Abstract:
:When green cells of Euglena gracilis, strain Z, were light-grown for several months on a solid medium containing an excess of sodium acetate (1.0% instead of the normal 0.1%), some 30% of the cells were colourless. The 'acetate-bleached' organisms, isolated by plating methods and subsequently incubated in the light in a liquid medium, regained the capacity to form chlorophyll in a few days in the absence of any organic carbon source, and within 1-2 weeks in the presence of 0.1% acetate. A number of bleached colonies, however, gave rise to populations in which the delay in pigment synthesis initiation was at least 2 months. Besides numerous paramylum granules and lipid inclusions, the acetate-bleached cells exhibited variably shaped and sized plastids, apparently lacking in ribosomes and showing a deeply disorganized membrane system. In the alga greened in the presence of 0.1% acetate, the pattern of plastidome reorganization was altered; the thylakoids were often unpaired and vesiculated in different degrees, owing primarily to the swelling of the lumen. A complete recovery of normal chloroplast structure occurred only after several weeks of exponential growth. The entire population greened in the absence of acetate constantly showed normal chloroplasts with perfectly reassociated thylakoids and clear partitions.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Vannini GLsubject
Has Abstractpub_date
1983-05-01 00:00:00pages
413-22eissn
0021-9533issn
1477-9137journal_volume
61pub_type
杂志文章abstract::This study focuses on the effect of static and dynamic mechanical compression on the biosynthetic activity of chondrocytes cultured within agarose gel. Chondrocyte/agarose disks (3 mm diameter) were placed between impermeable platens and subjected to uniaxial unconfined compression at various times in culture (2-43 da...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::p97 (CDC-48 in Caenorhabditis elegans) is a ubiquitin-selective AAA (ATPases associated with diverse cellular activities) chaperone and its key function is to disassemble protein complexes. p97 functions in diverse cellular processes including endoplasmic reticulum (ER)-associated degradation, membrane fusion, and mei...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.052415
更新日期:2009-10-15 00:00:00
abstract::T cell activation is accompanied by actin-mediated receptor clustering and reorganization of lipid rafts. It has been suggested that costimulatory molecules might be involved in these processes. We here provide evidence that engagement of the adhesion molecule CD44 initiates cytoskeletal rearrangement and membrane reo...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::We have previously shown by examining the anchorage dependence, density-dependent inhibition of growth, LETS protein and microfilament bundles that the transformed phenotype of the parental tumours are suppressed in hybrids between rat embryo fibroblast (REF) and mouse tumour cells (TA3B). Hybrids between TA3B and Syr...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-10-01 00:00:00
abstract::A comparison of stained cytosolic proteins, in vivo 80 S ribosome translation products and in vitro translation products of poly(A)+ RNA from three species of Acetabularia was performed after characterization of their molecular weights and isoelectric points via two-dimensional electrophoresis. A total of 803 stained ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1983-03-01 00:00:00
abstract::We have reported earlier that the polyphosphoinositide messenger system may control mitosis in sea urchin eggs. Besides phospholipase C activation and its second messengers, phosphatidylinositol (PI) 3-kinase has been proposed to affect a wide variety of cellular processes in other cellular systems. Therefore, we have...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::To understand the mechanism involved in the apical sorting of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) we fused to the C-terminus of GFP the GPI-anchor-attachment signal of the folate receptor (FR) or of the prion protein (PrP), two native GPI-anchored proteins that are sorted apically or basolat...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.036038
更新日期:2008-12-15 00:00:00
abstract::A polyclonal antibody was raised to the C-terminal region of fission yeast BiP. The use of this antibody for immunoprecipitation, western blotting and immunofluorescence has confirmed and extended the observations made previously with an epitope-tagged BiP molecule. A fraction of BiP protein is glycosylated in Schizos...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
abstract::Filamin (actin-binding protein) is a cytoskeletal protein that crosslinks actin filaments in vitro. Filamin is thought to be involved in a variety of cell types in stabilizing actin networks, and in platelets it may play a role in linking actin to the membrane. In this report, we describe a monoclonal antibody (Mab 6E...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1989-09-01 00:00:00
abstract::The SPN antigen plays an essential role in mitosis, since microinjection of antibodies causes mitotic arrest. Here we show, by examination of the relative locations of SPN antigen, the centrosomal 5051 antigen and tubulin in normal mitotic, and in taxol-treated mitotic cells, that the SPN antigen is involved in organi...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract::New crystal structures of the kinesin motors differ from previously described motor-ADP atomic models, showing striking changes both in the switch I region near the nucleotide-binding cleft and in the switch II or 'relay' helix at the filament-binding face of the motor. The switch I region, present as a short helix fl...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:
更新日期:2002-01-01 00:00:00
abstract::Mucin expression was analysed, in relation to cell growth, in parental HT-29 cells and in two populations of mucus-secreting HT-29 cells selected by adaptation to methotrexate (HT29-MTX) or 5-fluorouracil (HT29-FU). These two populations express mature mucins that differ in their immunoreactivity to antibodies against...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract::Major histocompatibility complex class I (MHC-I) molecules signal infection or transformation by engaging receptors on T lymphocytes. The spatial organization of MHC-I on the plasma membranes is important for this engagement. We and others have shown that MHC-I molecules, like other membrane proteins, are not uniforml...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.187112
更新日期:2016-09-01 00:00:00
abstract::The fractionation of human liver mitochondria into inner membrane, outer membrane and matrix material is reported. Compared with rat, human liver mitochondria are more fragile. Fractionation can be achieved in only 2 steps, a digitonin treatment for removal of the outer membrane and centrifugation of the inner membran...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-02-01 00:00:00
abstract::The centrosome and cilium are organelles with important roles in microtubule organisation, cell division, cell signalling, embryogenesis and tissue homeostasis. The two organelles are mutually exclusive. The centriole/basal body is found at the core of the centrosome (centriole) or at the base of the cilium (basal bod...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.196642
更新日期:2017-10-01 00:00:00
abstract::By passing a suspension of polymorphonuclear leucocytes through a parallel-plate chamber their adhesiveness can be assessed by scoring the number of cells trapped on the lower plate, and the fluid shear stress can be defined for a given flow rate. Since the adhesiveness of the cell at the instant of collision must exc...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1984-08-01 00:00:00
abstract::Neutrophil extracellular traps (NETs) are one of the most intriguing discoveries in immunological research of the past few years. After their first description in 2004, the number of research articles on how NETs affect immunodefense, and also how they contribute to an ever-growing number of diseases, has skyrocketed....
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.241075
更新日期:2020-03-10 00:00:00
abstract::We report the first direct analysis of the endogenous β-catenin phosphorylation activity in colon cancer SW480 cells. By comparing parental SW480 cells that harbor a typical truncated adenomatous polyposis coli (APC) form, cells expressing full-length APC and APC-depleted cells, we provide the formal demonstration tha...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.131045
更新日期:2014-04-15 00:00:00
abstract::The tetrapeptide N-Acetyl-Seryl-Aspartyl-Lysyl-Proline (AcSDKP) is a natural regulator of hematopoietic stem cell proliferation. The present study was aimed at investigating the presence and the role of AcSDKP in rat testis. Specific immunoreactivity was always observed in the interstitial tissue at all stages of test...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::The nature, kinetics and cofactor requirements of leukocyte adhesion to cultured vascular cells have been investigated in vitro, using a model system in which leukocyte suspensions are in continuous motion over the cultured cells. Adhesion is assessed by histological examination or by using 51Cr-labelled leukocytes. L...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-10-01 00:00:00
abstract::Targeting of glycosylphosphatidylinositol-anchored proteins to the apical surface of epithelial cells involves clustering in Triton X-100-resistant membrane microdomains or rafts. The role of these microdomains in sorting transmembrane proteins is more questionable because, unlike glycosylphosphatidylinositol-anchored...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.002717
更新日期:2007-03-15 00:00:00
abstract::The ret finger protein (rfp) is a member of the B-box zinc finger gene family many of which may function in growth regulation and in the appropriate context become oncogenic. Members of this family are nuclear proteins that possess a characteristic tripartite motif consisting of the RING and B-box zinc binding domains...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
abstract::The interaction of the non-receptor tyrosine kinase, Src, with the cytoskeleton of adhesion sites was studied in nerve growth cones isolated from fetal rat brain. Of particular interest was the role of protein tyrosine phosphatases in the regulation of Src-cytoskeleton binding. Growth cones were found to contain a hig...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract::The activated leukocyte cell adhesion molecule (ALCAM) is dynamically regulated by the actin cytoskeleton. In this study we explored the molecular mechanisms and signaling pathways underlying the cytoskeletal restraints of this homotypic adhesion molecule. We observed that ALCAM-mediated adhesion induced by cytoskelet...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01139
更新日期:2004-06-01 00:00:00
abstract::The family of integrin transmembrane receptors is essential for the normal function of multicellular organisms by facilitating cell-extracellular matrix adhesion. The vitronectin-binding integrin αVβ5 localizes to focal adhesions (FAs) as well as poorly characterized flat clathrin lattices (FCLs). Here, we show that, ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.221317
更新日期:2018-11-05 00:00:00
abstract::Poly(ADP-ribose) polymerase-1 (PARP1) is a nuclear enzyme that can trigger caspase-independent necrosis. Two main mechanisms for this have been proposed: one involving RIP1 and JNK kinases and mitochondrial permeability transition (MPT), the other involving calpain-mediated activation of Bax and mitochondrial release ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.128009
更新日期:2014-10-01 00:00:00
abstract::Microinjected GTP gamma S revealed three distinct steps in the exocytic transport of the temperature sensitive glycoprotein of vesicular stomatitis virus (ts-O45-G) from the ER to the cell surface in intact Vero cells. While COPII dependent export of ts-O45-G from the ER is blocked in cells injected with recombinant p...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract::In neutrophils and other phagocytic cells, the small GTPase Rac is an essential regulator of a multi-component NADPH oxidase that produces high levels of superoxide, which kills invading pathogens. In many other cell types, Rac and newly discovered relatives of the neutrophil burst oxidase and its subunits have been f...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.00937
更新日期:2004-01-15 00:00:00
abstract::Long noncoding RNAs (lncRNAs) have emerged as important regulators of cancer progression. Abnormal sialylation leads to renal cell carcinoma (RCC) malignancy. However, the mechanism by which the lncRNA maternally expressed gene 3 (MEG3) mediates RCC progression by regulating ST3Gal1 transcription and EGFR sialylation ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.244020
更新日期:2020-08-25 00:00:00
abstract::Apical and basolateral endocytic pathways in polarised Caco-2 cells were investigated by following the uptake, recycling and transcytosis of the galactose-binding protein toxin ricin, as a membrane marker. Differences in the extent and kinetics of lectin uptake, recycling and transcytosis were observed at the apical a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00