ICAP-1 monoubiquitylation coordinates matrix density and rigidity sensing for cell migration through ROCK2-MRCKα balance.

abstract：:The regulation and function of the crucial cell cycle regulator cyclin E (CycE) remains elusive. Unlike other cyclins, CycE can be uniquely controlled by mitochondrial energetics, the exact mechanism being unclear. Using mammalian cells (in vitro) and Drosophila (in vivo) model systems in parallel, we show that CycE c...

journal_title：Journal of cell science

authors： Parker DJ,Iyer A,Shah S,Moran A,Hjelmeland AB,Basu MK,Liu R,Mitra K

更新日期：2015-11-15 00:00:00

abstract：:Ryanodine receptor subtype 1 (RyR1) has been primarily characterized in skeletal muscle but several studies have revealed its expression in smooth muscle. Here, we used Ryr1-null mice to investigate the role of this isoform in Ca(2+) signaling in urinary bladder smooth muscle. We show that RyR1 is required for depolar...

journal_title：Journal of cell science

authors： Fritz N,Morel JL,Jeyakumar LH,Fleischer S,Allen PD,Mironneau J,Macrez N

更新日期：2007-11-01 00:00:00

abstract：:The surface coat of procyclic forms of Trypanosoma brucei consists of related, internally repetitive glycoproteins known as EP and GPEET procyclins. Previously we showed that the extracellular domain of GPEET is phosphorylated. We now show that phosphorylation of this glycosylphosphatidylinositol-anchored surface prot...

journal_title：Journal of cell science

authors： Bütikofer P,Vassella E,Ruepp S,Boschung M,Civenni G,Seebeck T,Hemphill A,Mookherjee N,Pearson TW,Roditi I

更新日期：1999-06-01 00:00:00

abstract：:When green cells of Euglena gracilis, strain Z, were light-grown for several months on a solid medium containing an excess of sodium acetate (1.0% instead of the normal 0.1%), some 30% of the cells were colourless. The 'acetate-bleached' organisms, isolated by plating methods and subsequently incubated in the light in...

journal_title：Journal of cell science

authors： Vannini GL

更新日期：1983-05-01 00:00:00

abstract：:The cell nucleus is highly organized. Many nuclear functions are localized in discrete domains, suggesting that compartmentalization is an important aspect of the regulation and coordination of nuclear functions. We investigated the subnuclear distribution of the glucocorticoid receptor, a hormone-dependent transcript...

journal_title：Journal of cell science

authors： van Steensel B,Brink M,van der Meulen K,van Binnendijk EP,Wansink DG,de Jong L,de Kloet ER,van Driel R

更新日期：1995-09-01 00:00:00

abstract：:Occludin plays an important role in maintaining tight junction barrier function in many types of epithelia. We previously reported that activation of transient receptor potential vanilloid subtype 1 (TRPV1) in rabbit submandibular gland promoted salivary secretion, partly by an increase in paracellular permeability. W...

journal_title：Journal of cell science

authors： Cong X,Zhang Y,Yang NY,Li J,Ding C,Ding QW,Su YC,Mei M,Guo XH,Wu LL,Yu GY

更新日期：2013-03-01 00:00:00

abstract：:Tie2-promoter-mediated loss of peroxisome proliferator-activated receptor gamma (PPARγ, also known as PPARG) in mice leads to osteopetrosis and pulmonary arterial hypertension. Vascular disease is associated with loss of PPARγ in pulmonary microvascular endothelial cells (PMVEC); we evaluated the role of PPARγ in PMVE...

journal_title：Journal of cell science

authors： Vattulainen-Collanus S,Akinrinade O,Li M,Koskenvuo M,Li CG,Rao SP,de Jesus Perez V,Yuan K,Sawada H,Koskenvuo JW,Alvira C,Rabinovitch M,Alastalo TP

更新日期：2016-02-15 00:00:00

abstract：:Tau is a neuronal microtubule-associated protein that appears to function in the formation and maintenance of axons by influencing microtubule organisation. Tau is a phosphoprotein and is more heavily phosphorylated in fetal than in adult brain, and is also hyperphosphorylated in Alzheimer's disease where it forms the...

journal_title：Journal of cell science

authors： Wagner U,Utton M,Gallo JM,Miller CC

更新日期：1996-06-01 00:00:00

abstract：:The human adenocarcinoma cell line Caco-2 was used for studies on the biosynthesis and transport of lysosomal acid alpha-glucosidase in polarized epithelial cells. Metabolic labelling revealed that in Caco-2 cells alpha-glucosidase is synthesized as a precursor form of 110 x 10(3) Mr. This form is converted into a pre...

journal_title：Journal of cell science

authors： Klumperman J,Fransen JA,Boekestijn TC,Oude Elferink RP,Matter K,Hauri HP,Tager JM,Ginsel LA

更新日期：1991-10-01 00:00:00

abstract：:Autophagy requires the formation of membrane vesicles, known as autophagosomes, that engulf cellular cargoes and subsequently recruit lysosomal hydrolases for the degradation of their contents. A number of autophagy-related proteins act to mediate the de novo biogenesis of autophagosomes and vesicular trafficking even...

journal_title：Journal of cell science

authors： Gammoh N

更新日期：2020-10-30 00:00:00

abstract：:Trypsin digestion of demembranated fowl spermatozoa caused a longitudinal splitting of the distal part of the axoneme. The resulting strands, consisting of groups of doublet microtubules, formed left-handed helices. On the evidence of electron micrographs, the digestion had caused the loss of dynein arms from the oute...

journal_title：Journal of cell science

authors： Woolley DM,Brammall A

更新日期：1987-10-01 00:00:00

abstract：:Myogenic differentiation in adult muscle is normally suppressed and can be activated by myogenic cues in a subset of activated satellite cells. The switch mechanism that turns myogenesis on and off is not defined. In the present study, we demonstrate that tissue inhibitor of metalloproteinase 3 (TIMP3), the endogenous...

journal_title：Journal of cell science

authors： Liu H,Chen SE,Jin B,Carson JA,Niu A,Durham W,Lai JY,Li YP

更新日期：2010-09-01 00:00:00

abstract：:U937 human promonocytic leukemia cells express PKC isozymes beta 1, beta 2, epsilon and zeta. Indirect immunocytofluorescence using affinity-purified PKC-specific antibodies indicates that each of the endogenous PKC isozymes in U937 cells display a unique compartmentalization within the intact cell. PKC-beta 1 is dist...

journal_title：Journal of cell science

authors： Kiley SC,Parker PJ

更新日期：1995-03-01 00:00:00

abstract：:Ligand-dependent nuclear import is crucial for the function of the androgen receptor (AR) in both health and disease. The unliganded AR is retained in the cytoplasm but, on binding 5alpha-dihydrotestosterone, it translocates into the nucleus and alters transcription of its target genes. Nuclear import of AR is mediate...

journal_title：Journal of cell science

authors： Cutress ML,Whitaker HC,Mills IG,Stewart M,Neal DE

更新日期：2008-04-01 00:00:00

abstract：:DNA replication in eukaryotic cells initiates from multiple replication origins that are distributed throughout the genome. Coordinating the usage of these origins is crucial to ensure complete and timely replication of the entire genome precisely once in each cell cycle. Replication origins fire according to a cell-t...

journal_title：Journal of cell science

authors： Yekezare M,Gómez-González B,Diffley JF

更新日期：2013-03-15 00:00:00

abstract：:Onconase is an RNase with a very specific property because it is selectively toxic to transformed cells. This toxin is thought to recognize cell surface receptors, and the protection conferred by metabolic poisons against Onconase toxicity indicated that this RNase relies on endocytic uptake to kill cells. Nevertheles...

journal_title：Journal of cell science

authors： Rodríguez M,Torrent G,Bosch M,Rayne F,Dubremetz JF,Ribó M,Benito A,Vilanova M,Beaumelle B

更新日期：2007-04-15 00:00:00

abstract：:It has long been established that neuronal growth cone navigation depends on changes in microtubule (MT) and F-actin architecture downstream of guidance cues. However, the mechanisms by which MTs and F-actin are dually coordinated remain a fundamentally unresolved question. Here, we report that the well-characterized ...

journal_title：Journal of cell science

authors： Slater PG,Cammarata GM,Samuelson AG,Magee A,Hu Y,Lowery LA

更新日期：2019-04-30 00:00:00

abstract：:Ubiquitinated membrane proteins such as epidermal growth factor receptor (EGFR) are delivered to early endosomes and then sorted to lysosomes via multivesicular bodies (MVBs) for degradation. The regulatory mechanism underlying formation of intralumenal vesicles en route to generation of MVBs is not fully understood. ...

journal_title：Journal of cell science

authors： Hasegawa J,Jebri I,Yamamoto H,Tsujita K,Tokuda E,Shibata H,Maki M,Itoh T

更新日期：2019-10-03 00:00:00

abstract：:The surface of the ciliate Dileptus contains locomotor and sensory domains. Each domain contains cilia of its own kind, arranged in a characteristic pattern. Transformation of a portion of the locomotor area into the sensory domain has been studied. Locomotor ciliary units in the transforming area undergo resorption o...

journal_title：Journal of cell science

authors： Golinska K

更新日期：1983-07-01 00:00:00

abstract：:The conversion of the cellular prion protein (PrPc) into a pathogenic isoform (PrP(Sc)) is one of the underlying events in the pathogenesis of the fatal transmissible spongiform encephalopathies (TSEs). Numerous compounds have been described to inhibit prion replication and PrP(Sc) accumulation in cell culture. Among ...

journal_title：Journal of cell science

authors： Nunziante M,Kehler C,Maas E,Kassack MU,Groschup M,Schätzl HM

更新日期：2005-11-01 00:00:00

abstract：:The formation and maturation of membrane carriers that transport cargo from the ER to the Golgi complex involves the sequential action of the coat protein complexes COPII and COPI. Recruitment of COPI to nascent carriers requires activation of ADP-ribosylation factors by a BrefeldinA-sensitive guanine nucleotide excha...

journal_title：Journal of cell science

authors： Zhao X,Claude A,Chun J,Shields DJ,Presley JF,Melançon P

更新日期：2006-09-15 00:00:00

abstract：:The cellular microenvironment, characterized by an extracellular matrix (ECM), played an essential role in the transition from unicellularity to multicellularity in animals (metazoans), and in the subsequent evolution of diverse animal tissues and organs. A major ECM component are members of the collagen superfamily -...

journal_title：Journal of cell science

authors： Fidler AL,Boudko SP,Rokas A,Hudson BG

更新日期：2018-04-09 00:00:00

abstract：:Rab GTPases regulate various membrane trafficking pathways but the mechanisms by which GTPase-activating proteins recognise specific Rabs are not clear. Rab8 is involved in controlling several trafficking processes, including the trafficking of transferrin receptor from the early endosome to the recycling endosome. He...

journal_title：Journal of cell science

authors： Vaibhava V,Nagabhushana A,Chalasani ML,Sudhakar C,Kumari A,Swarup G

更新日期：2012-11-01 00:00:00

abstract：:Skeletal and heart muscle-specific variant of the α subunit of nascent polypeptide associated complex (skNAC; encoded by NACA) is exclusively found in striated muscle cells. Its function, however, is largely unknown. Previous reports have demonstrated that skNAC binds to m-Bop/Smyd1, a multi-functional protein that re...

journal_title：Journal of cell science

authors： Berkholz J,Michalick L,Munz B

更新日期：2014-09-01 00:00:00

abstract：:Most tissues harbor a substantial population of resident macrophages. Here, we elucidate a functional link between the Slc7a7 cationic amino acid transporter and tissue macrophages. We identified a mutant zebrafish devoid of microglia due to a mutation in the slc7a7 gene. We found that in Slc7a7-deficient larvae, macr...

journal_title：Journal of cell science

authors： Demy DL,Carrère M,Noche R,Tauzin M,Le Bris M,Baek C,Leshchiner I,Goessling W,Herbomel P

更新日期：2020-10-22 00:00:00

abstract：:The mucolipin (TRPML) subfamily of transient receptor potential (TRP) cation channels consists of three members that play various roles in the regulation of membrane and protein sorting along endo-lysosomal pathways. Loss-of-function mutations in TRPML1 cause the neurodegenerative lysosomal storage disorder, mucolipid...

journal_title：Journal of cell science

authors： Zeevi DA,Lev S,Frumkin A,Minke B,Bach G

更新日期：2010-09-15 00:00:00

abstract：:The constitutive recirculation of lymphocytes between the widely distributed organs of the immune system is essential for host defence. We have developed an in vitro model of lymphocyte migration from the blood into lymph nodes which employs primary cultures of high endothelial cells (HEC). HEC-adherent lymphocytes ad...

journal_title：Journal of cell science

authors： Hourihan H,Allen TD,Ager A

更新日期：1993-04-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) replication and transcription are crucial for cell function, but these processes are poorly understood at the single-cell level. We describe a novel fluorescence in situ hybridization protocol, called mTRIP (mitochondrial transcription and replication imaging protocol), that reveals simultane...

journal_title：Journal of cell science

authors： Chatre L,Ricchetti M

更新日期：2013-02-15 00:00:00

abstract：:We use the fungus Ashbya gossypii to investigate how its polar growth machinery is organized to achieve sustained hyphal growth. In slowly elongating hyphae exocyst, cell polarity and polarisome proteins permanently localize as cortical cap at hyphal tips, thus defining the zone of secretory vesicle fusion. In tenfold...

journal_title：Journal of cell science

authors： Köhli M,Galati V,Boudier K,Roberson RW,Philippsen P

更新日期：2008-12-01 00:00:00

abstract：:Serial sections of mitotic spindles of the marine cryptophycean alga, Cryptomonas, were analysed to determine what types of microtubules they contained and which of these microtubules came close enough to each other (50 nm or less) for the commonly described crossbridging to be possible. Interpolar microtubules were r...

journal_title：Journal of cell science

authors： Oakley BR,Heath IB

更新日期：1978-06-01 00:00:00