SH4-domain-induced plasma membrane dynamization promotes bleb-associated cell motility.

abstract：:We report the first direct analysis of the endogenous β-catenin phosphorylation activity in colon cancer SW480 cells. By comparing parental SW480 cells that harbor a typical truncated adenomatous polyposis coli (APC) form, cells expressing full-length APC and APC-depleted cells, we provide the formal demonstration tha...

journal_title：Journal of cell science

authors： Wang L,Liu X,Gusev E,Wang C,Fagotto F

更新日期：2014-04-15 00:00:00

abstract：:The extracellular matrix molecule tenascin-R (TN-R) and the proteoglycans of the lectican family show an overlapping distribution in the developing brain, have been implicated in similar cellular processes and form a complex network of interactions. Previously, we have demonstrated that TN-R induces microprocesses alo...

journal_title：Journal of cell science

authors： Zacharias U,Rauch U

更新日期：2006-08-15 00:00:00

abstract：:Although the anti-apoptotic activity of Bcl-2 has been extensively studied, its mode of action is still incompletely understood. In the nematode Caenorhabditis elegans, 131 of 1090 somatic cells undergo programmed cell death during development. Transgenic expression of human Bcl-2 reduced cell death during nematode de...

journal_title：Journal of cell science

authors： Jabbour AM,Puryer MA,Yu JY,Lithgow T,Riffkin CD,Ashley DM,Vaux DL,Ekert PG,Hawkins CJ

更新日期：2006-06-15 00:00:00

abstract：:Monoclonal mouse hybridoma antibodies were obtained for secreted cellular fibronectin (cFn) from A8387 fibrosarcoma cells. One of them, 52-DH1 (DH), reacted exclusively with cFns but not with plasma Fns (pFns) in immunoblotting and solid-phase EIA. The DH antibody also recognized thermolysin cFn fragments and beta-gal...

journal_title：Journal of cell science

authors： Vartio T,Laitinen L,Närvänen O,Cutolo M,Thornell LE,Zardi L,Virtanen I

更新日期：1987-11-01 00:00:00

abstract：:Transglutaminases (TGases) are enzymes which catalyze cross-link formation between glutamine residues and lysine residues in substrate proteins. In the present study, we report for the first time that a representative enzyme, blood coagulation factor XIIIa (FXIIIa), is capable of mediating adhesion of various cells. W...

journal_title：Journal of cell science

authors： Ueki S,Takagi J,Saito Y

更新日期：1996-11-01 00:00:00

abstract：:Podosomes are actin-rich adhesion and invasion structures. Especially in macrophages, podosomes exist in two subpopulations, large precursors at the cell periphery and smaller podosomes (successors) in the cell interior. To date, the mechanisms that differentially regulate these subpopulations are largely unknown. Her...

journal_title：Journal of cell science

authors： Bhuwania R,Cornfine S,Fang Z,Krüger M,Luna EJ,Linder S

更新日期：2012-05-01 00:00:00

abstract：:A cDNA encoding rat CaBP1 has been isolated and sequenced. The deduced polypeptide chain consists of 440 amino acids including two internal thioredoxin-like domains and a C-terminal KDEL retention/retrieval signal. Regarding the high degree of identity to the hamster protein P5, CaBP1 is considered to be the homologou...

journal_title：Journal of cell science

authors： Füllekrug J,Sönnichsen B,Wünsch U,Arseven K,Nguyen Van P,Söling HD,Mieskes G

更新日期：1994-10-01 00:00:00

abstract：:In situ hybridization has become a powerful technique for dissecting nuclear structure. By localizing nucleic acids with high precision, it is possible to infer the native structure of chromosomes, replication factories and transcript processing complexes. To increase the value of this technique, we have established t...

journal_title：Journal of cell science

authors： Gunawardena S,Heddle E,Rykowski MC

更新日期：1995-05-01 00:00:00

abstract：:Eukaryotic cell cycles are controlled by the activities of cyclin-dependent kinases (cdks). The major cdk in budding yeast, Saccharomyces cerevisiae, is Cdc28p. Activation of Cdc28p requires phosphorylation on threonine 169 and binding to a cyclin. Thr-169 is phosphorylated by the cdk-activating kinase (CAK), Cak1p, w...

journal_title：Journal of cell science

authors： Kaldis P,Pitluk ZW,Bany IA,Enke DA,Wagner M,Winter E,Solomon MJ

更新日期：1998-12-18 00:00:00

abstract：:The synaptonemal complex is an elaborate meiosis-specific supramolecular protein assembly that promotes chromosome synapsis and meiotic recombination. We inactivated the meiosis-specific gene Tex12 and found that TEX12 is essential for progression of meiosis in both male and female germ cells. Structural analysis of t...

journal_title：Journal of cell science

authors： Hamer G,Wang H,Bolcun-Filas E,Cooke HJ,Benavente R,Höög C

更新日期：2008-08-01 00:00:00

abstract：:The growth of two human diploid skin fibroblast cell lines, originally grown in medium supplemented with foetal bovine serum and later adapted to medium supplemented with newborn bovine, bovine calf or horse serum, has been studied. Prolonged generation times increased cell volumes and decreased plating efficiencies w...

journal_title：Journal of cell science

authors： Zamansky GB,Arundel C,Nagasawa H,Little JB

更新日期：1983-05-01 00:00:00

abstract：:The protein import machinery of the peroxisome consists of many proteins, collectively called the peroxins. By applying the split-ubiquitin technique we systematically tested the pair-wise interactions between the Nub- and Cub-labeled peroxins for the first time in the living cells of the yeast Saccharomyces cerevisia...

journal_title：Journal of cell science

authors： Eckert JH,Johnsson N

更新日期：2003-09-01 00:00:00

abstract：:Plastids in plants and algae evolved from the endosymbiotic integration of a cyanobacterium by a heterotrophic eukaryote. New plastids can only emerge through fission; thus, the synchronization of bacterial division with the cell cycle of the eukaryotic host was vital to the origin of phototrophic eukaryotes. Most of ...

journal_title：Journal of cell science

authors： de Vries J,Gould SB

更新日期：2018-01-29 00:00:00

abstract：:When cultured cerebellar macroneurons develop attached to a laminin-containing substrate or after the acute addition of laminin to the tissue culture medium, there is an acceleration in the rate and extent of axonal elongation. Furthermore, laminin is capable of inducing axonal formation and microtubule stabilization ...

journal_title：Journal of cell science

authors： DiTella MC,Feiguin F,Carri N,Kosik KS,Cáceres A

更新日期：1996-02-01 00:00:00

abstract：:Delayed wound healing caused by inefficient re-epithelialization underlines chronic skin lesions such as those found in diabetes. The dioxin receptor (AhR) modulates cell plasticity and migration and its activation by occupational polycyclic aromatic hydrocarbons (PAHs) results in severe skin lesions such as contact h...

journal_title：Journal of cell science

authors： Carvajal-Gonzalez JM,Roman AC,Cerezo-Guisado MI,Rico-Leo EM,Martin-Partido G,Fernandez-Salguero PM

更新日期：2009-06-01 00:00:00

abstract：:Borrelia burgdorferi is the causative agent of Lyme disease, a multisystemic disorder affecting primarily skin, joints and nervous system. Successful internalization and intracellular processing of borreliae by immune cells like macrophages is decisive for the outcome of a respective infection. Here, we use for the fi...

journal_title：Journal of cell science

authors： Klose M,Scheungrab M,Luckner M,Wanner G,Linder S

更新日期：2020-12-30 00:00:00

abstract：:CDC6, a replication licensing protein, is partially exported to the cytoplasm in human cells through phosphorylation by Cdk during S phase, but a significant proportion remains in the nucleus. We report here that human CDC6 physically interacts with ATR, a crucial checkpoint kinase, in a manner that is stimulated by p...

journal_title：Journal of cell science

authors： Yoshida K,Sugimoto N,Iwahori S,Yugawa T,Narisawa-Saito M,Kiyono T,Fujita M

更新日期：2010-01-15 00:00:00

abstract：:To understand the mechanism involved in the apical sorting of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) we fused to the C-terminus of GFP the GPI-anchor-attachment signal of the folate receptor (FR) or of the prion protein (PrP), two native GPI-anchored proteins that are sorted apically or basolat...

journal_title：Journal of cell science

authors： Paladino S,Lebreton S,Tivodar S,Campana V,Tempre R,Zurzolo C

更新日期：2008-12-15 00:00:00

abstract：:C-CAM (Cell-CAM 105) is a cell surface glycoprotein that is involved in cell-cell adhesion of rat hepatocytes in vitro. To elucidate the adhesion mechanism the binding properties of purified C-CAM were investigated. Using proteins immobilized on nitrocellulose it was found that radiolabeled C-CAM bound to C-CAM but no...

journal_title：Journal of cell science

authors： Tingström A,Blikstad I,Aurivillius M,Obrink B

更新日期：1990-05-01 00:00:00

abstract：:Cryo-ultramicrotomy and X-ray microanalysis were used to study the elemental composition of HeLa S3 cells. Quantitation was achieved by reference to elemental standards of known concentration made up in 25% gelatin. Analysis of standards showed linear calibration for each of the elements studied: Na, P, S, Cl, K. Stan...

journal_title：Journal of cell science

authors： Warley A,Stephen J,Hockaday A,Appleton TC

更新日期：1983-03-01 00:00:00

abstract：:Cells that have been pre-exposed to thermal stress can acquire a transient resistance against the killing effect of a subsequent thermal stress. The cause for this phenomenon, called thermotolerance, seems to be an enhanced resistance of proteins against thermal denaturation and aggregation. This resistance can be exp...

journal_title：Journal of cell science

authors： Kampinga HH

更新日期：1993-01-01 00:00:00

abstract：:Evidence from various systems suggests that either asters or the midzone of the mitotic spindle are the predominant determinants of cleavage plane position. Disrupting spindle midzone formation in the one-cell Caenorhabditis elegans embryo, such as by using mutants of the centralspindlin component ZEN-4, prevents comp...

journal_title：Journal of cell science

authors： Verbrugghe KJ,White JG

更新日期：2007-05-15 00:00:00

abstract：:The type II transmembrane protein CD98, best known as the heavy chain of the heterodimeric amino acid transporters (HAT), is required for the surface expression and basolateral localization of this transporter complex in polarized epithelial cells. CD98 also interacts with beta1 integrins resulting in an increase in t...

journal_title：Journal of cell science

authors： Cai S,Bulus N,Fonseca-Siesser PM,Chen D,Hanks SK,Pozzi A,Zent R

更新日期：2005-03-01 00:00:00

abstract：:During meiosis, cohesion between sister chromatids is required for normal levels of homologous recombination, maintenance of chiasmata and accurate chromosome segregation during both divisions. In Drosophila, null mutations in the ord gene abolish meiotic cohesion, although how ORD protein promotes cohesion has remain...

journal_title：Journal of cell science

authors： Khetani RS,Bickel SE

更新日期：2007-09-01 00:00:00

abstract：:New concepts in cell organization emerged in a medieval castle during a snowy week in January 2017 in the middle of the Austrian Alps. The occasion was the 10th Annaberg EMBO workshop in Goldegg am See; organized by Gabriele Seethaler, Catherine Rabouille and Marino Zerial. There were 95 participants, including many w...

journal_title：Journal of cell science

authors： Teis D,Kukulski W

更新日期：2017-07-15 00:00:00

abstract：:Skeletal and heart muscle-specific variant of the α subunit of nascent polypeptide associated complex (skNAC; encoded by NACA) is exclusively found in striated muscle cells. Its function, however, is largely unknown. Previous reports have demonstrated that skNAC binds to m-Bop/Smyd1, a multi-functional protein that re...

journal_title：Journal of cell science

authors： Berkholz J,Michalick L,Munz B

更新日期：2014-09-01 00:00:00

abstract：:By an improved freeze-fracture device, a number of double replicas were obtained from the rod disk membranes of the carp retina for investigation of complementary, continuing, and transitional relationships between fracture faces. Some separate particles found along the smooth edge bordering on the adjacent interdisk ...

journal_title：Journal of cell science

authors： Tonosaki A,Washioka H,Yamasaki M,Mizoguchi J

更新日期：1980-04-01 00:00:00

abstract：:MACF (microtubule actin cross-linking factor) is a large, 608-kDa protein that can associate with both actin microfilaments and microtubules (MTs). Structurally, MACF can be divided into 3 domains: an N-terminal domain that contains both a calponin type actin-binding domain and a plakin domain; a rod domain that is co...

journal_title：Journal of cell science

authors： Sun D,Leung CL,Liem RK

更新日期：2001-01-01 00:00:00

abstract：:Epithelial cell polarity is essential for animal development. The scaffold protein Bazooka (Baz/PAR-3) forms apical polarity landmarks to organize epithelial cells. However, it is unclear how Baz is recruited to the plasma membrane and how this is coupled with downstream effects. Baz contains an oligomerization domain...

journal_title：Journal of cell science

authors： McKinley RF,Yu CG,Harris TJ

更新日期：2012-03-01 00:00:00

abstract：:A new aspect of research into the pathogenesis of cystic fibrosis (CF) is a genetics-based search for ;modifier genes' that may affect the severity of CF lung disease. Using an alternative, cell biological approach, we show that ITPK1 should be considered a modifier gene. ITPK1 synthesizes an intracellular signal, ino...

journal_title：Journal of cell science

authors： Yang L,Reece J,Gabriel SE,Shears SB

更新日期：2006-04-01 00:00:00