Abstract：
:Traction forces increase after microtubule depolymerization; however, the signaling mechanisms underlying this, in particular the dependence upon myosin II, remain unclear. We investigated the mechanism of traction force increase after nocodazole-induced microtubule depolymerization by applying traction force microscopy to cells cultured on micropatterned polyacrylamide hydrogels to obtain samples of homogeneous shape and size. Control cells and cells treated with a focal adhesion kinase (FAK) inhibitor showed similar increases in traction forces, indicating that the response is independent of FAK. Surprisingly, pharmacological inhibition of myosin II did not prevent the increase of residual traction forces upon nocodazole treatment. This increase was abolished upon pharmacological inhibition of FAK. These results suggest two distinct pathways for the regulation of traction forces. First, microtubule depolymerization activates a myosin-II-dependent mechanism through a FAK-independent pathway. Second, microtubule depolymerization also enhances traction forces through a myosin-II-independent, FAK-regulated pathway. Traction forces are therefore regulated by a complex network of complementary signals and force-generating mechanisms.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Rape A,Guo WH,Wang YLdoi
10.1242/jcs.090563subject
Has Abstractpub_date
2011-12-15 00:00:00pages
4233-40issue
Pt 24eissn
0021-9533issn
1477-9137pii
jcs.090563journal_volume
124pub_type
杂志文章abstract：:During gestation the epidermis develops from a single layer of ectoderm into a layer of keratinocytes overlaid by a layer of periderm; this is followed by a progressive increase in the number of layers of keratinocytes, until finally the distinct granular and cornified layers characteristic of mature epidermis are for...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:Excitatory amino acid transporter type 3 (EAAT3, also known as SLC1A1) is a high-affinity, Na(+)-dependent glutamate carrier that localizes primarily within the cell and at the apical plasma membrane. Although previous studies have reported proteins and sequence regions involved in EAAT3 trafficking, the detailed mole...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.185496
更新日期：2016-08-15 00:00:00
abstract：:The intracellular protozoan parasite Toxoplasma gondii, like all members of the phylum Apicomplexa, is known to possess many organelles: in addition to mitochondria and the compartments of the secretory pathway, there is a reduced chloroplast (the apicoplast) and the phylum-specific components of the apical complex: d...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：:In hepatocytes, PLIN2 is the major protein coating lipid droplets (LDs), an organelle the hepatitis C virus (HCV) hijacks for virion morphogenesis. We investigated the consequences of PLIN2 deficiency on LDs and on HCV infection. Knockdown of PLIN2 did not affect LD homeostasis, likely due to compensation by PLIN3, bu...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.217042
更新日期：2019-01-09 00:00:00
abstract：:Serial sections of mitotic spindles of the marine cryptophycean alga, Cryptomonas, were analysed to determine what types of microtubules they contained and which of these microtubules came close enough to each other (50 nm or less) for the commonly described crossbridging to be possible. Interpolar microtubules were r...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-06-01 00:00:00
abstract：:How vesicle trafficking components actively contribute to regulation of paracrine signaling is unclear. We genetically uncovered a requirement for α-soluble NSF attachment protein (α-Snap) in the activation of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway during Drosophila egg de...
journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2018-11-30 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：
更新日期：2002-01-01 00:00:00
abstract：:The progression of cells from metaphase to anaphase is thought to be regulated by a checkpoint that delays entry into anaphase until all chromosomes reach a stable bi-polar attachment at the metaphase plate. Previous work has suggested that the 3F3/2 kinetochore phosphoepitopes are involved in this checkpoint system. ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:Laminins are heterotrimeric glycoproteins of the extracellular matrix that are secreted by epithelial cells and which are crucial for the normal structure and function of the basement membrane. We have generated a mouse harboring a conditional knockout of α3 laminin (Lama3(fl/fl)), one of the main laminin subunits in ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.080911
更新日期：2011-09-01 00:00:00
abstract：:The cytoplasmic pH of growing pollen tubes of Lilium longiflorum Thunb. was measured using the pH-sensitive fluorescent dye 2',7'-bis-(carboxyethyl)-5(6')-carboxyfl uorescein and confocal fluorescence ratio imaging. The average cytoplasmic pH in the clear zone of the pollen tube tip was pH 7.11, and no consistent pH g...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
abstract：:Schistosomula of Schistosoma mansoni bind human low density lipoproteins (LDL) in a concentration-dependent and saturable manner. The bound LDL could provide phospholipids and sterol to the worm, which cannot synthesize sterol de novo and lacks acyl chain-modifying capability. Here we have used three phospholipid anal...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.205492
更新日期：2017-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:Truncated Bid (tBid) releases cytochrome c from mitochondria by inducing Bak (and Bax) pore formation in the outer membrane. An important issue is whether a second tBid action, independent of Bak and Bax, is also required to enhance cytochrome c mobility in the intermembrane spaces. To investigate this, we developed a...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.021303
更新日期：2008-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01158
更新日期：2004-07-01 00:00:00
abstract：:The HB2 cell line, developed from luminal epithelial cells cultured from milk, forms ball-like structures in collagen gels which show a uniform branching response to hepatocyte growth factor. The alpha2beta1 integrin is the major integrin expressed by luminal epithelial cells, and the role of this integrin in mammary ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：:Tenascin, an extracellular matrix protein that modulates cell adhesion, exists as a unique six-armed structure called a hexabrachion. The human hexabrachion is composed of six identical 320 kDa subunits and the structure is stabilized by inter-subunit disulfide bonds between amino-terminal segments. We have examined t...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:Although the 11.5 kDa Zn(2+)-binding protein (ZnBP, parathymosin-alpha) possesses a functional bipartite nuclear localization signal it was found in most tissues in the cytoplasm. The cultivation of freshly isolated rat hepatocytes for 24 hours under standard conditions was associated with an almost complete transloca...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
abstract：:In previous work we have shown that ovarian hormones, when injected into ovariectomized rats, alter the fine structure of the plasma membrane of endometrial epithelial cells. In this paper freeze-fractures have been used to study the apical plasma membrane of endometrial epithelial cells of rats during the period of b...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1982-06-01 00:00:00
abstract：:The Trypanosoma brucei flagellum is unusual as it is attached along the cell body and contains, in addition to an apparently conventional axoneme, a structure called the paraflagellar rod, which is essential for cell motility. Here, we investigated flagellum behaviour in normal and mutant trypanosome cell lines where ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03078
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.223883
更新日期：2019-02-01 00:00:00
abstract：:The small GTP-binding protein Rho rapidly stimulates the formation of focal adhesions and actin stress fibres when microinjected into serum-starved Swiss 3T3 fibroblasts. This response is inhibited by tyrosine kinase inhibitors. Addition of growth factors such as lysophosphatidic acid and bombesin to Swiss 3T3 cells s...
journal_title：Journal of cell science
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doi：
更新日期：1996-05-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2008-01-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1979-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2002-09-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:The structure of a late endosomal compartment, which contains the bulk of the cation-independent mannose 6-phosphate receptor (MPR) in NRK cells, is documented using immunocytochemistry and cryo-sections, as well as conventional Epon sections. This compartment, which we refer to as the prelysosomal compartment (PLC), ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
abstract：:Mitotic spindle assembly in animal cells is orchestrated by a chromosome-dependent pathway that directs microtubule stabilization. RanGTP generated at chromosomes releases spindle assembly factors from inhibitory complexes with importins, the nuclear transport factors that facilitate protein import into the nucleus du...
journal_title：Journal of cell science
pub_type： 杂志文章
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