Abstract:
:We analysed the phenotypic outcome of a Stra8-null mutation on male meiosis. Because the mutant spermatocytes (1) underwent premeiotic DNA replication, (2) displayed cytological features attesting initiation of recombination and of axial-element assembly, and (3) expressed Spo11 and numerous other meiotic genes, it was concluded that STRA8 is dispensable for meiotic initiation. The few mutant spermatocytes that progressed beyond leptonema showed a prolonged bouquet-stage configuration, asynapsis and heterosynapsis, suggesting function(s) of STRA8 in chromosome pairing. Most importantly, a large number of mutant leptotene spermatocytes underwent premature chromosome condensation, within 24 hours following the meiotic S phase. This phenomenon yielded aberrant metaphase-like cells with 40 univalent chromosomes, similar to normal mitotic metaphases. From these latter observations and from the wild-type pattern of Stra8 expression, we propose that, in preleptotene spermatocytes, STRA8 is involved in the process that leads to stable commitment to the meiotic cell cycle.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Mark M,Jacobs H,Oulad-Abdelghani M,Dennefeld C,Féret B,Vernet N,Codreanu CA,Chambon P,Ghyselinck NBdoi
10.1242/jcs.035071subject
Has Abstractpub_date
2008-10-01 00:00:00pages
3233-42issue
Pt 19eissn
0021-9533issn
1477-9137pii
121/19/3233journal_volume
121pub_type
杂志文章abstract::Nuclear sap, on fixation in glutaraldehyde, forms a fibrous network that resembles chromatin in its dimensions and staining properties. This artifactual network is easily confused with true chromatin fibres in sections of nuclei. With formaldehyde a homogeneous array of beads is produced. These are approximately 10 nm...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1977-08-01 00:00:00
abstract::This study describes the functional characterization of two cell lines derived from the proximal convoluted (PKSV-PCT cells) and proximal straight (PKSV-PR) tubules microdissected out from kidneys of transgenic mice harboring the simian virus 40 (SV40) large T and small t antigens placed under the control of the rat L...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract::Triosephosphate isomerase (TPI) is a glycolytic enzyme that converts dihydroxyacetone phosphate (DHAP) into glyceraldehyde 3-phosphate (GAP). Glycolytic enzyme dysfunction leads to metabolic diseases collectively known as glycolytic enzymopathies. Of these enzymopathies, TPI deficiency is unique in the severity of neu...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.124586
更新日期:2013-07-15 00:00:00
abstract::Dynamin, a microtubule-activated GTPase, has recently been identified as the product of the shibire gene in Drosophila. shi(ts) mutants are defective in synaptic vesicle recycling, which leads to rapid and reversible temperature-sensitive paralysis. In the present study, results from RNase protection assays and analys...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::In this study we describe DNA-RNA complexes in matrix DNA of Friend cells. The presence of such unusual structures is confirmed by the following evidence. When a preparation of matrix DNA is electrophoresed in agarose an RNA component always migrates together with DNA. There should be a close interaction between DNA a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract::NEDD8 is an important regulatory factor in many biological processes. However, the substrates for neddylation, and the relationship between the ubiquitin and NEDD8 pathways remain largely unknown. Here, we show that NEDD8 is covalently conjugated to histone 2A (H2A), and that neddylation of H2A antagonizes its ubiquit...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.138891
更新日期:2014-05-15 00:00:00
abstract::The neuron specific Drosophila ELAV protein belongs to the ELAV family of RNA binding proteins which are characterized by three highly conserved RNA recognition motifs, an N-terminal domain, and a hinge region between the second and third RNA recognition motifs. Despite their highly conserved RNA recognition motifs th...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Previous work from this laboratory has indicated that very low concentrations of vinblastine block HeLa cells at mitosis in the presence of a full complement of microtubules and without major disruption of spindle organization. In the present study we analyzed the structural organization of mitotic spindle microtubule...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::The subnuclear organization of nucleic acids and cognate regulatory factors suggests that there are functional interrelationships between nuclear structure and gene expression. Nuclear proteins that are localized in discrete domains within the nucleus include the leukemia-associated acute myelogenous leukemia (AML) an...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:
更新日期:2000-07-01 00:00:00
abstract::In plants, many nucleus-encoded proteins are targeted to both mitochondria and plastids, and this process is generally mediated by ambiguous N-terminal targeting sequences that are recognized by receptors on both organelles. In many algae, however, plastids were acquired by secondarily engulfing green or red algae, wh...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.116533
更新日期:2012-12-15 00:00:00
abstract::To clarify the involvement of actin in the formation of the yeast cell wall, reverting protoplasts of Schizosaccharomyces pombe were used as a simple model system. Actin of reverting protoplasts was labeled with rhodamine-conjugated phalloidin and observed by conventional fluorescence microscopy and laser scanning con...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
abstract::The lymphatic endothelial cell (LEC) fate decision program during development has been described. However, the mechanism underlying the maintenance of differentiated LEC identity remains largely unknown. Here, we show that fibroblast growth factor 2 (FGF2) plays a fundamental role in maintaining a differentiated LEC t...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.137836
更新日期:2014-02-15 00:00:00
abstract::The protozoan parasite Giardia lamblia has the unusual morphology of bearing two equal-sized nuclei. This organism probably represents the earliest diverging lineage of eukaryotes, suggesting that its biological tactics may be transitional. To begin to understand the role played by the two equal-sized nuclei in this o...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00
abstract::Adaptors are the membrane-binding components of clathrin-coated vesicles. The interaction of the trans-Golgi coat adaptor AP1 with membrane-associated proteins was analyzed by affinity chromatography. Proteins of 83 and 52 kDa bound specifically to the core domain of AP1 and showed no interaction with AP2 or other cla...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::A cDNA encoding rat CaBP1 has been isolated and sequenced. The deduced polypeptide chain consists of 440 amino acids including two internal thioredoxin-like domains and a C-terminal KDEL retention/retrieval signal. Regarding the high degree of identity to the hamster protein P5, CaBP1 is considered to be the homologou...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::Disruption of the sarcoglycan complex leads to muscle membrane instability and muscular dystrophy in humans and mice. Through the dystrophin glycoprotein complex, sarcoglycan participates in connecting the internal cytoskeleton to the membrane and the extracellular matrix. Integrin alpha7beta1 is also a transmembrane ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01234
更新日期:2004-08-01 00:00:00
abstract::In culture, cell confluence generates signals that commit actively growing keratinocytes to exit the cell cycle and differentiate to form a stratified epithelium. Using a comparative proteomic approach, we studied this 'confluence switch' and identified a new pathway triggered by cell confluence that regulates basemen...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.096289
更新日期:2012-09-15 00:00:00
abstract::Candida albicans is a dimorphic fungus capable of growing as a budding yeast and as a filamentous hypha. We have used the technique of immunofluorescence to study the changes in the microtubule cytoskeleton during the cell cycle in both growth forms. This approach has revealed the presence of an extensive system of mi...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::The core protease (CP) subcomplex of the 26S proteasome houses the proteolytic active sites and assumes a barrel shape comprised of four co-axially stacked heptameric rings formed by structurally related α- and β-subunits. CP biogenesis typically begins with the assembly of the α-ring, which then provides a template f...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.249862
更新日期:2020-11-03 00:00:00
abstract::Hormone secretion by pituitary cells is decreased by roscovitine, an inhibitor of cyclin-dependent kinase 5 (Cdk5). Roscovitine treatment reorganizes cortical actin and ultrastructural analysis demonstrates that roscovitine limits the ability of secretory granules to approach the plasma membrane or one another. Trio, ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01333
更新日期:2004-09-15 00:00:00
abstract::The invasiveness of mouse lymphocytes and thymocytes, rabbit peritoneal neutrophil granulocytes (PMNs), mouse peritoneal macrophages (both activated and non-activated) and pig endothelial cells was assayed by implanting these cells to the chick wing bud. Cells of each type moved into the wing mesenchyme, although acti...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-12-01 00:00:00
abstract::Semaphorin 3A (Sema3A), a secretory semaphorin, exerts various biological actions through a complex between neuropilin-1 and plexin-As (PlexAs). Sema3A induces retrograde signaling, which is involved in regulating dendritic localization of GluA2 (also known as GRIA2), an AMPA receptor subunit. Here, we investigated a ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.184580
更新日期:2016-05-01 00:00:00
abstract::We have found that Ehrlich ascites tumour (EAT) cells, deprived of any carbon source, and suspended at a density of 2 X 10(5) cells/cm3, begin to die only after 12 h of starvation, though it is known that under these conditions they lose over 80% of their ATP within 30 min. Moreover, we have found that the viability o...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-08-01 00:00:00
abstract::The elliptical, anucleate erythrocytes of camels have been examined for the presence of marginal bands and their constituent microtubules. Lysis of erythrocytes under microtubule-stabilizing conditions readily revealed marginal bands in at least 3 % of the cells, as observed by phase-contrast and darkfield light micro...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-04-01 00:00:00
abstract::The chemokine CCL2 serves to target circulating monocytes and other leukocytes to tissue during innate immune responses, and modulates the progression of chronic inflammatory disease through activation of the receptor CCR2. Here, we show that co-activation of the P2Y₆ purinergic receptor (encoded by P2RY₆) occurs when...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.159012
更新日期:2014-11-15 00:00:00
abstract::Clathrin-mediated endocytosis requires cargo-specific adaptor proteins that recognize specific receptors and recruit them into coated pits. ARH [also called low-density lipoprotein receptor (LDLR) adaptor protein] serves as an adaptor for LDLR endocytosis in liver. However, ARH is dispensable for LDL uptake by some ot...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03217
更新日期:2006-10-15 00:00:00
abstract::CDC6, a replication licensing protein, is partially exported to the cytoplasm in human cells through phosphorylation by Cdk during S phase, but a significant proportion remains in the nucleus. We report here that human CDC6 physically interacts with ATR, a crucial checkpoint kinase, in a manner that is stimulated by p...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.058693
更新日期:2010-01-15 00:00:00
abstract::Wound healing crucially relies on the mechanical activity of fibroblasts responding to TGFβ1 and to forces transmitted across focal adhesions. Integrin-linked kinase (ILK) is a central adapter recruited to integrin β1 tails in focal adhesions mediating the communication between cells and extracellular matrix. Here, we...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.063024
更新日期:2010-11-15 00:00:00
abstract::Truncated Bid (tBid) releases cytochrome c from mitochondria by inducing Bak (and Bax) pore formation in the outer membrane. An important issue is whether a second tBid action, independent of Bak and Bax, is also required to enhance cytochrome c mobility in the intermembrane spaces. To investigate this, we developed a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.021303
更新日期:2008-03-01 00:00:00
abstract::The cylindrical cells of Schizosaccharomyces pombe grow in length by extension at the ends and not the middle. At the beginning of the cell cycle, growth is restricted to the 'old end', which existed in the previous cycle. Later on, the 'new end', formed from the septum, starts to grow at a point in the cycle that we ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00