Abstract:
:The 16 kDa proteolipid (subunit c) of the eukaryotic vacuolar H(+)-ATPase (V-ATPase) is closely related to the ductin polypeptide that forms the connexon channel of gap junctions in the crustacean Nephrops norvegicus. Here we show that the major protein component of Manduca sexta gap junction preparations is a 16 kDa polypeptide whose N-terminal sequence is homologous to ductin and is identical to the deduced sequence of a previously cloned cDNA from Manduca (Dow et al., Gene, 122, 355-360, 1992). We also show that a Drosophila melanogaster cDNA, highly homologous to the Manduca cDNA, can rescue Saccharomyces cerevisiae, defective in V-ATPase function, in which the corresponding yeast gene, VMA3, has been inactivated. Evidence is presented for a single genetic locus (Vha16) in Drosophila, which in adults at least contains a single transcriptional unit. Taken together, the data suggest that in Drosophila and Manduca, the same polypeptide is both the proteolipid subunit c component of the V-ATPase and the ductin component of gap junctions. The intron/exon structure of the Drosophila Vha16 is identical to that of a human Vha16 gene, and is consistent with an ancient duplication of an 8 kDa domain. A pilot study for gene inactivation shows that transposable P-elements can be easily inserted into the Drosophila ductin Vha16 gene. Although without phenotypic consequences, these can serve as a starting point for generation of null alleles.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Finbow ME,Goodwin SF,Meagher L,Lane NJ,Keen J,Findlay JB,Kaiser Ksubject
Has Abstractpub_date
1994-07-01 00:00:00pages
1817-24eissn
0021-9533issn
1477-9137journal_volume
107 ( Pt 7)pub_type
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1999-03-01 00:00:00
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更新日期:2001-01-01 00:00:00
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2005-01-15 00:00:00
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journal_title:Journal of cell science
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更新日期:1989-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-06-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1997-03-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1997-10-01 00:00:00
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更新日期:1977-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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