Abstract:
:The growth of two human diploid skin fibroblast cell lines, originally grown in medium supplemented with foetal bovine serum and later adapted to medium supplemented with newborn bovine, bovine calf or horse serum, has been studied. Prolonged generation times increased cell volumes and decreased plating efficiencies were observed in cultures grown in newborn bovine, bovine calf or horse serum. In general, the deleterious effects were most severe as a result of growth in bovine calf or horse serum. In the light of the present findings, we believe investigators should exert great caution in switching human fibroblast cultures from foetal bovine serum to alternative sera, even at times of scarcity and high prices.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Zamansky GB,Arundel C,Nagasawa H,Little JBsubject
Has Abstractpub_date
1983-05-01 00:00:00pages
289-97eissn
0021-9533issn
1477-9137journal_volume
61pub_type
杂志文章abstract::The spindle checkpoint detects errors in kinetochore attachment to microtubules and delays anaphase if attachment is improper. The checkpoint is activated by attachment-sensitive components including Mad2 and certain phosphorylated proteins detected by the 3F3/2 antibody. We have studied Mad2 and 3F3/2 immunofluoresce...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::Separation of clathrin-coated pits from the plasma membrane, a key event during endocytosis, is thought to be driven by dynamin and the actin cytoskeleton. However, the mechanism for the actin-mediated endocytosis remains elusive. RNA interference-mediated suppression of cortactin, an F-actin binding protein that prom...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01668
更新日期:2005-02-15 00:00:00
abstract::During their development, mammalian oocytes acquire the ability to resume meiosis. We demonstrate that the concentration of p34cdc2 increases during the acquisition of meiotic competence, as determined by immunoblotting, whereas the concentration of cyclin B1 decreases. Laser-scanning confocal microscopy corroborated ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract::HC11 is a normal mouse mammary epithelial cell line that requires certain growth factors, such as EGF or bFGF, to respond optimally to lactogenic hormones and produce the differentiation marker beta-casein. Growth in insulin (Ins) or PDGF does not produce cells competent to respond to lactogenic hormones. Here we show...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::During spermatogenesis, the first morphological indication of spermatogonia differentiation is incomplete cytokinesis, followed by the assembly of stable intercellular cytoplasmic communications. This distinctive feature of differentiating male germ cells has been highly conserved during evolution, suggesting that reg...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00163
更新日期:2002-12-15 00:00:00
abstract::Truncated Bid (tBid) releases cytochrome c from mitochondria by inducing Bak (and Bax) pore formation in the outer membrane. An important issue is whether a second tBid action, independent of Bak and Bax, is also required to enhance cytochrome c mobility in the intermembrane spaces. To investigate this, we developed a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.021303
更新日期:2008-03-01 00:00:00
abstract::The mechanism responsible for spermatid translocation in the mammalian seminiferous epithelium was proposed to be the microtubule-based transport of specialized junction plaques (ectoplasmic specializations) that occur in Sertoli cell regions attached to spermatid heads. These plaques each consist of a cistern of endo...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::Nuclear sap, on fixation in glutaraldehyde, forms a fibrous network that resembles chromatin in its dimensions and staining properties. This artifactual network is easily confused with true chromatin fibres in sections of nuclei. With formaldehyde a homogeneous array of beads is produced. These are approximately 10 nm...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1977-08-01 00:00:00
abstract::The interaction of the non-receptor tyrosine kinase, Src, with the cytoskeleton of adhesion sites was studied in nerve growth cones isolated from fetal rat brain. Of particular interest was the role of protein tyrosine phosphatases in the regulation of Src-cytoskeleton binding. Growth cones were found to contain a hig...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract::Assembly of contractile apparatuses in striated muscle requires precisely regulated reorganization of the actin cytoskeletal proteins into sarcomeric organization. Regulation of actin filament dynamics is one of the essential processes of myofibril assembly, but the mechanism of actin regulation in striated muscle is ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.104950
更新日期:2012-09-01 00:00:00
abstract::The endoplasmic reticulum-Golgi intermediate compartment (ERGIC) defined by the cycling lectin ERGIC-53 consists of tubulovesicular clusters, but it is unknown if these membranes are transport vehicles or stationary entities. Here, we show by live imaging that GFP-ERGIC-53 mainly localizes to long-lived stationary and...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01615
更新日期:2005-01-15 00:00:00
abstract::Chloride intracellular channel 3 (CLIC3) drives invasiveness of pancreatic and ovarian cancer by acting in concert with Rab25 to regulate the recycling of α5β1 integrin from late endosomes to the plasma membrane. Here, we show that in two estrogen receptor (ER)-negative breast cancer cell lines, CLIC3 has little influ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.135947
更新日期:2014-09-15 00:00:00
abstract::Protein traffic moving from the endoplasmic reticulum (ER) to the Golgi complex in mammalian cells passes through the tubulovesicular membrane clusters of the ER-Golgi intermediate compartment (ERGIC), the marker of which is the lectin ERGIC-53. The dynamic nature and functional role of the ERGIC have been debated for...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.03019
更新日期:2006-06-01 00:00:00
abstract::Freeze-fracture of fixed and unfixed tissue, lanthanum tracer and conventional thin-section studies have revealed 2 new types of septate junction in the class Anthozoa, phylum Coelenterata. These new junctions have the 15-18-nm intercellular spacing of all other described septate junctions and are found around the api...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
abstract::ADP-ribosylation factors (Arfs) play central roles in the regulation of vesicular trafficking through the Golgi. Arfs are activated at the Golgi membrane by guanine-nucleotide-exchange factors (GEFs) that are recruited from cytosol. Here, we describe a novel mechanism for the regulation of recruitment and activity of ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.130591
更新日期:2014-01-15 00:00:00
abstract::Members of the ezrin-radixin-moesin (ERM) family of proteins are involved in multiple aspects of cell migration by acting both as crosslinkers between the membrane, receptors and the actin cytoskeleton, and as regulators of signalling molecules that are implicated in cell adhesion, cell polarity and migration. Increas...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.133108
更新日期:2014-01-15 00:00:00
abstract::Centaurin-alpha(1) is a phosphatidylinositol 3,4,5-trisphosphate binding protein as well as a GTPase activating protein (GAP) for the ADP-ribosylation factor (ARF) family of small GTPases. To further understand its cellular function, we screened a rat brain cDNA library using centaurin-alpha(1) as bait to identify cen...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02369
更新日期:2005-06-01 00:00:00
abstract::Regulation of the localization of mRNAs and local translation are universal features in eukaryotes and contribute to cellular asymmetry and differentiation. In Saccharomyces cerevisiae, localization of mRNAs that encode membrane proteins requires the She protein machinery, including the RNA-binding protein She2p, as w...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.149799
更新日期:2014-08-01 00:00:00
abstract::We uncovered a crucial role for the Aurora kinase A (AURKA)-Twist1 axis in promoting epithelial-to-mesenchymal transition (EMT) and chemoresistance in pancreatic cancer. Twist1 is the first EMT-specific target of AURKA that was identified using an innovative screen. AURKA phosphorylates Twist1 at three sites, which re...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.196790
更新日期:2017-03-15 00:00:00
abstract::Accurate chromosome segregation requires the spindle assembly checkpoint to be active at the onset of mitosis, before being silenced following chromosome alignment. p31(comet) is a checkpoint antagonist in that its inhibition delays mitotic exit, whereas its overexpression overrides the checkpoint. How exactly p31(com...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.093286
更新日期:2011-11-15 00:00:00
abstract::Mitogen-activated protein kinases (MAPKs) are conserved regulators of proliferation, differentiation and adaptation in eukaryotic cells. Their activity often involves changes in their subcellular localization, indicating an important role for these spatio-temporal dynamics in signal transmission. A striking model illu...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.213462
更新日期:2018-05-04 00:00:00
abstract::Retinoic acid (RA) plays key roles in cell differentiation and growth arrest by activating nuclear RA receptors (RARs) (α, β and γ), which are ligand-dependent transcription factors. RARs are also phosphorylated in response to RA. Here, we investigated the in vivo relevance of the phosphorylation of RARs during RA-ind...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.145979
更新日期:2014-05-01 00:00:00
abstract::Previous work has shown that phorbol esters or diacylglycerol trigger cortical granule exocytosis in Xenopus oocytes. We sought to identify the isoform(s) of protein kinase C (PKC) that mediate(s) this regulated secretory event. Because this process is initiated by lipid activators of PKC but is independent of calcium...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-03-15 00:00:00
abstract::TRF1 is a key component of the telomere-capping complex and binds double-strand telomeric DNA as homodimers. So far, it is not clear whether TRF1 dimerization coincides with its telomere binding or is actively controlled before it binds the telomere, and in the latter case, how this event might affect its telomere ass...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.089672
更新日期:2011-11-01 00:00:00
abstract::Cytoplasmic linker proteins (CLIPs) bind to microtubules and are proposed to link this cytoskeletal network to other intracellular structures. We are interested in CLIP-115, since this protein is enriched in neuronal dendrites and may operate in the control of brain-specific organelle translocations. Each CLIP monomer...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::The ryanodine receptor (RyR) is an ion channel composed of four identical subunits mediating calcium efflux from the endo/sarcoplasmic reticulum of excitable and non-excitable cells. We present several lines of evidence indicating that the RyR2 N-terminus is capable of self-association. A combination of yeast two-hybr...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.133538
更新日期:2013-11-01 00:00:00
abstract::Actomyosin stress fibers (SFs) enable cells to exert traction on planar extracellular matrices (ECMs) by tensing focal adhesions (FAs) at the cell-ECM interface. Although it is widely appreciated that the spatial and temporal distribution of these tensile forces play key roles in polarity, motility, fate choice, and o...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.119032
更新日期:2013-07-15 00:00:00
abstract::5'AMP nucleotidase activity was localized by electron microscopy in Dictyostelium discoideum during cell differentiation. In addition, the activity was assayed by micro enzymic methods in sections dissected from specific cellular regions of lyophilized individuals. The results of the 2 procedures were in agreement, de...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-10-01 00:00:00
abstract::Phosphoinositides regulate numerous processes in various subcellular compartments. Whereas many stimuli trigger changes in the plasma-membrane PtdIns(4,5)P(2) concentration, little is known about its precursor, PtdIns(4)P, in particular whether there are stimulus-induced alterations independent of those of PtdIns(4,5)...
journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
abstract::In human red blood cells, protein 4.1 (4.1R) stabilizes the spectrin-actin network and anchors it to the plasma membrane. To contribute to the characterization of functional roles of 4.1R in nonerythroid cells, we analysed the effect of ectopic expression of 4.1R isoforms on interphase microtubules in fibroblastic cel...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01544
更新日期:2004-12-01 00:00:00