Abstract：
:During pre-mRNA splicing, introns are removed and exons are ligated to form an mRNA. Exon choice is determined by different nuclear protein concentrations varying among tissues and cell types or by developmental stage. These can be altered by different cellular circumstances such as physiological stimuli, environmental effects and phosphorylation state. The splicing factor hSlu7 plays an important role in 3' splice site selection during the second step of splicing in vitro and has been suggested to affect alternative splicing in vivo. Our results indicate that an ultraviolet-C (UV-C) stress stimulus triggers changes in the alternative splicing patterns of cellular genes by decreasing the nuclear concentration of hSlu7 through the modulation of its nucleus-to-cytoplasm transport. This shift is mostly dependent on the Jun N-terminal kinase (JNK) cascade. Although we found by RNAi knockdown that hSlu7 is not essential for cell viability, its nuclear concentration effects exon choice and inclusion:skipping ratio of alternative splicing. A possible spatial and temporal regulatory mechanism by which hSlu7 protein levels are regulated within the nucleus is suggested, thus implying a broad effect of hSlu7 on alternative splicing.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Shomron N,Alberstein M,Reznik M,Ast Gdoi
10.1242/jcs.01720keywords:
subject
Has Abstractpub_date
2005-03-15 00:00:00pages
1151-9issue
Pt 6eissn
0021-9533issn
1477-9137pii
jcs.01720journal_volume
118pub_type
杂志文章abstract：:Fibroblast migration is an integral component of biological processes such as wound healing and embryogenesis. Previous experiments examining fibroblast locomotion from tissue explants have shown that migrating fibroblasts lack, or contain only transient, focal adhesions (focal contacts). Focal adhesions are specializ...
journal_title：Journal of cell science
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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更新日期：1987-02-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2002-05-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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更新日期：2017-07-01 00:00:00
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更新日期：2005-09-15 00:00:00
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journal_title：Journal of cell science
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更新日期：2004-05-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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更新日期：2018-06-06 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00918
更新日期：2003-12-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.021394
更新日期：2008-07-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1980-10-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.213462
更新日期：2018-05-04 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.145565
更新日期：2014-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03086
更新日期：2006-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-08-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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doi：
更新日期：2000-04-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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更新日期：2004-05-15 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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