Abstract:
:Differentially expressed membrane antigens have been used to flow-sort viable luminal epithelial and myoepithelial cells from freshly disaggregated adult virgin rat mammary parenchyma. Resulting cultures and clones have been characterized morphologically and by a panel of antibodies that recognise cell-type-specific cytoskeletal antigens in the intact mammary gland. Five clonal phenotypes were recognisable by morphological criteria, three (types 1-3) exclusively associated with sorted luminal epithelial (25.5-positive) cells, and two (types 4 and 5) generated from the sorted myoepithelial (CALLA/neutral endopeptidase 24.11-positive) cells. All clones derived from myoepithelial cells continued to express a basal parenchymal marker in the form of the rat equivalent of human cytokeratin 14, while smooth muscle alpha-actin was expressed by the small slowly growing type 4 clones but was found in fewer cells in rapidly proliferating type 5 myoepithelially derived clones. Two of the luminal clone types, characterized by an attenuated appearance and slow growth (types 1/2), expressed only luminal-specific markers, including the equivalent of human cytokeratins 7/18/19. Type 3 clones, by contrast, consisted of rapidly proliferating cells, many of which either co-expressed CK14 and CK18 antigens (type 3a) or were composed of a mosaic of CK14+/CK18-, CK14+/CK18+ and CK14-/CK18+ cells (type 3b). The sorted myoepithelially derived clones grew faster than clones from sorted luminal cells as evidenced by the larger fraction of cells synthesizing DNA. All types of clone could be obtained from both isolated ducts and alveoli, when these were cloned separately, although there were some differences in their relative frequency.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Dundas SR,Ormerod MG,Gusterson BA,O'Hare MJsubject
Has Abstractpub_date
1991-11-01 00:00:00pages
459-71eissn
0021-9533issn
1477-9137journal_volume
100 ( Pt 3)pub_type
杂志文章abstract::Plastids in plants and algae evolved from the endosymbiotic integration of a cyanobacterium by a heterotrophic eukaryote. New plastids can only emerge through fission; thus, the synchronization of bacterial division with the cell cycle of the eukaryotic host was vital to the origin of phototrophic eukaryotes. Most of ...
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1993-12-01 00:00:00
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journal_title:Journal of cell science
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2013-01-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2008-12-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2016-08-15 00:00:00
abstract::To enhance our understanding of the function(s) of gamma-tubulin-complex proteins (GCPs), we identified and analyzed the functions of the Aspergillus nidulans homologs of GCP2-GCP6 (here designated GCPB-GCBF). The gamma-tubulin small complex (gamma-TuSC) components, gamma-tubulin, GCPB and GCPC, are essential for viab...
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pub_type: 杂志文章
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更新日期:2009-11-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
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journal_title:Journal of cell science
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doi:
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1982-06-01 00:00:00
abstract::Prion diseases are fatal neurodegenerative diseases characterised by the accumulation of misfolded prion protein (PrP(Sc)) in the brain. They are caused by the templated misfolding of normal cellular protein, PrP(C), by PrP(Sc). We have recently generated a unique cell system in which epitope-tagged PrP(C) competent t...
journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2013-08-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
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