abstract：:Human cytomegalovirus (HCMV) establishes life-long latent infection in hematopoietic progenitor cells and circulating monocytes in infected individuals. Myeloid differentiation coupled with immune dysregulation leads to viral reactivation, which can cause severe disease and mortality. Reactivation of latent virus requ...

journal_title：Journal of cell science

authors： Krishna BA,Wass AB,Dooley AL,O'Connor CM

更新日期：2021-01-25 00:00:00

abstract：:Tumour necrosis factor receptors (TNF-Rs) and their ligands, tumour necrosis factors, are highly conserved proteins described in all metazoan phyla. They function as inducers of extrinsic apoptotic signalling and facilitate inflammation, differentiation and cell survival. TNF-Rs use distinct adaptor molecules to activ...

journal_title：Journal of cell science

authors： Steichele M,Sauermann LS,König AC,Hauck S,Böttger A

更新日期：2021-01-25 00:00:00

abstract：:Crumbs (Crb) is an evolutionarily conserved transmembrane protein localised to the apical membrane of epithelial cells. Loss or mislocalisation of Crb is often associated with disruption of apicobasal cell polarity. crb mRNA is also apically enriched in epithelial cells, and, as shown here, accumulates in the oocyte o...

journal_title：Journal of cell science

authors： Bhagavatula S,Knust E

更新日期：2021-01-25 00:00:00

abstract：:In order to produce proteins essential for their propagation, many pathogenic human viruses, including SARS-CoV-2 the causative agent of COVID-19 respiratory disease, commandeer host biosynthetic machineries and mechanisms. Three major structural proteins, the spike, envelope and membrane proteins, are amongst several...

journal_title：Journal of cell science

authors： O'Keefe S,Roboti P,Duah KB,Zong G,Schneider H,Shi WQ,High S

更新日期：2021-01-19 00:00:00

abstract：:In invertebrates, UNC-45 regulates myosin stability and functions. Vertebrates have two distinct isoforms of the protein: UNC-45B, expressed in muscle cells only, and UNC-45A, expressed in all cells and implicated in regulating both non-muscle myosin II (NMII)- and microtubule (MT)-associated functions. Here, we show ...

journal_title：Journal of cell science

authors： Habicht J,Mooneyham A,Hoshino A,Shetty M,Zhang X,Emmings E,Yang Q,Coombes C,Gardner MK,Bazzaro M

更新日期：2021-01-08 00:00:00

abstract：:Dynamic assembly and remodeling of actin is critical for many cellular processes during development and stress adaptation. In filamentous fungi and budding yeast, actin cables align in a polarized manner along the mother-to-daughter cell axis, and are essential for the establishment and maintenance of polarity; moreov...

journal_title：Journal of cell science

authors： Xie Y,Miao Y

更新日期：2021-01-08 00:00:00

abstract：:Errors in mitotic chromosome segregation can lead to DNA damage and aneuploidy, both hallmarks of cancer. To achieve synchronous error-free segregation, mitotic chromosomes must align at the metaphase plate with stable amphitelic attachments to microtubules emanating from opposing spindle poles. The astrin-kinastrin (...

journal_title：Journal of cell science

authors： Geraghty Z,Barnard C,Uluocak P,Gruneberg U

更新日期：2021-01-08 00:00:00

abstract：:Borrelia burgdorferi is the causative agent of Lyme disease, a multisystemic disorder affecting primarily skin, joints and nervous system. Successful internalization and intracellular processing of borreliae by immune cells like macrophages is decisive for the outcome of a respective infection. Here, we use for the fi...

journal_title：Journal of cell science

authors： Klose M,Scheungrab M,Luckner M,Wanner G,Linder S

更新日期：2020-12-30 00:00:00

abstract：:Tissue development and homeostasis are controlled by mechanical cues. Perturbation of the mechanical equilibrium triggers restoration of mechanostasis through changes in cell behavior, while defects in these restorative mechanisms lead to mechanopathologies, for example, osteoporosis, myopathies, fibrosis or cardiovas...

journal_title：Journal of cell science

authors： Stassen OMJA,Ristori T,Sahlgren CM

更新日期：2020-12-21 00:00:00

abstract：:Start is the main decision point in the eukaryotic cell cycle at which cells commit to a new round of cell division. It involves the irreversible activation of a transcriptional programme through the inactivation of Start transcriptional repressors: the retinoblastoma family in mammals, or Whi5 and its recently identi...

journal_title：Journal of cell science

authors： Méndez E,Gomar-Alba M,Bañó MC,Mendoza M,Quilis I,Igual JC

更新日期：2020-12-21 00:00:00

abstract：:Activator of G-protein signaling 3 (AGS3, also known as GPSM1) regulates the trans-Golgi network. The AGS3 GoLoco motif binds to Gαi and thereby regulates the transport of proteins to the plasma membrane. Compaction of early embryos is based on the accumulation of E-cadherin (Cdh1) at cell-contacted membranes. However...

journal_title：Journal of cell science

authors： Nie ZW,Niu YJ,Zhou W,Zhou DJ,Kim JY,Cui XS

更新日期：2020-12-07 00:00:00

abstract：:Endocytosis of caveolae has previously been implicated in the repair of plasma membrane wounds. Here, we show that caveolin-1-deficient fibroblasts lacking caveolae upregulate a tubular endocytic pathway and have a reduced capacity to reseal after permeabilization with pore-forming toxins compared with wild-type cells...

journal_title：Journal of cell science

authors： Corrotte M,Cerasoli M,Maeda FY,Andrews NW

更新日期：2020-12-01 00:00:00

abstract：:Non-coding RNAs (ncRNAs) are nucleotide sequences that are known to assume regulatory roles previously thought to be reserved for proteins. Their functions include the regulation of protein activity and localization and the organization of subcellular structures. Sequencing studies have now identified thousands of ncR...

journal_title：Journal of cell science

authors： Bensaoud C,Martins LA,Aounallah H,Hackenberg M,Kotsyfakis M

更新日期：2020-11-05 00:00:00

abstract：:The core protease (CP) subcomplex of the 26S proteasome houses the proteolytic active sites and assumes a barrel shape comprised of four co-axially stacked heptameric rings formed by structurally related α- and β-subunits. CP biogenesis typically begins with the assembly of the α-ring, which then provides a template f...

journal_title：Journal of cell science

authors： Marshall RS,Gemperline DC,McLoughlin F,Book AJ,Hofmann K,Vierstra RD

更新日期：2020-11-03 00:00:00

abstract：:Autophagy requires the formation of membrane vesicles, known as autophagosomes, that engulf cellular cargoes and subsequently recruit lysosomal hydrolases for the degradation of their contents. A number of autophagy-related proteins act to mediate the de novo biogenesis of autophagosomes and vesicular trafficking even...

journal_title：Journal of cell science

authors： Gammoh N

更新日期：2020-10-30 00:00:00

abstract：:Sonic hedgehog (Shh) and its patched-smoothened receptor complex control a variety of functions in the developing central nervous system, such as neural cell proliferation and differentiation. Recently, Shh signaling components have been found to be expressed at the synaptic level in the postnatal brain, suggesting a ...

journal_title：Journal of cell science

authors： Delmotte Q,Hamze M,Medina I,Buhler E,Zhang J,Belgacem YH,Porcher C

更新日期：2020-10-23 00:00:00

abstract：:Most tissues harbor a substantial population of resident macrophages. Here, we elucidate a functional link between the Slc7a7 cationic amino acid transporter and tissue macrophages. We identified a mutant zebrafish devoid of microglia due to a mutation in the slc7a7 gene. We found that in Slc7a7-deficient larvae, macr...

journal_title：Journal of cell science

authors： Demy DL,Carrère M,Noche R,Tauzin M,Le Bris M,Baek C,Leshchiner I,Goessling W,Herbomel P

更新日期：2020-10-22 00:00:00

abstract：:Eukaryotic flagella undertake different beat types as necessary for different functions; for example, the Leishmania parasite flagellum undergoes a symmetric tip-to-base beat for forward swimming and an asymmetric base-to-tip beat to rotate the cell. In multi-ciliated tissues or organisms, the asymmetric beats are coo...

journal_title：Journal of cell science

authors： Wang Z,Beneke T,Gluenz E,Wheeler RJ

更新日期：2020-10-22 00:00:00

abstract：:The JAK/STAT pathway is an essential signalling cascade required for multiple processes during development and for adult homeostasis. A key question in understanding this pathway is how it is regulated in different cell contexts. Here, we have examined how endocytic processing contributes to signalling by the single c...

journal_title：Journal of cell science

authors： Moore R,Vogt K,Acosta-Martin AE,Shire P,Zeidler M,Smythe E

更新日期：2020-10-12 00:00:00

abstract：:Besides regulating glucose levels, insulin has been reported to participate actively in many other functions, including modulating inflammatory reactions. In this study we investigated how topical insulin application would affect the diabetic wound healing process. We found that the excessive expression of insulin-deg...

journal_title：Journal of cell science

authors： Yang P,Wang X,Wang D,Shi Y,Zhang M,Yu T,Liu D,Gao M,Zhang X,Liu Y

更新日期：2020-10-12 00:00:00

abstract：:Current methodologies for targeting the mitochondrial genome for research and/or therapy development in mitochondrial diseases are restricted by practical limitations and technical inflexibility. A molecular toolbox for CRISPR-mediated mitochondrial genome editing is desirable, as this could enable targeting of mtDNA ...

journal_title：Journal of cell science

authors： Antón Z,Mullally G,Ford HC,van der Kamp MW,Szczelkun MD,Lane JD

更新日期：2020-09-16 00:00:00

abstract：:p63 (also known as TP63) is a transcription factor of the p53 family, along with p73. Multiple isoforms of p63 have been discovered and these have diverse functions encompassing a wide array of cell biology. p63 isoforms are implicated in lineage specification, proliferative potential, differentiation, cell death and ...

journal_title：Journal of cell science

authors： Fisher ML,Balinth S,Mills AA

更新日期：2020-09-11 00:00:00

abstract：:Autophagy is fundamental for cell and organismal health. Two types of autophagy are conserved in eukaryotes: macroautophagy and microautophagy. During macroautophagy, autophagosomes deliver cytoplasmic constituents to endosomes or lysosomes, whereas during microautophagy lytic organelles take up cytoplasm directly. Wh...

journal_title：Journal of cell science

authors： Schuck S

更新日期：2020-09-09 00:00:00

abstract：:Cells experience mechanical stresses in different physiological and pathological settings. Clathrin-coated structures (CCSs) are sensitive to such perturbations in a way that often results in a mechanical impairment of endocytic budding. Compressive stress is a mechanical perturbation that leads to increased membrane ...

journal_title：Journal of cell science

authors： Baschieri F,Le Devedec D,Tettarasar S,Elkhatib N,Montagnac G

更新日期：2020-09-01 00:00:00

abstract：:During cell division, misaligned chromosomes are captured and aligned by motors before their segregation. The CENP-E motor is recruited to polar unattached kinetochores to facilitate chromosome alignment. The spindle checkpoint protein BubR1 (also known as BUB1B) has been reported as a CENP-E interacting partner, but ...

journal_title：Journal of cell science

authors： Legal T,Hayward D,Gluszek-Kustusz A,Blackburn EA,Spanos C,Rappsilber J,Gruneberg U,Welburn JPI

更新日期：2020-08-25 00:00:00

abstract：:Long noncoding RNAs (lncRNAs) have emerged as important regulators of cancer progression. Abnormal sialylation leads to renal cell carcinoma (RCC) malignancy. However, the mechanism by which the lncRNA maternally expressed gene 3 (MEG3) mediates RCC progression by regulating ST3Gal1 transcription and EGFR sialylation ...

journal_title：Journal of cell science

authors： Gong A,Zhao X,Pan Y,Qi Y,Li S,Huang Y,Guo Y,Qi X,Zheng W,Jia L

更新日期：2020-08-25 00:00:00

abstract：:BMP2 stimulates bone formation and signals preferably through BMP receptor (BMPR) 1A, whereas GDF5 is a cartilage inducer and signals preferably through BMPR1B. Consequently, BMPR1A and BMPR1B are believed to be involved in bone and cartilage formation, respectively. However, their function is not yet fully clarified....

journal_title：Journal of cell science

authors： Mang T,Kleinschmidt-Doerr K,Ploeger F,Schoenemann A,Lindemann S,Gigout A

更新日期：2020-08-21 00:00:00

abstract：:CENP-B binds to CENP-B boxes on centromeric satellite DNAs (known as alphoid DNA in humans). CENP-B maintains kinetochore function through interactions with CENP-A nucleosomes and CENP-C. CENP-B binding to transfected alphoid DNA can induce de novo CENP-A assembly, functional centromere and kinetochore formation, and ...

journal_title：Journal of cell science

authors： Otake K,Ohzeki JI,Shono N,Kugou K,Okazaki K,Nagase T,Yamakawa H,Kouprina N,Larionov V,Kimura H,Earnshaw WC,Masumoto H

更新日期：2020-08-11 00:00:00

abstract：:Clathrin-mediated endocytosis (CME) is a crucial cellular process implicated in many aspects of plant growth, development, intra- and intercellular signaling, nutrient uptake and pathogen defense. Despite these significant roles, little is known about the precise molecular details of how CME functions in planta To fac...

journal_title：Journal of cell science

authors： Johnson A,Gnyliukh N,Kaufmann WA,Narasimhan M,Vert G,Bednarek SY,Friml J

更新日期：2020-08-06 00:00:00

abstract：:Cystogenesis is a morphological consequence of numerous genetic diseases of the epithelium. In the kidney, the pathogenic mechanisms underlying the program of altered cell and tubule morphology are obscured by secondary effects of cyst expansion. Here, we developed a new 3D tubuloid system to isolate the rapid changes...

journal_title：Journal of cell science

authors： Dixon EE,Maxim DS,Halperin Kuhns VL,Lane-Harris AC,Outeda P,Ewald AJ,Watnick TJ,Welling PA,Woodward OM

更新日期：2020-07-16 00:00:00

abstract：:The canonical Wnt/β-catenin signaling pathway regulates cell proliferation in development and adult tissue homeostasis. Dysregulated signaling contributes to human diseases, in particular cancer. Growing evidence suggests a role for clathrin and/or endocytosis in the regulation of this pathway, but conflicting results...

journal_title：Journal of cell science

authors： Munthe E,Raiborg C,Stenmark H,Wenzel EM

更新日期：2020-07-09 00:00:00

abstract：:The Cep63-Cep152 complex located at the mother centriole recruits Plk4 to initiate centriole biogenesis. How the complex is targeted to mother centrioles, however, is unclear. In this study, we show that Cep57 and its paralog, Cep57l1, colocalize with Cep63 and Cep152 at the proximal end of mother centrioles in both c...

journal_title：Journal of cell science

authors： Zhao H,Yang S,Chen Q,Duan X,Li G,Huang Q,Zhu X,Yan X

更新日期：2020-07-03 00:00:00

abstract：:Osteoclasts are giant multinucleated myeloid cells specialized for bone resorption, which is essential for the preservation of bone health throughout life. The activity of osteoclasts relies on the typical organization of osteoclast cytoskeleton components into a highly complex structure comprising actin, microtubules...

journal_title：Journal of cell science

authors： Blangy A,Bompard G,Guerit D,Marie P,Maurin J,Morel A,Vives V

更新日期：2020-07-01 00:00:00

abstract：:Intracellular pools of the heterotrimeric G-protein α-subunit Gαi3 (encoded by GNAI3) have been shown to promote growth factor signaling, while at the same time inhibiting the activation of JNK and autophagic signaling following nutrient starvation. The precise molecular mechanisms linking Gαi3 to both stress and grow...

journal_title：Journal of cell science

authors： Bastin G,Dissanayake K,Langburt D,Tam ALC,Lee SH,Lachhar K,Heximer SP

更新日期：2020-06-24 00:00:00

abstract：:The molecular motor dynein is essential for mitotic spindle orientation, which defines the axis of cell division. The light intermediate chain subunits, LIC1 and LIC2, define biochemically and functionally distinct vertebrate dynein complexes, with LIC2-dynein playing a crucial role in ensuring spindle orientation. We...

journal_title：Journal of cell science

authors： Sharma A,Dagar S,Mylavarapu SVS

更新日期：2020-06-23 00:00:00

abstract：:RIF1 controls both DNA replication timing and the DNA double-strand break (DSB) repair pathway to maintain genome integrity. However, it remains unclear how RIF1 links these two processes following exposure to ionizing radiation (IR). Here, we show that inhibition of homologous recombination repair (HRR) by RIF1 occur...

journal_title：Journal of cell science

authors： Saito Y,Kobayashi J,Kanemaki MT,Komatsu K

更新日期：2020-06-22 00:00:00

abstract：:Activity of AURKA is controlled through multiple mechanisms including phosphorylation, ubiquitin-mediated degradation and allosteric interaction with TPX2. Activity peaks at mitosis, before AURKA is degraded during and after mitotic exit in a process strictly dependent on the APC/C coactivator FZR1. We used FZR1 knock...

journal_title：Journal of cell science

authors： Abdelbaki A,Akman HB,Poteau M,Grant R,Gavet O,Guarguaglini G,Lindon C

更新日期：2020-06-16 00:00:00

abstract：:Osh6 and Osh7 are lipid transfer proteins (LTPs) that move phosphatidylserine (PS) from the endoplasmic reticulum (ER) to the plasma membrane (PM). High PS levels at the PM are key for many cellular functions. Intriguingly, Osh6 and Osh7 localize to ER-PM contact sites, although they lack membrane-targeting motifs, in...

journal_title：Journal of cell science

authors： D'Ambrosio JM,Albanèse V,Lipp NF,Fleuriot L,Debayle D,Drin G,Čopič A

更新日期：2020-06-04 00:00:00

abstract：:The specification of organs, tissues and cell types results from cell fate restrictions enacted by nuclear transcription factors under the control of conserved signaling pathways. The progenitor epithelium of the Drosophila compound eye, the eye imaginal disc, is a premier model for the study of such processes. Early ...

journal_title：Journal of cell science

authors： Neal SJ,Zhou Q,Pignoni F

更新日期：2020-05-26 00:00:00

abstract：:Stressed cells downregulate translation initiation and assemble membrane-less foci termed stress granules (SGs). Although SGs have been extensively characterized in cultured cells, the existence of such structures in stressed adult stem cell pools remains poorly characterized. Here, we report that the Drosophila ortho...

journal_title：Journal of cell science

authors： Buddika K,Ariyapala IS,Hazuga MA,Riffert D,Sokol NS

更新日期：2020-05-18 00:00:00