Abstract：
:Truncated Bid (tBid) releases cytochrome c from mitochondria by inducing Bak (and Bax) pore formation in the outer membrane. An important issue is whether a second tBid action, independent of Bak and Bax, is also required to enhance cytochrome c mobility in the intermembrane spaces. To investigate this, we developed a kinetic analysis enabling changes in the diffusibility of cytochrome c in the intermembrane spaces of isolated mitochondria to be differentiated from changes resulting from Bak activation. Cytochrome c diffusibility in the intermembrane spaces was unaffected by changes in [tBid] over the range 0.5-19.0 pmol per mg of mitochondrial protein, when tBid-dependent Bak activation was increased several-thousand fold. However, high [tBid] (100 pmol mg(-1)) did increase diffusibility by approximately twofold. This was attributable to the permeability transition. Basal cytochrome c diffusibility in the intermembrane spaces in the absence of tBid was determined to be approximately 0.2 minute(-1), which is sufficient to support cytochrome c release with a half-time of 3.4 minutes. It is concluded that tBid has a monofunctional action at low concentrations and, more generally, that the basal cytochrome c diffusibility in the intermembrane spaces is adequate for rapid and complete cytochrome c release irrespective of the mode of outer membrane permeabilisation.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Gillick K,Crompton Mdoi
10.1242/jcs.021303subject
Has Abstractpub_date
2008-03-01 00:00:00pages
618-26issue
Pt 5eissn
0021-9533issn
1477-9137pii
jcs.021303journal_volume
121pub_type
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journal_title：Journal of cell science
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pub_type： 杂志文章
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journal_title：Journal of cell science
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doi：10.1242/jcs.173021
更新日期：2015-10-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2013-03-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1997-01-01 00:00:00
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journal_title：Journal of cell science
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