Abstract:
:The influence of extracellular environmental factors on preimplantation rat blastocysts was tested by determining the number of embryos which escaped from their zonae pellucidae, followed by attachment and outgrowth of trophoblast giant cells, after 72 h in culture Uterine luminal ocmponents from individual females, or hormones, were included in Dulbecco's medium which contained 4 mg/ml bovine serum albumin. In about 20% of cases, uterine fluids were embryotonic. However, uterine fluids from day-5 pregnant females, the day of implantation in the rat, were more potent in these tests than uterine fluids obtained from ovariectomized females treated with progesterone alone. The potency of a mixture of the 2 fluids was also high. Uterine fluids obtained at 14 h after an injection of oestradiol and progesterone to the ovariectomized females, were also effective in these tests. Rat serum and foetal calf serum were effective too, but steroids or insulin alone in the medium had no detectable influence on embryos. Serum or uterine luminal proteins appear to be essential for maintaining the viability of the blastocysts and for inducing the responses observed here. In the uterine fluids, some proteins released into the lumen after treatment of females with oestradiol and progesterone appear to be the biologically active components. Differences in the responses of blastocysts in vitro are compared with those in vivo.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Surani MAsubject
Has Abstractpub_date
1977-06-01 00:00:00pages
265-77eissn
0021-9533issn
1477-9137journal_volume
25pub_type
杂志文章abstract::The effects of two inhibitors of 3-hydroxy 3-methyl glutaryl-coenzyme A reductase (tunicamycin and mevinolin) on nuclear division in the myxomycete Physarum polycephalum were examined. Tunicamycin exerted a minor effect on division in synchronized cultures, whereas mevinolin delayed the second, third and fourth nuclea...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1989-03-01 00:00:00
abstract::Endocytosis is characterized by vesicular transport along numerous pathways. Common steps in each pathway include membrane budding to form vesicles, transport to a particular destination, and ultimately docking and fusion with the target membrane. Specificity of vesicle targeting is rendered in part by associated Rab ...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
abstract::Receptor protein tyrosine phosphatase beta (RPTP beta) mediates cell-cell and cell-matrix interactions. By searching for intracellular proteins that interact with the cytoplasmic region of this phosphatase using the two-hybrid method, we identified several proteins containing PDZ domains. One of these proteins, MAGI-3...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00302
更新日期:2003-04-01 00:00:00
abstract::In the developing Drosophila eye, the centrioles of the differentiating retinal cells are not surrounded by the microtubule-nucleating γ-tubulin, suggesting that they are unable to organize functional microtubule-organizing centers. Consistent with this idea, Cnn and Spd-2, which are involved in γ-tubulin recruitment,...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.211441
更新日期:2018-02-22 00:00:00
abstract::Putative protein synthesizing domains, called plaques, are characterized in the squid giant synapse and axon and in terminals of squid photoreceptor neurons. Plaques are oval-shaped formations of about 1 microm in size, which (1) generate signals that have spectroscopic electron energy loss characteristics of ribosome...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::Calmodulin was localized in the small intestine of Necturus maculosa at both the light- and electron-microscopic levels utilizing an affinity-purified, monospecific antibody and immunoperoxidase cytochemistry. The microvilli and basolateral membranes of the epithelium were highly reactive, the cytoplasm was moderately...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::Onconase is an RNase with a very specific property because it is selectively toxic to transformed cells. This toxin is thought to recognize cell surface receptors, and the protection conferred by metabolic poisons against Onconase toxicity indicated that this RNase relies on endocytic uptake to kill cells. Nevertheles...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03427
更新日期:2007-04-15 00:00:00
abstract::A universal feature of mitosis is that all chromosomes become aligned at the spindle equator--the halfway point between the two spindle poles--prior to anaphase onset. This migratory event is called congression, and is powered by centromere-bound protein machines called kinetochores. This Commentary aims to document r...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.169367
更新日期:2015-09-15 00:00:00
abstract::Atomic force microscopy (AFM) was used to probe topology, conformational changes and initial substrate-carrier interactions of Na+-glucose co-transporter (SGLT1) in living cells on a single-molecule level. By scanning SGLT1-transfected Chinese hamster ovary (CHO) cells with AFM tips carrying an epitope-specific antibo...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03035
更新日期:2006-07-15 00:00:00
abstract::Polyethylene glycol (PEG) was used to fuse trout sperm with Chinese hamster fibroblasts (CHW-1102). As judged by light microscopy, PEG significantly increased the number of associations between the two cell types. Electron microscopy revealed that the sperm nuclei were in the cytoplasm of CHW-1102 in cultures that had...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
abstract::This study examined the role of Ca(2+) in regulatory volume decrease by Necturus erythrocytes. Hypotonic shock (50% tonicity) stimulated an increase in cytosolic free Ca(2+), detected using epi-fluorescence microscopy and the fluorescent Ca(2+) indicator fluo-4-AM (10 microM). A similar increase in fluorescence did no...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00202
更新日期:2003-01-01 00:00:00
abstract::It is becoming clear that mechanical stimuli are crucial factors in regulating the biology of the cell, but the short-term structural response of a cell to mechanical forces remains relatively poorly understood. We mechanically stimulated cells transiently expressing actin-EGFP with controlled forces (0-20 nN) in orde...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.088302
更新日期:2012-02-01 00:00:00
abstract::Human SW-13 cells express the intermediate filament protein vimentin in a mosaic pattern (Hedberg, K. K. and Chen, L. B. (1986). Exp. Cell Res. 163, 509-517). We have isolated SW-13 clones that do (vim+) or do not (vim-) synthesize vimentin as analyzed using anti-intermediate filament immunofluorescence, electron micr...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::Pharmacological inhibitors of JNK (SP600125) and p38 (PD169316) sensitize tumor cells to Fas-mediated apoptosis. PD169316 is less potent than SP600125 and diminishes its effect when present together. Because the p38 isoforms that promote (p38alpha) or inhibit (p38beta) apoptosis are both suppressed by PD169316, we inv...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01573
更新日期:2004-12-15 00:00:00
abstract::During pre-mRNA splicing, introns are removed and exons are ligated to form an mRNA. Exon choice is determined by different nuclear protein concentrations varying among tissues and cell types or by developmental stage. These can be altered by different cellular circumstances such as physiological stimuli, environmenta...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01720
更新日期:2005-03-15 00:00:00
abstract::As chromosomes move polewards during anaphase in crane-fly spermatocytes, trailing arms commonly stretch backwards for a brief time, as if tethered to their partners. To test that notion, a laser microbeam was used to sever trailing arms and thereby release telomere-containing arm segments (called acentric fragments b...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-04-01 00:00:00
abstract::We have cloned and characterized a single copy C. reinhardtii gene containing an open reading frame of 333 nucleotides encoding a 12.7 kDa protein. The novel protein, DIP13, exhibits 60% identity with two mammalian proteins, human NA14 and an unnamed mouse protein. Homologous sequences are also present in several prot...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00337
更新日期:2003-04-15 00:00:00
abstract::A growing number of proteins originally found in endocytic structures of the plasma membrane appear to be able to traffic into the nucleus, but the cellular function of this translocation remains unclear. We have found that beta-arrestin2, which typically shows a cytoplasmic localization owing to constitutive nuclear ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03046
更新日期:2006-08-01 00:00:00
abstract::MicroRNAs are known to play regulatory roles in gene expression associated with cancer development. We analyzed levels of the microRNA miR-24 in patients with breast carcinoma and found that miR-24 was higher in breast carcinoma samples than in benign breast tissues. We generated constructs expressing miR-24 and studi...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.118299
更新日期:2013-03-15 00:00:00
abstract::Dynamins are a conserved family of proteins involved in membrane fusion and fission. Although mammalian dynamins are known to be involved in several membrane-trafficking events, the role of dynamin-1 in endocytosis is the best-characterised role of this protein family. Despite many similarities between endocytosis in ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.070508
更新日期:2010-10-15 00:00:00
abstract::Recent evidence suggests that organization of the extracellular matrix (ECM) into aligned fibrils or fibril-like ECM topographies promotes rapid migration in fibroblasts. However, the mechanisms of cell migration that are altered by these changes in micro-environmental topography remain unknown. Here, using 1D fibrill...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.098806
更新日期:2012-05-01 00:00:00
abstract::Fluorescence in situ hybridization has demonstrated that chromosomes form individual territories in interphase nuclei. However, this technique is not suitable to determine whether territories are mutually exclusive or interwoven. This notion, however, is essential for understanding functional organizations in the cell...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::The ret finger protein (rfp) is a member of the B box zinc finger gene family which possesses a tripartite motif consisting of a RING finger, B box finger, and a coiled-coil domain. Rfp is expressed at specific stages of spermatogenesis and in various adult mouse and human tissues. It becomes oncogenic when the tripar...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract::Epitectin, the mucin-like glycoprotein defined by the monoclonal antibodies CA1, CA2 and CA3, has been examined by electron microscopy to determine its shape and size. It appears to be a single extended strand with a mean length of about 270 nm. The antibodies CA1 and CA2 appear to bind preferentially to a terminal si...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
abstract::We investigated human Hap50, the large isoform of the previously characterized Hsp70/Hsc70-associating protein Hap46, also called BAG-1, for effects on transcriptional activities. Overproduction by transient transfection led to enhanced expression of reporter gene constructs in various cell types using different promo...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00
abstract::Many adherent cells in vitro are surrounded by a transparent exclusion zone or halo, several micrometers thick, which red blood cells, bacteria and carbon particles cannot penetrate. This halo is rapidly and specifically removed by hyaluronidase and its high degree of hydration is demonstrated by the fact that, althou...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1983-07-01 00:00:00
abstract::Human peripheral blood eosinophils attach to and flatten down onto antibody-coated surfaces and subsequently degranulate. An antibody-coated surface was prepared by treating a layer of agar, containing tetanus toxoid antigen and eosinophil chemotactic factor (ECF), with human anti-tetanus immunoglobin. Changes in eosi...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1982-08-01 00:00:00
abstract::BCL6 is a potent transcriptional repressor that plays important roles in germinal center formation, T helper cell differentiation and lymphomagenesis and regulates expression of several chemokine genes in macrophages. In a further investigation of its role in macrophages, we show that BCL6 inactivation in primary bone...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02314
更新日期:2005-05-01 00:00:00
abstract::A scanning electron-microscope study has established that there are major modifications in the surface membranes of malignant cells transformed from the pronephric kidney of the frog. Tumours were induced in vivo after exposure of embryos Lucké Herpes virus. A pronephric cell line from normal embryos and 3 tumour cell...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract::Exocytotic incorporation of plasmalemmal precursor vesicles (PPVs) into the cell surface is necessary for axonal outgrowth and is known to occur mainly at the nerve growth cone. We have demonstrated recently that plasmalemmal expansion is regulated at the growth cone by IGF-1, but not by BDNF, in a manner that is quas...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02490
更新日期:2005-08-15 00:00:00