Abstract:
:Receptor protein tyrosine phosphatase beta (RPTP beta) mediates cell-cell and cell-matrix interactions. By searching for intracellular proteins that interact with the cytoplasmic region of this phosphatase using the two-hybrid method, we identified several proteins containing PDZ domains. One of these proteins, MAGI-3, contains a guanylate-kinase-like region, six PDZ and two WW domains. The interaction between RPTP beta and MAGI-3 was confirmed by co-immunoprecipitation and pulldown experiments in transfected cells. Immunofluorescence and immunoelectron microscopy revealed that MAGI-3 is concentrated in specific sites at the plasma membrane and in the nucleus. In epithelial cells, MAGI-3 was localized with ZO-1 and cingulin at tight junctions, whereas in primary cultured astrocytes it was found in E-cadherin-based cell-cell contacts and in focal adhesion sites. Although MAGI-3 itself was not phosphorylated on tyrosine residues, it became associated with tyrosine-phosphorylated proteins following a short treatment of the cells with vanadate. In glioblastoma SF763T cells MAGI-3 was associated with a tyrosine-phosphorylated protein with the apparent molecular weight of 130 kDa, whereas in Caco2 cells it was associated with a 90 kDa protein. Finally, we show that p130 served as a substrate for RPTP beta and that its dephosphorylation required the C-terminal sequence of the phosphatase, which mediated the interaction with MAGI-3. These findings suggest a possible role for MAGI-3 as a scaffolding molecule that links receptor tyrosine phosphatase with its substrates at the plasma membrane.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Adamsky K,Arnold K,Sabanay H,Peles Edoi
10.1242/jcs.00302keywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
1279-89issue
Pt 7eissn
0021-9533issn
1477-9137journal_volume
116pub_type
杂志文章abstract::SNARE (Soluble N-ethyl-maleimide sensitive factor Attachment protein Receptor) proteins assemble in tight core complexes, which promote fusion of carrier vesicles with target compartments. Members of this class of proteins are expressed in all eukaryotic cells and are distributed in distinct subcellular compartments. ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::Following fertilization, there are rapid changes in the appearance of the Fucus egg. Large electron-translucent vesicles (V1) accumulate fibrillar material, and following pronuclear fusion, they are largely electron-opaque. These vesicles (V1) are formed originally in unfertilized eggs by smooth endoplasmic reticulum ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-03-01 00:00:00
abstract::Mesenchyme fragments from early wing buds of normal and talpid3 mutant chick embryos were explanted for culture in plastic Petri dishes and the behaviour of individual cells as they moved out on to the plastic surface was studied by time-lapse ciné photography, followed by statistical analysis. Two parameters of cell ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1975-07-01 00:00:00
abstract::Activity of AURKA is controlled through multiple mechanisms including phosphorylation, ubiquitin-mediated degradation and allosteric interaction with TPX2. Activity peaks at mitosis, before AURKA is degraded during and after mitotic exit in a process strictly dependent on the APC/C coactivator FZR1. We used FZR1 knock...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.243071
更新日期:2020-06-16 00:00:00
abstract::Xenopus embryos initiate replication at random closely spaced sites until a certain concentration of nuclei is achieved within the embryo, after which fewer, more specific chromosomal sites are utilized as origins. We have examined the relationship between nucleo-cytosolic ratio and origin specification when Chinese h...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract::In order to produce proteins essential for their propagation, many pathogenic human viruses, including SARS-CoV-2 the causative agent of COVID-19 respiratory disease, commandeer host biosynthetic machineries and mechanisms. Three major structural proteins, the spike, envelope and membrane proteins, are amongst several...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.257758
更新日期:2021-01-19 00:00:00
abstract::Neutrophil leucocytes circulate in plasma, which contains broad-spectrum antiproteases such as alpha-2-macroglobulin and alpha-1-antitrypsin, and move into environments in which antiproteases are present. Plasma antiproteases significantly reduce the adhesiveness of leucocytes in both static and dynamic adhesion assay...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::Eukaryotic flagella undertake different beat types as necessary for different functions; for example, the Leishmania parasite flagellum undergoes a symmetric tip-to-base beat for forward swimming and an asymmetric base-to-tip beat to rotate the cell. In multi-ciliated tissues or organisms, the asymmetric beats are coo...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.246637
更新日期:2020-10-22 00:00:00
abstract::Nemaline myopathy (NM) is a congenital myopathy with an estimated incidence of 150,000 live births. It is caused by mutations in thin filament components, including nebulin, which accounts for about 50% of the cases. The identification of NM cases with nonsense mutations resulting in loss of the extreme C-terminal SH3...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.137026
更新日期:2013-12-01 00:00:00
abstract::DNA replication in eukaryotic cells initiates from multiple replication origins that are distributed throughout the genome. Coordinating the usage of these origins is crucial to ensure complete and timely replication of the entire genome precisely once in each cell cycle. Replication origins fire according to a cell-t...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.096701
更新日期:2013-03-15 00:00:00
abstract::Previous work has shown that phorbol esters or diacylglycerol trigger cortical granule exocytosis in Xenopus oocytes. We sought to identify the isoform(s) of protein kinase C (PKC) that mediate(s) this regulated secretory event. Because this process is initiated by lipid activators of PKC but is independent of calcium...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-03-15 00:00:00
abstract::The presence of gap junctions in stratified epithelia has now been extensively documented, but there have been few attempts to quantify them. In the present report, samples of hamster cheek pouch mucosa were processed for electron microscopy and electron micrographs from defined basal, spinous and granular layers were...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00
abstract::The Wnt-5a gene encodes a secreted protein that controls several normal processes during embryogenesis and development of adult tissues by as yet unknown mechanisms. Endogenous expression of Wnt-5a mRNA is known to occur in both mouse and human mammary cell lines. To investigate the biological role of Wnt-5a in the hu...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-06-01 00:00:00
abstract::Previously, we have identified a class of retinal degeneration mutants in Drosophila in which the normally transient interaction between arrestin2 (Arr2) and rhodopsin is stabilized and the complexes are rapidly internalized into the cell body by receptor-mediated endocytosis. The accumulation of protein complexes in ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03052
更新日期:2006-08-01 00:00:00
abstract::Thrombospondin-1 (TSP-1) and the highly related protein thrombospondin-2 (TSP-2) are trimeric extracellular molecules that have complex roles in wound healing, angiogenesis and matrix organisation. At the cellular level, TSP-1 supports cell adhesion and migration by the organisation of fascin spike cytoskeletal struct...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract::Integrins mediate cell adhesion and signal transduction at focal adhesions. Here we investigate the roles of integrin beta subunits in the regulation of actin cytoskeletal structure and the activities of Rho and Rac. The overexpression of beta3 integrin in Chinese hamster ovary cells enhances Rho activity and stress f...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-05-15 00:00:00
abstract::Fluorescence in situ hybridization in combination with synaptonemal complex and spindle pole body immunostaining to both spread and structurally preserved nuclei from time course experiments disclosed prominent telomere clustering during meiotic prophase of the yeast Saccharomyces cerevisiae. It was found that centrom...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract::The 16 kDa proteolipid (subunit c) of the eukaryotic vacuolar H(+)-ATPase (V-ATPase) is closely related to the ductin polypeptide that forms the connexon channel of gap junctions in the crustacean Nephrops norvegicus. Here we show that the major protein component of Manduca sexta gap junction preparations is a 16 kDa ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-07-01 00:00:00
abstract::Neural stem cells (NSCs) typically show efficient self-renewal and selective differentiation. Their invasion potential, however, is not well studied. In this study, Sox2-positive NSCs from the E14.5 rat cortex were found to be non-invasive and showed only limited migration in vitro. By contrast, FGF2-expanded NSCs sho...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.125757
更新日期:2013-08-15 00:00:00
abstract::The coordinated cellular responses to physiological stress are known to be effected in part by the activation of heat-shock factor 1, a transcriptional activator protein capable of binding to, and inducing transcription from genes containing heat shock elements. Other stress responsive signal transduction pathways als...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
abstract::The cytoplasmic pH of growing pollen tubes of Lilium longiflorum Thunb. was measured using the pH-sensitive fluorescent dye 2',7'-bis-(carboxyethyl)-5(6')-carboxyfl uorescein and confocal fluorescence ratio imaging. The average cytoplasmic pH in the clear zone of the pollen tube tip was pH 7.11, and no consistent pH g...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00
abstract::In invertebrates, UNC-45 regulates myosin stability and functions. Vertebrates have two distinct isoforms of the protein: UNC-45B, expressed in muscle cells only, and UNC-45A, expressed in all cells and implicated in regulating both non-muscle myosin II (NMII)- and microtubule (MT)-associated functions. Here, we show ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.248815
更新日期:2021-01-08 00:00:00
abstract::Several studies have shown that altered gravity conditions influence mammalian cell growth and differentiation. The molecular mechanisms underlying these effects, however, remain relatively obscure. In this paper we show that microgravity reached in a sounding rocket strongly decreases epidermal growth factor (EGF)-in...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
abstract::T cell activation is accompanied by actin-mediated receptor clustering and reorganization of lipid rafts. It has been suggested that costimulatory molecules might be involved in these processes. We here provide evidence that engagement of the adhesion molecule CD44 initiates cytoskeletal rearrangement and membrane reo...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::Postsynaptic density protein 95 (PSD-95/SAP-90) is a palmitoylated membrane-associated guanylate kinase that oligomerizes and clusters ion channels and associated signaling machinery at excitatory synapses in brain. However, the mechanism for PSD-95 oligomerization and its relationship to ion channel clustering remain...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00617
更新日期:2003-08-01 00:00:00
abstract::Membrane-bound transferrin-like protein (MTf), a glycosylphosphatidylinositol-anchored protein, is expressed at high levels in many tumors and in several fetal and adult tissues including cartilage and the intestine, as well as in the amyloid plaques of Alzheimer's disease, although its role remains unknown. MTf is on...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00393
更新日期:2003-05-15 00:00:00
abstract::Understanding the interactions between distinct epithelial cells would help us to understand the development of tissues. Drosophila imaginal discs, which are made up of two types of epithelial cells, provide good model systems for such studies. The disc proper or the columnar epithelial cells are apposed to a layer of...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02464
更新日期:2005-08-01 00:00:00
abstract::The conformation and biological activities of fibronectin (FN) and vitronectin (VN) coated on different plastic surfaces were investigated using cell adhesion and a panel of domain-specific monoclonal antibodies (mAbs). The adhesion of BHK fibroblasts was markedly better on FN coated on hydrophilic tissue culture poly...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract::Extracellular matrix receptors of the integrin family have a dual role in the epidermis, regulating both adhesion and differentiation. Loss of contact with the extracellular matrix causes keratinocytes to become committed to terminal differentiation, and results in a decrease in the ability of the alpha 5 beta 1 integ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::Electrical synapses (gap junctions) rapidly transmit signals between neurons and are composed of connexins. In neurons, connexin36 (Cx36) is the most abundant isoform; however, the mechanisms underlying formation of Cx36-containing electrical synapses are unknown. We focus on homocellular and heterocellular gap juncti...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.133066
更新日期:2014-03-15 00:00:00