochrome-c oxidoreductase2 (CYB2). In order to increase the yield of lactic ...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2015.09.006
更新日期:2016-05-01 00:00:00
abstract::5-Aminovalerate (5AVA) is the precursor of valerolactam, a potential building block for producing nylon 5, and is a C5 platform chemical for synthesizing 5-hydroxyvalerate, glutarate, and 1,5-pentanediol. Escherichia coli was metabolically engineered for the production of 5-aminovalerate (5AVA) and glutarate. When the...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2012.11.011
更新日期:2013-03-01 00:00:00
abstract::Control of gene and protein expression of both endogenous and heterologous genes is a key component of metabolic engineering. While a large amount of work has been published characterizing promoters for this purpose, less effort has been exerted to elucidate the role of terminators in yeast. In this study, we characte...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2013.07.001
更新日期:2013-09-01 00:00:00
abstract::Metabolic engineering requires multiple rounds of strain construction to evaluate alternative pathways and enzyme concentrations. Optimizing multigene pathways stepwise or by randomly selecting enzymes and expression levels is inefficient. Here, we apply methods from design of experiments (DOE) to guide the constructi...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2018.05.002
更新日期:2018-07-01 00:00:00
abstract::Klebsiella oxytoca KMS005 (∆adhE∆ackA-pta∆ldhA) was metabolically engineered to improve 2,3-butanediol (BDO) yield. Elimination of alcohol dehydrogenase E (adhE), acetate kinase A-phosphotransacetylase (ackA-pta), and lactate dehydrogenase A (ldhA) enzymes allowed BDO production as a primary pathway for NADH re-oxidat...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2015.04.004
更新日期:2015-07-01 00:00:00
abstract::Cyclic guanosine monophosphate (cGMP) is a universal second messenger that is synthesized from guanosine triphosphate (GTP) by guanylyl cyclases (GCs) and hydrolyzed into guanosine monophosphate (GMP) by phosphodiesterases (PDEs). Small-molecule drugs that induce high cGMP levels in specialized tissues by boosting GC ...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2015.03.016
更新日期:2015-05-01 00:00:00
abstract::Pyocyanin is a secondary metabolite from Pseudomonas aeruginosa that belongs to the class of phenazines, which are aromatic nitrogenous compounds with numerous biological functions. Besides its antifungal and antimicrobial activities, pyocyanin is a remarkable redox-active molecule with potential applications ranging ...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2021.01.002
更新日期:2021-01-14 00:00:00
abstract::Although CRISPR-Cas9/Cpf1 have been employed as powerful genome engineering tools, heterologous CRISPR-Cas9/Cpf1 are often difficult to introduce into bacteria and archaea due to their severe toxicity. Since most prokaryotes harbor native CRISPR-Cas systems, genome engineering can be achieved by harnessing these endog...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2018.03.007
更新日期:2018-05-01 00:00:00
abstract::Successful redirection of endogenous resources into heterologous pathways is a central tenet in the creation of efficient microbial cell factories. This redirection, however, may come at a price of poor biomass accumulation, reduced cofactor regeneration and low recombinant enzyme expression. In this study, we propose...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2012.08.006
更新日期:2012-11-01 00:00:00
abstract::Styrene is an important petroleum-derived molecule that is polymerized to make versatile plastics, including disposable silverware and foamed packaging materials. Finding more sustainable methods, such as biosynthesis, for producing styrene is essential due to the increasing severity of climate change as well as the l...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2020.05.009
更新日期:2020-09-01 00:00:00
abstract::Improved supply of farnesyl diphosphate (FPP) is often considered as a typical strategy for engineering Saccharomyces cerevisiae towards efficient terpenoid production. However, in the engineered strains with enhanced precursor supply, the production of the target metabolite is often impeded by insufficient capacity o...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2015.04.009
更新日期:2015-07-01 00:00:00
abstract::FK506, a widely used immunosuppressant, is produced by industrial fermentation processes using various Streptomyces species. Independently of the strain, structurally related compound FK520 is co-produced, resulting in complex and costly isolation procedures. In this paper, we report a chemobiosynthetic approach for e...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2011.11.003
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:6-Methylsalicylic acid synthase (MSAS), a fungal polyketide synthase from Penicillium patulum, is perhaps the simplest polyketide synthase that embodies several hallmarks of this family of multifunctional enzymes--a large multidomain protein, a high degree of specificity toward acetyl-CoA and malonyl-CoA sub...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1006/mben.1999.0113
更新日期:1999-04-01 00:00:00
abstract::Dietary carotenoids have been shown to be beneficial to health by decreasing the risk of many diseases. Attempts to enhance carotenoids in food crops have been successful although higher plants appear to resist big changes of carotenoid biosynthesis by metabolic engineering. Here we report the generation of a more nut...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2013.02.005
更新日期:2013-05-01 00:00:00
abstract::In this report, UbiE and UbiH in the quinone modification pathway (QMP) were identified in addition to UbiG as bottleneck enzymes in the CoQ10 biosynthesis by Rhodobacter sphaeroides. The CoQ10 content was enhanced after co-overexpression of UbiE and UbiG, however, accompanied by the accumulation of the intermediate 1...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2015.03.012
更新日期:2015-05-01 00:00:00
abstract::Using the carbon isotope labeling technique, the response of cyanobacterial central carbon metabolism to the change in environmental conditions was investigated. Synechocystis was grown in the heterotrophic and mixotrophic cultures fed with 13C-labeled glucose. The labeling patterns of the amino acids in biomass hydro...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1006/mben.2002.0226
更新日期:2002-07-01 00:00:00
abstract::The complete genome sequence of the filamentous fungi Aspergillus nidulans has paved the way for fundamental research on this industrially important species. To the best of our knowledge, this is the first time a gene encoding for ATP-dependent NADH kinase (ATP:NADH 2'-phosphotransferase, EC 2.7.1.86) has been identif...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2008.08.008
更新日期:2009-01-01 00:00:00
abstract::The study was aimed at evaluating the extent of flux control exercised by the amino acid excretion step on the glutamate production flux in C. glutamicum 2262 strain that is induced for glutamate excretion by an upward temperature shift. Cells initially induced to excrete glutamate were cultivated at different control...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1006/mben.1999.0129
更新日期:1999-07-01 00:00:00