Abstract:
:Metabolic engineering technology for industrial microorganisms is under development to create rational, more reliable, and more cost-effective approaches to strain improvement. Strain improvement is a critical component of the drug development process, yet the genetic basis for high production by industrial microorganisms is still a mystery. In this study, a search was begun for genetic modifications critical for high-level antibiotic production. The model system used was erythromycin production studied in the unicellular actinomycete, Aeromicrobium erythreum. A tagged-mutagenesis approach allowed reverse engineering of improved strains, revealing two genes, mutB and cobA, in the primary metabolic branch for methylmalonyl-CoA utilization. Knockouts in these genes created a permanent metabolic switch in the flow of methylmalonyl-CoA, from the primary branch into a secondary metabolic branch, driving erythromycin overproduction. The model provides insights into the regulation and evolution of secondary metabolism.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Reeves AR,Cernota WH,Brikun IA,Wesley RK,Weber JMdoi
10.1016/j.ymben.2004.03.003subject
Has Abstractpub_date
2004-10-01 00:00:00pages
300-12issue
4eissn
1096-7176issn
1096-7184pii
S1096717604000266journal_volume
6pub_type
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