Abstract：
:The nimO predicted protein of Aspergillus nidulans is related structurally and functionally to Dbf4p, the regulatory subunit of Cdc7p kinase in budding yeast. nimOp and Dbf4p are most similar in their C-termini, which contain a PEST motif and a novel, short-looped Cys2-His2 zinc finger-like motif. DNA labelling and reciprocal shift assays using ts-lethal nimO18 mutants showed that nimO is required for initiation of DNA synthesis and for efficient progression through S phase. nimO18 mutants abrogated a cell cycle checkpoint linking S and M phases by segregating their unreplicated chromatin. This checkpoint defect did not interfere with other checkpoints monitoring spindle assembly and DNA damage (dimer lesions), but did prevent activation of a DNA replication checkpoint. The division of unreplicated chromatin was accelerated in cells lacking a component of the anaphase-promoting complex (bimEAPC1), consistent with the involvement of nimO and APC/C in separate checkpoint pathways. A nimO deletion conferred DNA synthesis and checkpoint defects similar to nimO18. Inducible nimO alleles lacking as many as 244 C-terminal amino acids supported hyphal growth, but not asexual development, when overexpressed in a ts-lethal nimO18 strain. However, the truncated alleles could not rescue a nimO deletion, indicating that the C terminus is essential and suggesting some type of interaction among nimO polypeptides.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
James SW,Bullock KA,Gygax SE,Kraynack BA,Matura RA,MacLeod JA,McNeal KK,Prasauckas KA,Scacheri PC,Shenefiel HL,Tobin HM,Wade SDkeywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
1313-24eissn
0021-9533issn
1477-9137journal_volume
112 ( Pt 9)pub_type
杂志文章abstract：:The atomic force microscope (AFM) was used to directly image hippocampal neurons and glia. Using chemically fixed and living cells it was possible to reconstruct three-dimensional cell structure and detect sub-cellular features such as the nucleus, mitochondria and filaments. By repeatedly scanning a single living cel...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:We have investigated the effect of nitric oxide (NO) on apoptosis in Swiss 3T3 fibroblasts and compared it to the effect of the nitrosonium cation (NO+). Both species induced apoptosis, confirmed by electron microscopy, propidium iodide staining, DNA laddering and activation of caspases. The kinetics of triggering apo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:Electron-microscope autoradiography has been used to obtain information on the localization of DNA labelled with [3H]thymidine in chloroplasts known to be replicating and concomitantly synthesizing and segregating DNA, in cultured leaf disks. The studies were made using both Microdol-X developer and a 'compact' develo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1976-03-01 00:00:00
abstract：:The sclerotome portion of the differentiating embryonic chick somite becomes infiltrated by neural crest cells prior to its dispersal towards the embryonic axis. This means that sclerotome cells explanted into culture for the purpose of examining their interactions in vitro are contaminated with a proportion of neural...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
abstract：:Cardiac fibrosis is linked to fibroblast-to-myofibroblast phenoconversion and proliferation but the mechanisms underlying this are poorly understood. Ski is a negative regulator of TGF-β-Smad signaling in myofibroblasts, and might redirect the myofibroblast phenotype back to fibroblasts. Meox2 could alter TGF-β-mediat...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.126722
更新日期：2014-01-01 00:00:00
abstract：:In fibroblasts, platelet-derived growth factor receptor alpha (PDGFRα) is upregulated during growth arrest and compartmentalized to the primary cilium. PDGF-AA mediated activation of the dimerized ciliary receptor produces a phosphorylation cascade through the PI3K-AKT and MEK1/2-ERK1/2 pathways leading to the activat...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.116426
更新日期：2013-02-15 00:00:00
abstract：:In the present study, to further elucidate the molecular events that control neural crest cell migration, we have analyzed in vitro the adhesive and locomotory response of avian trunk neural crest cells to laminin-1 and searched for the integrin receptors involved in this process. Adhesion of crest cells on laminin-1 ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:A fundamental prediction of the hypothesis that critical events in tumour formation are rare is that the tumours develop by clonal expansion. This prediction has been tested by establishing the isozyme content of tumours induced in mosaic animals whose normal tissues contain two distinguishable isozyme activities. The...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
abstract：:Desmosomes are intercellular junctions specialised for strong adhesion that are prominent in the epidermis and heart muscle. Defective desmosomal function due to inherited mutations in the constitutive desmosomal gene desmoplakin (DSP) causes skin or heart disorders and in some instances both. Different mutations have...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.084152
更新日期：2012-06-15 00:00:00
abstract：:Excitatory amino acid transporter type 3 (EAAT3, also known as SLC1A1) is a high-affinity, Na(+)-dependent glutamate carrier that localizes primarily within the cell and at the apical plasma membrane. Although previous studies have reported proteins and sequence regions involved in EAAT3 trafficking, the detailed mole...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.185496
更新日期：2016-08-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00808
更新日期：2003-12-01 00:00:00
abstract：:division abnormally delayed (dally) encodes an integral membrane proteoglycan of the glypican family that affects a number of patterning events during both embryonic and larval development. Earlier studies demonstrated that Dally regulates cellular responses to Wingless (Wg) and Decapentaplegic (Dpp) in a tissue-speci...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Mouse oocytes are induced by cytochalasin B to undergo 'pseudo-cleavage' in vitro into 2 equally sized and separable compartments. This response to the drug is dependent upon the meiotic state of the oocytes, as well as upon the presence of an intact zona pellucida. The resulting 2 cellular compartments can be complet...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1976-08-01 00:00:00
abstract：:Cells experience mechanical stresses in different physiological and pathological settings. Clathrin-coated structures (CCSs) are sensitive to such perturbations in a way that often results in a mechanical impairment of endocytic budding. Compressive stress is a mechanical perturbation that leads to increased membrane ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.239681
更新日期：2020-09-01 00:00:00
abstract：:Annexin 1 is a Ca(2+)-regulated membrane binding protein and a major substrate of the epidermal growth factor receptor kinase. Because of its properties and intracellular distribution, the protein has been implicated in endocytic trafficking of the receptor, in particular in receptor sorting occurring in multivesicula...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：:Ezrin/radixin/moesin (ERM) proteins are thought to play an important role in organizing cortical actin-based cytoskeletons through cross-linkage of actin filaments with integral membrane proteins. Recent in vitro biochemical studies have revealed that ERM proteins phosphorylated on their COOH-terminal threonine residu...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
abstract：:Mitotic spindle assembly in animal cells is orchestrated by a chromosome-dependent pathway that directs microtubule stabilization. RanGTP generated at chromosomes releases spindle assembly factors from inhibitory complexes with importins, the nuclear transport factors that facilitate protein import into the nucleus du...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.126854
更新日期：2013-08-01 00:00:00
abstract：:The monoclonal antibody AGF2.3 identifies a nuclear envelope protein that is restricted to certain cell types. In particular, this antigen shows a reduced level of expression during haemopoietic cell maturation. In this study, we have examined the relationship of this protein to known nuclear envelope proteins that ha...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:Sperm fertilization reactions of Limulus polyphemus were examined by scanning electron and/or light microscopy. The following were considered: sperm motility, attachment of sperm to egg, acrosome reaction, and penetration of the acrosomal filament. The spermatozoa after semination are non-motile and become active only...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1976-12-01 00:00:00
abstract：:Hormone secretion by pituitary cells is decreased by roscovitine, an inhibitor of cyclin-dependent kinase 5 (Cdk5). Roscovitine treatment reorganizes cortical actin and ultrastructural analysis demonstrates that roscovitine limits the ability of secretory granules to approach the plasma membrane or one another. Trio, ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01333
更新日期：2004-09-15 00:00:00
abstract：:Double minutes (DMs) seen in a substantial fraction of human tumors are the cytogenetic manifestation of gene amplification which renders the tumor cells advantageous for growth and survival. DMs are acentric and atelomeric chromatin composed of circular DNA. In this study, we found they showed a remarkable relocation...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：:Syndecan-4 is a ubiquitously expressed transmembrane heparan sulphate proteoglycan acting in concert with integrins in the formation of focal adhesions and stress fibres. Signalling events studied thus far suggest the formation of a ternary complex between syndecan-4, phosphatidylinositol 4,5-bisphosphate and protein ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03020
更新日期：2006-07-01 00:00:00
abstract：:The transbilayer dynamics of lipids in the plasma membrane of mammalian sperm cells is crucial for the fertilization process. Here, the transbilayer movement and distribution of phospholipids in the plasma membrane of fresh, ejaculated and cryopreserved ram spermatozoa was studied by labeling cells with fluorescent an...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Endothelial integrity relies on a mechanical crosstalk between intercellular and cell-matrix interactions. This crosstalk is compromised in hemorrhagic vascular lesions of patients carrying loss-of-function mutations in cerebral cavernous malformation (CCM) genes. RhoA/ROCK-dependent cytoskeletal remodeling is central...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.216093
更新日期：2018-08-13 00:00:00
abstract：:Phosphoinositides represent a major class of lipids specifically involved in the organization of signaling cascades, maintenance of the identity of organelles and regulation of multiple intracellular trafficking steps. We previously reported that phosphatidylinositol 5-monophosphate (PI5P), produced by the Shigella fl...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.166314
更新日期：2015-02-15 00:00:00
abstract：:Dystrophic forms of epidermolysis bullosa (DEB) are associated with mutations in the type VII collagen gene (Col7a1) which encodes the major component of anchoring fibrils. To develop a DEB animal model, type VII collagen deficient mice were generated by targeted homologous recombination. The targeting vector replaced...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
abstract：:Mutation of the p53 gene is a common occurrence in human breast cancers but is by no means universal. However, even in tumours where the gene is not mutated altered levels of p53 protein are often detected. This is also observed in cell lines derived from human breast cancers. By transfecting such cell lines containin...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：:The role of inhibition of prelamin A processing in the inhibition of DNA synthesis by lovastatin was examined by expressing prelamin A in F9 teratocarcinoma cells. These cells, normally lacking expression of the A/C lamins, were transfected with constructs expressing either prelamin A or mature lamin A and the effect ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:Several properties of cadherin-4 and cadherin-5 were characterized by using the cDNA transfection approach. The proteins of both cadherins had a relative molecular mass of about 130 kDa and were present at the cell periphery, especially at cell-cell contact sites. These cadherins were easily digested with trypsin, and...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:The effects of isolated plasma membranes on cohesion of Dictyostelium discoideum Ax-2 cells have been studied. Membranes isolated from cells in the log phase of growth gave complete inhibition of log-phase cell cohesion. This effect was specific for the cohesion log-phase cells mediated by contact sites B, since the c...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1979-12-01 00:00:00
