AGS3-dependent trans-Golgi network membrane trafficking is essential for compaction in mouse embryos.

abstract：:The process of regulated exocytosis is defined by the Ca2+-triggered fusion of two apposed membranes, enabling the release of vesicular contents. This fusion step involves a number of energetically complex steps and requires both protein and lipid membrane components. The role of cholesterol has been investigated usin...

journal_title：Journal of cell science

authors： Churchward MA,Rogasevskaia T,Höfgen J,Bau J,Coorssen JR

更新日期：2005-10-15 00:00:00

abstract：:An acrosome reaction was induced in ejaculated ram spermatozoa by treatment with calcium and the ionophore A23187. Samples were fixed at different times after initiation of induction, and the morphological changes within the head membranes that took place as exocytosis occurred were studied in freeze-fracture replicas...

journal_title：Journal of cell science

authors： Fléchon JE,Harrison RA,Fléchon B,Escaig J

更新日期：1986-03-01 00:00:00

abstract：:Neurite outgrowth is known as a slow (days) process occurring in nerve cells and neurons during neurotrophin treatment and upon transfer to culture, respectively. Using Y27632, a drug that induces activation of Rac1, a downstream step of the neurotrophin signaling cascade, we have identified a new form of outgrowth, w...

journal_title：Journal of cell science

authors： Racchetti G,Lorusso A,Schulte C,Gavello D,Carabelli V,D'Alessandro R,Meldolesi J

更新日期：2010-01-15 00:00:00

abstract：:Fe-S clusters act as co-factors of proteins with diverse functions, for example, in DNA repair. Downregulation of the cytosolic iron-sulfur protein assembly (CIA) machinery promotes genomic instability through the inactivation of multiple DNA repair pathways. Furthermore, CIA deficiencies are associated with so far un...

journal_title：Journal of cell science

authors： Ben-Shimon L,Paul VD,David-Kadoch G,Volpe M,Stümpfig M,Bill E,Mühlenhoff U,Lill R,Ben-Aroya S

更新日期：2018-06-27 00:00:00

abstract：:Cytoplasmic dynein-2 is a motor protein complex that drives the movement of cargoes along microtubules within cilia, facilitating the assembly of these organelles on the surface of nearly all mammalian cells. Dynein-2 is crucial for ciliary function, as evidenced by deleterious mutations in patients with skeletal abno...

journal_title：Journal of cell science

authors： Vuolo L,Stevenson NL,Mukhopadhyay AG,Roberts AJ,Stephens DJ

更新日期：2020-03-30 00:00:00

abstract：:The Na(+)/H(+) exchanger NHE1 is involved in intracellular pH homeostasis and cell volume regulation and accumulates with actin in the lamellipodia of fibroblasts. In order to determine the role of NHE1 following epithelial transformation and the acquisition of motile and invasive properties, we studied NHE1 expressio...

journal_title：Journal of cell science

authors： Lagana A,Vadnais J,Le PU,Nguyen TN,Laprade R,Nabi IR,Noël J

更新日期：2000-10-01 00:00:00

abstract：:The dynamics of cell adhesion sites control cell morphology and motility. Adhesion-site turnover is thought to depend on the local availability of the acidic phospholipid phosphatidylinositol-4,5-bisphosphate (PIP(2)). PIP(2) can bind to many cell adhesion proteins such as vinculin and talin, but the consequences of t...

journal_title：Journal of cell science

authors： Chandrasekar I,Stradal TE,Holt MR,Entschladen F,Jockusch BM,Ziegler WH

更新日期：2005-04-01 00:00:00

abstract：:Collective cellular migration within the epithelial layer impacts upon development, wound healing and cancer invasion, but remains poorly understood. Prevailing conceptual frameworks tend to focus on the isolated role of each particular underlying factor - taken one at a time or at most a few at a time - and thus migh...

journal_title：Journal of cell science

authors： Park JA,Atia L,Mitchel JA,Fredberg JJ,Butler JP

更新日期：2016-09-15 00:00:00

abstract：:Myosin VI is an actin-based retrograde motor protein that plays a crucial role in both endocytic and secretory membrane trafficking pathways. Myosin VI's targeting to and function in these intracellular pathways is mediated by a number of specific binding partners. In this paper we have identified a new myosin-VI-bind...

journal_title：Journal of cell science

authors： Chibalina MV,Seaman MN,Miller CC,Kendrick-Jones J,Buss F

更新日期：2007-12-15 00:00:00

abstract：:Cadherins are transmembrane glycoproteins involved in Ca2+-dependent cell-cell adhesion. Using L cells expressing one of three functional E-cadherin constructs, the wild-type, a chimeric molecule with alpha-catenin (EalphaC), and a tail-less one, we determined the effect of v-Src expression on E-cadherin-mediated adhe...

journal_title：Journal of cell science

authors： Ozawa M,Ohkubo T

更新日期：2001-02-01 00:00:00

abstract：:Prion diseases are fatal transmissible neurodegenerative disorders characterized by the accumulation of an abnormally folded isoform of the cellular prion protein (PrP(C)) denoted PrP(Sc). Recently, wild-type and pathogenic PrP mutants have been shown to be degraded by the endoplasmic reticulum-associated degradation ...

journal_title：Journal of cell science

authors： Mangé A,Crozet C,Lehmann S,Béranger F

更新日期：2004-05-01 00:00:00

abstract：:The cytoplasmic pH of growing pollen tubes of Lilium longiflorum Thunb. was measured using the pH-sensitive fluorescent dye 2',7'-bis-(carboxyethyl)-5(6')-carboxyfl uorescein and confocal fluorescence ratio imaging. The average cytoplasmic pH in the clear zone of the pollen tube tip was pH 7.11, and no consistent pH g...

journal_title：Journal of cell science

authors： Fricker MD,White NS,Obermeyer G

更新日期：1997-08-01 00:00:00

abstract：:Transcripts containing expanded CNG repeats, which are found in several neuromuscular diseases, are not exported from the nucleus and aggregate as ribonuclear inclusions by an unknown mechanism. Using the MS2-GFP system, which tethers fluorescent proteins to a specific mRNA, we followed the dynamics of single CUG-repe...

journal_title：Journal of cell science

authors： Querido E,Gallardo F,Beaudoin M,Ménard C,Chartrand P

更新日期：2011-05-15 00:00:00

abstract：:Ran is an abundant GTPase of the Ras superfamily that is highly conserved in eukaryotes. In interphase cells, Ran is mainly nuclear and thought to be predominantly GTP-bound, but it is also present in the cytoplasm, probably GDP-bound. This asymmetric distribution plays an important role in directing nucleocytoplasmic...

journal_title：Journal of cell science

authors： Zhang C,Hughes M,Clarke PR

更新日期：1999-07-01 00:00:00

abstract：:We have isolated mini-titin from the nematodes Ascaris lumbricoides and Caenorhabditis elegans under native conditions using a modification in the procedure to prepare this protein from insect muscle. The proteins have an apparent molecular weight of 600,000 and appear in oriented specimens as flexible thin rods with ...

journal_title：Journal of cell science

authors： Nave R,Fürst D,Vinkemeier U,Weber K

更新日期：1991-04-01 00:00:00

abstract：:The developmental stage at which Dictyostelium discoideum strain v12/M2 cells was inhibited in resuming cell division was monitored by a Coulter counter. Neither vegetative nor pre-aggregating v12/M2 amoebae used as inocula in liquid growth cultures exhibited a delay in recommencing cell division. Inocula prepared fro...

journal_title：Journal of cell science

authors： Kopachik WJ,Gregg JH

更新日期：1978-02-01 00:00:00

abstract：:Keratins 8 and 18 (K8 and K18) are regulated by site-specific phosphorylation in response to multiple stresses. We examined the effect and regulation of hyposmotic stress on keratin phosphorylation. K8 phospho-Ser431 (Ser431-P) becomes dephosphorylated in HT29 cells, but hyperphosphorylated on other K8 but not K18 sit...

journal_title：Journal of cell science

authors： Tao GZ,Toivola DM,Zhou Q,Strnad P,Xu B,Michie SA,Omary MB

更新日期：2006-04-01 00:00:00

abstract：:A line of null mice has been produced which fails to express the transmembrane chondroitin sulfate proteoglycan NG2. Homozygous NG2 null mice do not exhibit gross phenotypic differences from wild-type mice, suggesting that detailed analyses are required to detect subtle alterations caused by the absence of NG2. Accord...

journal_title：Journal of cell science

authors： Grako KA,Ochiya T,Barritt D,Nishiyama A,Stallcup WB

更新日期：1999-03-01 00:00:00

abstract：:RAS isoforms have been proposed to exhibit differing biological outputs due to differences in their relative occupancy of cellular organelles and signalling microdomains. The membrane binding and targeting motifs of RAS are encoded by the C-terminal hypervariable region (HVR), and the precise localisation depends upon...

journal_title：Journal of cell science

authors： Laude AJ,Prior IA

更新日期：2008-02-15 00:00:00

abstract：:Arf GTPases regulate both the morphological and protein sorting events that are essential for membrane trafficking. Guanine nucleotide exchange factors (GEFs) specific for Arf proteins determine when and where Arf GTPases will be activated in cells. The yeast Gea2p Arf GEF is a member of an evolutionarily conserved fa...

journal_title：Journal of cell science

authors： Chantalat S,Park SK,Hua Z,Liu K,Gobin R,Peyroche A,Rambourg A,Graham TR,Jackson CL

更新日期：2004-02-15 00:00:00

abstract：:Intracellular membrane fusion occurs with exquisite coordination and specificity. Each fusion event requires three basic components: Rab-GTPases organize the fusion site; SNARE proteins act during fusion; and N-ethylmaleimide-sensitive factor (NSF) plus its cofactor alpha-SNAP are required for recycling or activation ...

journal_title：Journal of cell science

authors： Ungermann C,Langosch D

更新日期：2005-09-01 00:00:00

abstract：:SH4 domains provide bipartite membrane-targeting signals for oncogenic Src family kinases. Here we report the induction of non-apoptotic plasma membrane (PM) blebbing as a novel and conserved activity of SH4 domains derived from the prototypic Src kinases Src, Fyn, Yes and Lck as well as the HASPB protein of Leishmani...

journal_title：Journal of cell science

authors： Tournaviti S,Hannemann S,Terjung S,Kitzing TM,Stegmayer C,Ritzerfeld J,Walther P,Grosse R,Nickel W,Fackler OT

更新日期：2007-11-01 00:00:00

abstract：:TRF1 is a key component of the telomere-capping complex and binds double-strand telomeric DNA as homodimers. So far, it is not clear whether TRF1 dimerization coincides with its telomere binding or is actively controlled before it binds the telomere, and in the latter case, how this event might affect its telomere ass...

journal_title：Journal of cell science

authors： Meng L,Hsu JK,Zhu Q,Lin T,Tsai RY

更新日期：2011-11-01 00:00:00

abstract：:Accumulation of adipocytes and collagen type-I-producing cells (fibrosis) is observed in muscular dystrophies. The origin of these cells had been largely unknown, but recently we identified mesenchymal progenitors positive for platelet-derived growth factor receptor alpha (PDGFRα) as the origin of adipocytes in skelet...

journal_title：Journal of cell science

authors： Uezumi A,Ito T,Morikawa D,Shimizu N,Yoneda T,Segawa M,Yamaguchi M,Ogawa R,Matev MM,Miyagoe-Suzuki Y,Takeda S,Tsujikawa K,Tsuchida K,Yamamoto H,Fukada S

更新日期：2011-11-01 00:00:00

abstract：:Asymmetric cell division can produce daughter cells with different developmental fates and is often accompanied by a difference in cell size. A number of recent genetic and in vivo imaging studies in Drosophila and Caenorhabditis elegans have begun to elucidate the mechanisms underlying the rearrangements of the cytos...

journal_title：Journal of cell science

authors： Kaltschmidt JA,Brand AH

更新日期：2002-06-01 00:00:00

abstract：:Clathrin heavy chain is the structural component of the clathrin triskelion, but unique functions for the two distinct and highly conserved clathrin light chains (CLCa and CLCb, also known as CLTA and CLTB, respectively) have been elusive. Here, we show that following detachment and replating, CLCa is uniquely respons...

journal_title：Journal of cell science

authors： Tsygankova OM,Keen JH

更新日期：2019-05-15 00:00:00

abstract：:Removal of interleukin-3 from murine IC.DP pre-mast cells results in irreversible commitment to apoptosis within 18 hours. To identify early events necessary for the engagement of apoptosis we examined the regulation of intracellular pH (pH(i)). IC.DP cells acidified 2 hours after removal of interleukin-3 (before disc...

journal_title：Journal of cell science

authors： Chen Q,Benson RS,Whetton AD,Brant SR,Donowitz M,Montrose MH,Dive C,Watson AJ

更新日期：1997-02-01 00:00:00

abstract：:Successful cytokinesis requires proper assembly of the contractile actomyosin ring, its stable positioning on the cell surface and proper constriction. Over the years, many of the key molecular components and regulators of the assembly and positioning of the actomyosin ring have been elucidated. Here we show that cell...

journal_title：Journal of cell science

authors： Mishra M,Huang Y,Srivastava P,Srinivasan R,Sevugan M,Shlomovitz R,Gov N,Rao M,Balasubramanian M

更新日期：2012-08-15 00:00:00

abstract：:Axonemal dyneins are multisubunit molecular motors that provide the driving force for flagellar motility. Dynein light chain 1 (LC1) has been well studied in Chlamydomonas reinhardtii and is unique among all dynein components as the only protein known to bind directly to the catalytic motor domain of the dynein heavy ...

journal_title：Journal of cell science

authors： Baron DM,Kabututu ZP,Hill KL

更新日期：2007-05-01 00:00:00

abstract：:Elementary Ca(2+) signals, such as "Ca(2+) puffs", which arise from the activation of inositol 1,4,5-trisphosphate receptors, are building blocks for local and global Ca(2+) signalling. We characterized Ca(2+) puffs in six cell types that expressed differing ratios of the three inositol 1,4,5-trisphosphate receptor is...

journal_title：Journal of cell science

authors： Tovey SC,de Smet P,Lipp P,Thomas D,Young KW,Missiaen L,De Smedt H,Parys JB,Berridge MJ,Thuring J,Holmes A,Bootman MD

更新日期：2001-11-01 00:00:00