Abstract：
:Most tissues harbor a substantial population of resident macrophages. Here, we elucidate a functional link between the Slc7a7 cationic amino acid transporter and tissue macrophages. We identified a mutant zebrafish devoid of microglia due to a mutation in the slc7a7 gene. We found that in Slc7a7-deficient larvae, macrophages do enter the retina and brain to become microglia, but then die during the developmental wave of neuronal apoptosis, which triggers intense efferocytic work from them. A similar macrophage demise occurs in other tissues, at stages where macrophages have to engulf many cell corpses, whether due to developmental or experimentally triggered cell death. We found that Slc7a7 is the main cationic amino acid transporter expressed in macrophages of zebrafish larvae, and that its expression is induced in tissue macrophages within 1-2 h upon efferocytosis. Our data indicate that Slc7a7 is vital not only for microglia but also for any steadily efferocytic tissue macrophages, and that slc7a7 gene induction is one of the adaptive responses that allow them to cope with the catabolism of numerous dead cells without compromising their own viability.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Demy DL,Carrère M,Noche R,Tauzin M,Le Bris M,Baek C,Leshchiner I,Goessling W,Herbomel Pdoi
10.1242/jcs.249037subject
Has Abstractpub_date
2020-10-22 00:00:00issue
20eissn
0021-9533issn
1477-9137pii
jcs.249037journal_volume
133pub_type
杂志文章abstract：:Delayed wound healing caused by inefficient re-epithelialization underlines chronic skin lesions such as those found in diabetes. The dioxin receptor (AhR) modulates cell plasticity and migration and its activation by occupational polycyclic aromatic hydrocarbons (PAHs) results in severe skin lesions such as contact h...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.047274
更新日期：2009-06-01 00:00:00
abstract：:C-CAM is a Ca(2+)-independent cell adhesion molecule (CAM) that mediates intercellular adhesion of isolated rat hepatocytes. It is widely distributed in epithelia, where its presence both at lateral cell borders and on apical cell surfaces suggests that it may have diverse biological functions. Two major isoforms, C-C...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:The function of DAXX, a highly conserved mammalian gene, has remained controversial; this is due, in part, to its identification in a variety of yeast two-hybrid screens. Targeted deletion in the mouse revealed that DAXX is essential for embryonic development. Furthermore, the increased levels of apoptosis observed in...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00234
更新日期：2003-01-15 00:00:00
abstract：:The cell nucleus is highly organized. Many nuclear functions are localized in discrete domains, suggesting that compartmentalization is an important aspect of the regulation and coordination of nuclear functions. We investigated the subnuclear distribution of the glucocorticoid receptor, a hormone-dependent transcript...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:We have used genetic and molecular techniques to investigate the interactions among genes required for the initiation and regulation of septum formation in Schizosaccharomyces pombe. Our data suggest that the products of the cdc7, cdc11, cdc14 and cdc16 genes interact. These activities may regulate the function of the...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：:The p53 tumor suppressor protein is a key regulator of cellular functions including responses to numerous stress signals, and triggers apoptosis in many cell types, including neurons. The major mechanisms known to regulate p53 stabilization and activation include phosphorylation and ubiquitin ligase-mediated proteasom...
journal_title：Journal of cell science
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doi：10.1242/jcs.03468
更新日期：2007-07-01 00:00:00
abstract：:Every two years, the French Society for Cell Biology (SBCF) organises an international meeting called 'Imaging the Cell'. This year, the 8th edition was held on 24-26 June 2015 at University of Bordeaux Campus Victoire in the city of Bordeaux, France, a UNESCO World Heritage site. Over the course of three days, the me...
journal_title：Journal of cell science
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doi：10.1242/jcs.180042
更新日期：2015-11-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1999-10-01 00:00:00
abstract：:Glomerular basement membrane (GBM) and podocalyxin are essential for podocyte morphology. We provide evidence of functional interconnections between basement membrane components (collagen IV and laminin), the expression of podocalyxin and the morphology of human glomerular epithelial cells (podocytes). We demonstrated...
journal_title：Journal of cell science
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doi：10.1242/jcs.01163
更新日期：2004-07-01 00:00:00
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更新日期：2005-12-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:Wnts are glycan- and lipid-modified morphogens that are important for cellular responses, but how Wnts are secreted in polarized epithelial cells remains unclear. Although Wntless (Wls) has been shown to interact with Wnts and support their secretion, the role of Wls in the sorting of Wnts to the final destination in ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.126052
更新日期：2013-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02588
更新日期：2005-10-15 00:00:00
abstract：:Wound healing crucially relies on the mechanical activity of fibroblasts responding to TGFβ1 and to forces transmitted across focal adhesions. Integrin-linked kinase (ILK) is a central adapter recruited to integrin β1 tails in focal adhesions mediating the communication between cells and extracellular matrix. Here, we...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.063024
更新日期：2010-11-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:The Wnt-5a gene encodes a secreted protein that controls several normal processes during embryogenesis and development of adult tissues by as yet unknown mechanisms. Endogenous expression of Wnt-5a mRNA is known to occur in both mouse and human mammary cell lines. To investigate the biological role of Wnt-5a in the hu...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-06-01 00:00:00
abstract：:We uncovered a crucial role for the Aurora kinase A (AURKA)-Twist1 axis in promoting epithelial-to-mesenchymal transition (EMT) and chemoresistance in pancreatic cancer. Twist1 is the first EMT-specific target of AURKA that was identified using an innovative screen. AURKA phosphorylates Twist1 at three sites, which re...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.196790
更新日期：2017-03-15 00:00:00
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journal_title：Journal of cell science
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doi：10.1242/jcs.222893
更新日期：2019-01-03 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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journal_title：Journal of cell science
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doi：
更新日期：1978-04-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1996-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-08-01 00:00:00
