Abstract:
:The role of basal epithelial cells in prostatic function, development and carcinogenesis is unknown. The ability of basal prostatic epithelial cells to acquire a luminal phenotype was explored in vitro using the NRP-152 rat dorsal-lateral prostate epithelial cell line as a model system. NRP-152, which was spontaneously immortalized and clonally derived, is an androgen-responsive and nontumorigenic cell line that has a basal cell phenotype under normal growth conditions. However, when placed in mitogen-deficient media, these cells undergo a dramatic morphological change to a luminal phenotype. Under these growth-restrictive conditions, immunocytochemical analysis shows that NRP-152 cells acquire the luminal markers Z0-1 (a tight-junction associated protein), occludin (integral tight-junction protein), and cytokeratin 18, and lose the basal markers cytokeratins 5 and 14. Total protein and mRNA levels of cytokeratins 8, 18, c-CAM 105 (the calcium-independent cell adhesion molecule) and Z0-1, as detected by western and/or northern blot analyses, respectively, are induced, while cytokeratin 5 and 15 are lost, and occludin is unchanged. Concomitant with this differentiation, expression of transforming growth factor-beta2 (TGF-beta2), TGF-beta3, and TGF-beta receptor type II (TbetaRII) is induced, while those of TGF-beta1 and TbetaRI remain essentially unchanged. Mitogens, such as insulin-like growth factor-I and dexamethasone inhibit luminal differentiation, while exogenous TGF-beta induces such differentiation. These data together with TGF-beta neutralization experiments using pan-specific antibody implicate an important role for autocrine TGF-beta in the induction of the luminal differentiation.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Danielpour Dsubject
Has Abstractpub_date
1999-01-01 00:00:00pages
169-79eissn
0021-9533issn
1477-9137journal_volume
112 ( Pt 2)pub_type
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00352
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pub_type: 杂志文章,评审
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.167932
更新日期:2016-03-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1991-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-04-01 00:00:00
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
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