Abstract：
:A novel strategy to finely control a large metabolic flux by using a "metabolic transistor" approach was established. In this approach a small change in the level or availability of an essential component for the process is controlled by adding a competitive reaction that affects a precursor or an intermediate in its biosynthetic pathway. The change of the basal level of the essential component, considered as a base current in a transistor, has a large effect on the flux through the major pathway. In this way, the fine-tuning of a large flux can be accomplished. The "metabolic transistor" strategy was applied to control electron transfer chain function by manipulation of the quinone synthesis pathway in Escherichia coli. The achievement of a theoretical yield of lactate production under aerobic conditions via this strategy upon manipulation of the biosynthetic pathway of the key participant, ubiquinone-8 (Q8), in an E. coli strain provides an in vivo, genetically tunable means to control the activity of the electron transfer chain and manipulate the production of reduced products while limiting consumption of oxygen to a defined amount.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Wu H,Tuli L,Bennett GN,San KYdoi
10.1016/j.ymben.2015.01.002subject
Has Abstractpub_date
2015-03-01 00:00:00pages
159-168eissn
1096-7176issn
1096-7184pii
S1096-7176(15)00004-Xjournal_volume
28pub_type
杂志文章abstract：:Microbial fermentation conditions are dynamic, due to transcriptional induction, nutrient consumption, or changes to incubation conditions. In this study, 13C-metabolic flux analysis was used to characterize two violacein-producing E. coli strains with vastly different productivities, and to profile their metabolic ad...
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
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doi：10.1016/j.ymben.2018.04.010
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
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doi：10.1016/j.ymben.2020.04.003
更新日期：2020-07-01 00:00:00
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2019.07.012
更新日期：2020-01-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2011.11.003
更新日期：2012-01-01 00:00:00
abstract：:The promise of plants to serve as the green factories of the future is ever increasing. Plants have been used traditionally for construction, energy, food and feed. Bioactive compounds primarily derived from specialized plant metabolism continue to serve as important scaffold molecules for pharmaceutical drug producti...
journal_title：Metabolic engineering
pub_type： 杂志文章,评审
doi：10.1016/j.ymben.2014.11.005
更新日期：2015-01-01 00:00:00
abstract：:Two types of non-homologous beta-carotene ketolases (CrtW and CrtO) were previously described. We report improvement of a CrtO-type of beta-carotene ketolase for canthaxanthin production in a methylotrophic bacterium, Methylomonas sp. 16a, which could use the C1 substrate (methane or methanol) as sole carbon and energ...
journal_title：Metabolic engineering
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doi：10.1016/j.ymben.2007.05.004
更新日期：2007-07-01 00:00:00
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doi：10.1016/j.ymben.2014.11.013
更新日期：2015-03-01 00:00:00
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
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doi：10.1016/j.ymben.2011.03.003
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journal_title：Metabolic engineering
pub_type： 杂志文章,评审
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journal_title：Metabolic engineering
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2010.12.003
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journal_title：Metabolic engineering
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abstract：:Intense synthesis of proteins and chemicals in engineered microbes impose metabolic burden, frequently leading to reduced growth and heterogeneous cell population. Thus, the correct balance between growth and production is important. Such balance can be engineered through dynamic control of pathways, but few broadly a...
journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2020.03.012
更新日期：2020-09-01 00:00:00
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journal_title：Metabolic engineering
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doi：10.1016/j.ymben.2014.07.006
更新日期：2014-09-01 00:00:00
abstract：:Rational metabolic engineering methods are increasingly employed in designing the commercially viable processes for the production of chemicals relevant to pharmaceutical, biotechnology, and food and beverage industries. With the growing availability of omics data and of methodologies capable to integrate the availabl...
journal_title：Metabolic engineering
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doi：10.1016/j.ymben.2016.01.009
更新日期：2016-05-01 00:00:00
