Immunofluorescence detection of ezrin/radixin/moesin (ERM) proteins with their carboxyl-terminal threonine phosphorylated in cultured cells and tissues.

abstract：:The complex formed by Rad9, Rad1 and Hus1 (9-1-1) protects against genomic instability by activating DNA damage checkpoint and DNA damage repair pathways, mainly in response to replication fork collapse and UV lesions. Here we compare the role of Rad9A (also known as Rad9) with the human paralogue Rad9B. Unlike Rad9A,...

journal_title：Journal of cell science

authors： Pérez-Castro AJ,Freire R

更新日期：2012-03-01 00:00:00

abstract：:Accurate chromosome segregation requires the spindle assembly checkpoint to be active at the onset of mitosis, before being silenced following chromosome alignment. p31(comet) is a checkpoint antagonist in that its inhibition delays mitotic exit, whereas its overexpression overrides the checkpoint. How exactly p31(com...

journal_title：Journal of cell science

authors： Westhorpe FG,Tighe A,Lara-Gonzalez P,Taylor SS

更新日期：2011-11-15 00:00:00

abstract：:Integrins play a key role in regulating endothelial cell survival, migration and differentiated function during angiogenic blood-vessel remodeling. Integrin-linked kinase (ILK) is a multidomain protein that interacts with the cytoplasmic tail of integrin beta subunits and is thought to participate in integrin-mediated...

journal_title：Journal of cell science

authors： Vouret-Craviari V,Boulter E,Grall D,Matthews C,Van Obberghen-Schilling E

更新日期：2004-09-01 00:00:00

abstract：:The Arabidopsis AINTEGUMENTA (ANT) gene, which encodes an APETALA2 (AP2)-like transcription factor, controls plant organ cell number and organ size throughout shoot development. ANT is thus a key factor in the development of plant shoots. Here, we have found that ANT plays an essential role in conferring salt toleranc...

journal_title：Journal of cell science

authors： Meng LS,Wang YB,Yao SQ,Liu A

更新日期：2015-08-01 00:00:00

abstract：:Nematocysts are explosive organelles found in all Cnidaria. Explosion of nematocyst capsules is driven by the high pressure within the capsule formed by the high concentration of poly-gamma-glutamate in the capsule matrix. Poly-gamma-glutamate is a polyanion that binds cations tightly, including the fluorescent cation...

journal_title：Journal of cell science

authors： Szczepanek S,Cikala M,David CN

更新日期：2002-02-15 00:00:00

abstract：:The process of angiogenesis requires endothelial cells (ECs) to undergo profound changes in shape and polarity. Although this must involve remodelling of the EC cytoskeleton, little is known about this process or the proteins that control it. We used a co-culture assay of angiogenesis to examine the cytoskeleton of EC...

journal_title：Journal of cell science

authors： Hetheridge C,Scott AN,Swain RK,Copeland JW,Higgs HN,Bicknell R,Mellor H

更新日期：2012-03-15 00:00:00

abstract：:Targeting of glycosylphosphatidylinositol-anchored proteins to the apical surface of epithelial cells involves clustering in Triton X-100-resistant membrane microdomains or rafts. The role of these microdomains in sorting transmembrane proteins is more questionable because, unlike glycosylphosphatidylinositol-anchored...

journal_title：Journal of cell science

authors： Delaunay JL,Breton M,Goding JW,Trugnan G,Maurice M

更新日期：2007-03-15 00:00:00

abstract：:NF-kappaB plays a central role in mediating pathogen and cytokine-stimulated gene transcription. NF-kappaB repressing factor (NRF) has been shown to interact with specific negative regulatory DNA elements (NRE) to mediate transcriptional repression by inhibition of the NF-kappaB activity at certain promoters. mRNA abl...

journal_title：Journal of cell science

authors： Niedick I,Froese N,Oumard A,Mueller PP,Nourbakhsh M,Hauser H,Köster M

更新日期：2004-07-15 00:00:00

abstract：:Integrin-mediated cell adhesion is central to cell survival, differentiation and motility. Many cell responses induced by integrins require both receptor occupancy and receptor aggregation, and appear to be regulated by both biochemical and biophysical means. Multidomain extracellular matrix molecules may serve to fos...

journal_title：Journal of cell science

authors： Koo LY,Irvine DJ,Mayes AM,Lauffenburger DA,Griffith LG

更新日期：2002-04-01 00:00:00

abstract：:Bone erosion both demands that the osteoclast resorbs bone matrix and moves over the bone surface. It is widely accepted that these two activities alternate, because they are considered mutually exclusive since resorption is believed to involve an immobilizing seal to the bone surface. However, clear real-time observa...

journal_title：Journal of cell science

authors： Søe K,Delaissé JM

更新日期：2017-06-15 00:00:00

abstract：:Formation of crossovers between homologous chromosomes during meiosis is positively regulated by the ZMM proteins (also known as SIC proteins). DNA damage checkpoint proteins also promote efficient formation of interhomolog crossovers. Here, we examined, in budding yeast, the meiotic role of the heterotrimeric DNA dam...

journal_title：Journal of cell science

authors： Shinohara M,Hayashihara K,Grubb JT,Bishop DK,Shinohara A

更新日期：2015-04-15 00:00:00

abstract：:Excitatory amino acid transporter type 3 (EAAT3, also known as SLC1A1) is a high-affinity, Na(+)-dependent glutamate carrier that localizes primarily within the cell and at the apical plasma membrane. Although previous studies have reported proteins and sequence regions involved in EAAT3 trafficking, the detailed mole...

journal_title：Journal of cell science

authors： Su JF,Wei J,Li PS,Miao HH,Ma YC,Qu YX,Xu J,Qin J,Li BL,Song BL,Xu ZP,Luo J

更新日期：2016-08-15 00:00:00

abstract：:Mammalian cochlear outer hair cells generate high-frequency forces in response to electrical stimulation. Force generation occurs in the lateral cortex of the cell, which includes the plasma membrane, a two-dimensional 'cortical lattice' of filamentous protein, and a multi-layered membrane system, the lateral cisterna...

journal_title：Journal of cell science

authors： Holley MC,Ashmore JF

更新日期：1990-06-01 00:00:00

abstract：:The mechanism by which glutamate regulates the cytosolic free Ca2+ concentration ([Ca2+]c) in spontaneously firing dopamine neurons is not clear. Thus we have investigated the glutamate-mediated [Ca2+]c dynamics in the acutely isolated dopamine neurons from the rat substantia nigra pars compacta by measuring [Ca2+]c a...

journal_title：Journal of cell science

authors： Choi YM,Kim SH,Uhm DY,Park MK

更新日期：2003-07-01 00:00:00

abstract：:A growing number of proteins originally found in endocytic structures of the plasma membrane appear to be able to traffic into the nucleus, but the cellular function of this translocation remains unclear. We have found that beta-arrestin2, which typically shows a cytoplasmic localization owing to constitutive nuclear ...

journal_title：Journal of cell science

authors： Neuhaus EM,Mashukova A,Barbour J,Wolters D,Hatt H

更新日期：2006-08-01 00:00:00

abstract：:Cancers arising in the oesophageal epithelium are among the most common fatal tumors in the world. Despite this, comparatively little is known about the cell biology and organization of this tissue. Recently, in vitro and in vivo techniques developed over the past 30 years for the study of the epidermis have been appl...

journal_title：Journal of cell science

authors： Seery JP

更新日期：2002-05-01 00:00:00

abstract：:The developmental stage at which Dictyostelium discoideum strain v12/M2 cells was inhibited in resuming cell division was monitored by a Coulter counter. Neither vegetative nor pre-aggregating v12/M2 amoebae used as inocula in liquid growth cultures exhibited a delay in recommencing cell division. Inocula prepared fro...

journal_title：Journal of cell science

authors： Kopachik WJ,Gregg JH

更新日期：1978-02-01 00:00:00

abstract：:Melanotransferrin (p97) is an iron-binding membrane glycoprotein with 40% homology to transferrin and lactoferrin. It was first identified on the basis of its high level of expression in melanoma cells, as compared to normal melanocytes. It is also present in many cultured cell types. In normal tissues, p97 is express...

journal_title：Journal of cell science

authors： Alemany R,Vilá MR,Francí C,Egea G,Real FX,Thomson TM

更新日期：1993-04-01 00:00:00

abstract：:The forkhead transcription factor FOXN1 is required for normal cutaneous and thymic epithelial development. Mutations in FOXN1 give rise to the nude phenotype in mice, rats and man. However, the genes that are regulated by FOXN1 are unknown. To investigate FOXN1 function we expressed an inducible form of the protein, ...

journal_title：Journal of cell science

authors： Janes SM,Ofstad TA,Campbell DH,Watt FM,Prowse DM

更新日期：2004-08-15 00:00:00

abstract：:Schizosaccharomyces pombe cells divide by medial fission throughout contraction of an actomyosin ring and deposition of a multilayered division septum that must be cleaved to release the two daughter cells. Although many studies have focused on the actomoysin ring and septum assembly, little information is available c...

journal_title：Journal of cell science

authors： Martín-Cuadrado AB,Dueñas E,Sipiczki M,Vázquez de Aldana CR,del Rey F

更新日期：2003-05-01 00:00:00

abstract：:The molecular chaperone Hsp90 has been found ubiquitously as a predominantly cytoplasmic dimer. By interacting with cytoplasmic or nuclear proteins such as pp60v-src or steroid receptors, Hsp90 helps its targets to become competent for full biological activity. Mutational deletion analysis of some properties of chicke...

journal_title：Journal of cell science

authors： Meng X,Devin J,Sullivan WP,Toft D,Baulieu EE,Catelli MG

更新日期：1996-07-01 00:00:00

abstract：:The influence of extracellular environmental factors on preimplantation rat blastocysts was tested by determining the number of embryos which escaped from their zonae pellucidae, followed by attachment and outgrowth of trophoblast giant cells, after 72 h in culture Uterine luminal ocmponents from individual females, o...

journal_title：Journal of cell science

authors： Surani MA

更新日期：1977-06-01 00:00:00

abstract：:Different isoforms of non-muscle myosin II have different distributions in vivo, even within individual cells. In order to understand how these different distributions arise, the distribution and dynamics of non-muscle myosins IIA and myosin IIB were examined in cultured cells using immunofluorescence staining and tim...

journal_title：Journal of cell science

authors： Kolega J

更新日期：1998-08-01 00:00:00

abstract：:Activator of G-protein signaling 3 (AGS3, also known as GPSM1) regulates the trans-Golgi network. The AGS3 GoLoco motif binds to Gαi and thereby regulates the transport of proteins to the plasma membrane. Compaction of early embryos is based on the accumulation of E-cadherin (Cdh1) at cell-contacted membranes. However...

journal_title：Journal of cell science

authors： Nie ZW,Niu YJ,Zhou W,Zhou DJ,Kim JY,Cui XS

更新日期：2020-12-07 00:00:00

abstract：:Cilia and flagella are conserved eukaryotic organelles important for motility and sensory. The RanGTPase, best known for nucleocytoplasmic transport functions, may also play a role in protein trafficking into the specialized flagellar/ciliary compartments, although the regulatory mechanisms controlling Ran activity at...

journal_title：Journal of cell science

authors： Brasseur A,Bayat S,Chua XL,Zhang Y,Zhou Q,Low BC,He CY

更新日期：2014-11-15 00:00:00

abstract：:The regulation and function of the crucial cell cycle regulator cyclin E (CycE) remains elusive. Unlike other cyclins, CycE can be uniquely controlled by mitochondrial energetics, the exact mechanism being unclear. Using mammalian cells (in vitro) and Drosophila (in vivo) model systems in parallel, we show that CycE c...

journal_title：Journal of cell science

authors： Parker DJ,Iyer A,Shah S,Moran A,Hjelmeland AB,Basu MK,Liu R,Mitra K

更新日期：2015-11-15 00:00:00

abstract：:Dishevelled-3 (Dvl3) is a multivalent scaffold protein that is essential to Wnt signaling during development. Although Dvl-based punctae have been visualized by fluorescence microscopy; the physical nature and dynamic character of the such complexes are enigmatic. We use steric-exclusion chromatography, affinity pull-...

journal_title：Journal of cell science

authors： Yokoyama N,Golebiewska U,Wang HY,Malbon CC

更新日期：2010-11-01 00:00:00

abstract：:We have isolated the human genes encoding the Pyst1 (MKP-3) and Pyst2 (MKP-X) MAP kinase phosphatases. Both genes consist of three exons interrupted by two introns and lack an intron which is conserved in all the other members of this gene family characterised to date. This reinforces the conclusion that Pyst1 and Pys...

journal_title：Journal of cell science

authors： Dowd S,Sneddon AA,Keyse SM

更新日期：1998-11-01 00:00:00

abstract：:Meckel-Gruber syndrome (MKS) is a severe autosomal recessively inherited disorder caused by mutations in genes that encode components of the primary cilium and basal body. Here we show that two MKS proteins, MKS1 and meckelin, that are required for centrosome migration and ciliogenesis interact with actin-binding isof...

journal_title：Journal of cell science

authors： Dawe HR,Adams M,Wheway G,Szymanska K,Logan CV,Noegel AA,Gull K,Johnson CA

更新日期：2009-08-01 00:00:00

abstract：:The GGGGCC (G4C2) repeat expansion mutation in the C9ORF72 gene is the most common genetic cause of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). Transcription of the repeat and formation of nuclear RNA foci, which sequester specific RNA-binding proteins, is one of the possible pathological me...

journal_title：Journal of cell science

authors： Bajc Česnik A,Darovic S,Prpar Mihevc S,Štalekar M,Malnar M,Motaln H,Lee YB,Mazej J,Pohleven J,Grosch M,Modic M,Fonovič M,Turk B,Drukker M,Shaw CE,Rogelj B

更新日期：2019-03-07 00:00:00