当前位置： SCI文献检索 > JOURNAL OF CELL SCIENCE期刊下所有文献 > Factor inhibiting HIF-1 (FIH-1) modulates protein interactions of apoptosis-stimulating p53 binding protein 2 (ASPP2).

Factor inhibiting HIF-1 (FIH-1) modulates protein interactions of apoptosis-stimulating p53 binding protein 2 (ASPP2).

Abstract：

:The asparaginyl hydroxylase factor inhibiting HIF-1 (FIH-1) is an important suppressor of hypoxia-inducible factor (HIF) activity. In addition to HIF-α, FIH-1 was previously shown to hydroxylate other substrates within a highly conserved protein interaction domain, termed the ankyrin repeat domain (ARD). However, to date, the biological role of FIH-1-dependent ARD hydroxylation could not be clarified for any ARD-containing substrate. The apoptosis-stimulating p53-binding protein (ASPP) family members were initially identified as highly conserved regulators of the tumour suppressor p53. In addition, ASPP2 was shown to be important for the regulation of cell polarity through interaction with partitioning defective 3 homolog (Par-3). Using mass spectrometry we identified ASPP2 as a new substrate of FIH-1 but inhibitory ASPP (iASPP) was not hydroxylated. We demonstrated that ASPP2 asparagine 986 (N986) is a single hydroxylation site located within the ARD. ASPP2 protein levels and stability were not affected by depletion or inhibition of FIH-1. However, FIH-1 depletion did lead to impaired binding of Par-3 to ASPP2 while the interaction between ASPP2 and p53, apoptosis and proliferation of the cancer cells were not affected. Depletion of FIH-1 and incubation with the hydroxylase inhibitor dimethyloxalylglycine (DMOG) resulted in relocation of ASPP2 from cell-cell contacts to the cytosol. Our data thus demonstrate that protein interactions of ARD-containing substrates can be modified by FIH-1-dependent hydroxylation. The large cellular pool of ARD-containing proteins suggests that FIH-1 can affect a broad range of cellular functions and signalling pathways under certain conditions, for example, in response to severe hypoxia.

journal_name

J Cell Sci

journal_title

Journal of cell science

authors

Janke K,Brockmeier U,Kuhlmann K,Eisenacher M,Nolde J,Meyer HE,Mairbäurl H,Metzen E

doi

10.1242/jcs.117564

subject

Has Abstract

pub_date

2013-06-15 00:00:00

pages

2629-40

issue

Pt 12

eissn

0021-9533

issn

1477-9137

pii

jcs.117564

journal_volume

126

pub_type

杂志文章

相关文献

JOURNAL OF CELL SCIENCE文献大全
  • A PIM-CHK1 signaling pathway regulates PLK1 phosphorylation and function during mitosis.

    abstract：:Although the kinase CHK1 is a key player in the DNA damage response (DDR), several studies have recently provided evidence of DDR-independent roles of CHK1, in particular following phosphorylation of its S280 residue. Here, we demonstrate that CHK1 S280 phosphorylation is cell cycle-dependent and peaks during mitosis....

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.213116

    authors： Adam K,Cartel M,Lambert M,David L,Yuan L,Besson A,Mayeux P,Manenti S,Didier C

    更新日期：2018-08-10 00:00:00

  • Controlling DNA replication origins in response to DNA damage - inhibit globally, activate locally.

    abstract：:DNA replication in eukaryotic cells initiates from multiple replication origins that are distributed throughout the genome. Coordinating the usage of these origins is crucial to ensure complete and timely replication of the entire genome precisely once in each cell cycle. Replication origins fire according to a cell-t...

    journal_title：Journal of cell science

    pub_type： 杂志文章,评审

    doi：10.1242/jcs.096701

    authors： Yekezare M,Gómez-González B,Diffley JF

    更新日期：2013-03-15 00:00:00

  • FGF2-induced Ras-MAPK signalling maintains lymphatic endothelial cell identity by upregulating endothelial-cell-specific gene expression and suppressing TGFβ signalling through Smad2.

    abstract：:The lymphatic endothelial cell (LEC) fate decision program during development has been described. However, the mechanism underlying the maintenance of differentiated LEC identity remains largely unknown. Here, we show that fibroblast growth factor 2 (FGF2) plays a fundamental role in maintaining a differentiated LEC t...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.137836

    authors： Ichise T,Yoshida N,Ichise H

    更新日期：2014-02-15 00:00:00

  • Structural basis for the nuclear import of the human androgen receptor.

    abstract：:Ligand-dependent nuclear import is crucial for the function of the androgen receptor (AR) in both health and disease. The unliganded AR is retained in the cytoplasm but, on binding 5alpha-dihydrotestosterone, it translocates into the nucleus and alters transcription of its target genes. Nuclear import of AR is mediate...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.022103

    authors： Cutress ML,Whitaker HC,Mills IG,Stewart M,Neal DE

    更新日期：2008-04-01 00:00:00

  • Lack of CD2AP disrupts Glut4 trafficking and attenuates glucose uptake in podocytes.

    abstract：:The adapter protein CD2-associated protein (CD2AP) functions in various signaling and vesicle trafficking pathways, including endosomal sorting and/or trafficking and degradation pathways. Here, we investigated the role of CD2AP in insulin-dependent glucose transporter 4 (Glut4, also known as SLC2A4) trafficking and g...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.175075

    authors： Tolvanen TA,Dash SN,Polianskyte-Prause Z,Dumont V,Lehtonen S

    更新日期：2015-12-15 00:00:00

  • Cooperative interactions of LPPR family members in membrane localization and alteration of cellular morphology.

    abstract：:The lipid phosphate phosphatase-related proteins (LPPRs), also known as plasticity-related genes (PRGs), are classified as a new brain-enriched subclass of the lipid phosphate phosphatase (LPP) superfamily. They induce membrane protrusions, neurite outgrowth or dendritic spine formation in cell lines and primary neuro...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.169789

    authors： Yu P,Agbaegbu C,Malide DA,Wu X,Katagiri Y,Hammer JA,Geller HM

    更新日期：2015-09-01 00:00:00

  • Protein synthesizing units in presynaptic and postsynaptic domains of squid neurons.

    abstract：:Putative protein synthesizing domains, called plaques, are characterized in the squid giant synapse and axon and in terminals of squid photoreceptor neurons. Plaques are oval-shaped formations of about 1 microm in size, which (1) generate signals that have spectroscopic electron energy loss characteristics of ribosome...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Martin R,Vaida B,Bleher R,Crispino M,Giuditta A

    更新日期：1998-11-01 00:00:00

  • Controlled induction of focal adhesion disassembly and migration in primary fibroblasts.

    abstract：:Fibroblast migration is an integral component of biological processes such as wound healing and embryogenesis. Previous experiments examining fibroblast locomotion from tissue explants have shown that migrating fibroblasts lack, or contain only transient, focal adhesions (focal contacts). Focal adhesions are specializ...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Dunlevy JR,Couchman JR

    更新日期：1993-06-01 00:00:00

  • Intermediate filament interactions can be altered by HSP27 and alphaB-crystallin.

    abstract：:HSP27 and alphaB-crystallin are both members of the small heat shock protein family. alphaB-crystalllin has been proposed to modulate intermediate filaments and recently a mutation in alphaB-crystallin has been identified as the genetic basis of desmin related myopathy. This disease is characterised in its pathology b...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Perng MD,Cairns L,van den IJssel P,Prescott A,Hutcheson AM,Quinlan RA

    更新日期：1999-07-01 00:00:00

  • Myosin VI and its cargo adaptors - linking endocytosis and autophagy.

    abstract：:The coordinated trafficking and tethering of membrane cargo within cells relies on the function of distinct cytoskeletal motors that are targeted to specific subcellular compartments through interactions with protein adaptors and phospholipids. The unique actin motor myosin VI functions at distinct steps during clathr...

    journal_title：Journal of cell science

    pub_type： 杂志文章,评审

    doi：10.1242/jcs.095554

    authors： Tumbarello DA,Kendrick-Jones J,Buss F

    更新日期：2013-06-15 00:00:00

  • The ADAMTS12 metalloproteinase exhibits anti-tumorigenic properties through modulation of the Ras-dependent ERK signalling pathway.

    abstract：:Members of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family of proteolytic enzymes are implicated in a variety of physiological processes, such as collagen maturation, organogenesis, angiogenesis, reproduction and inflammation. Moreover, deficiency or overexpression of certain ADAMTS ...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.005751

    authors： Llamazares M,Obaya AJ,Moncada-Pazos A,Heljasvaara R,Espada J,López-Otín C,Cal S

    更新日期：2007-10-15 00:00:00

  • Double-replica topography of disk membrane structures in the carp (Cyprinus carpio).

    abstract：:By an improved freeze-fracture device, a number of double replicas were obtained from the rod disk membranes of the carp retina for investigation of complementary, continuing, and transitional relationships between fracture faces. Some separate particles found along the smooth edge bordering on the adjacent interdisk ...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Tonosaki A,Washioka H,Yamasaki M,Mizoguchi J

    更新日期：1980-04-01 00:00:00

  • Functional contribution of Pds5 to cohesin-mediated cohesion in human cells and Xenopus egg extracts.

    abstract：:Sister chromatid cohesion is essential for proper segregation of the genome in mitosis and meiosis. Central to this process is cohesin, a multi-protein complex conserved from yeast to human. Previous genetic studies in fungi have identified Pds5/BimD/Spo76 as an additional factor implicated in cohesion. Here we descri...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.02355

    authors： Losada A,Yokochi T,Hirano T

    更新日期：2005-05-15 00:00:00

  • The CCM1-CCM2 complex controls complementary functions of ROCK1 and ROCK2 that are required for endothelial integrity.

    abstract：:Endothelial integrity relies on a mechanical crosstalk between intercellular and cell-matrix interactions. This crosstalk is compromised in hemorrhagic vascular lesions of patients carrying loss-of-function mutations in cerebral cavernous malformation (CCM) genes. RhoA/ROCK-dependent cytoskeletal remodeling is central...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.216093

    authors： Lisowska J,Rödel CJ,Manet S,Miroshnikova YA,Boyault C,Planus E,De Mets R,Lee HH,Destaing O,Mertani H,Boulday G,Tournier-Lasserve E,Balland M,Abdelilah-Seyfried S,Albiges-Rizo C,Faurobert E

    更新日期：2018-08-13 00:00:00

  • Collagen V is a dominant regulator of collagen fibrillogenesis: dysfunctional regulation of structure and function in a corneal-stroma-specific Col5a1-null mouse model.

    abstract：:Collagen V is a regulatory fibril-forming collagen that forms heterotypic fibrils with collagen I. Deletion of collagen V in the mouse is associated with a lack of fibril assembly in the embryonic mesenchyme, with a resultant lethal phenotype. The current work elucidates the regulatory roles of collagen V during devel...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.091363

    authors： Sun M,Chen S,Adams SM,Florer JB,Liu H,Kao WW,Wenstrup RJ,Birk DE

    更新日期：2011-12-01 00:00:00

  • Micro-environmental control of cell migration--myosin IIA is required for efficient migration in fibrillar environments through control of cell adhesion dynamics.

    abstract：:Recent evidence suggests that organization of the extracellular matrix (ECM) into aligned fibrils or fibril-like ECM topographies promotes rapid migration in fibroblasts. However, the mechanisms of cell migration that are altered by these changes in micro-environmental topography remain unknown. Here, using 1D fibrill...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.098806

    authors： Doyle AD,Kutys ML,Conti MA,Matsumoto K,Adelstein RS,Yamada KM

    更新日期：2012-05-01 00:00:00

  • The genetic depletion or the triptolide inhibition of TFIIH in p53-deficient cells induces a JNK-dependent cell death in Drosophila.

    abstract：:Transcription factor IIH (TFIIH) participates in transcription, nucleotide excision repair and the control of the cell cycle. In the present study, we demonstrate that the Dmp52 subunit of TFIIH in Drosophila physically interacts with the fly p53 homologue, Dp53. The depletion of Dmp52 in the wing disc generates chrom...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.122721

    authors： Villicaña C,Cruz G,Zurita M

    更新日期：2013-06-01 00:00:00

  • The C-terminal domain LLKIL motif of CXCR2 is required for ligand-mediated polarization of early signals during chemotaxis.

    abstract：:HEK293 cells expressing wild-type CXCR2 recruit PH-Akt-GFP to the leading edge of the cell in response to chemokine. However, in cells expressing mutant CXCR2 defective in AP-2 and HIP binding, i.e. with a mutation in the LLKIL motif, PH-Akt-GFP does not localize to the leading edge in response to ligand. Inhibition o...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.01398

    authors： Sai J,Fan GH,Wang D,Richmond A

    更新日期：2004-11-01 00:00:00

  • CD98 at the crossroads of adaptive immunity and cancer.

    abstract：:Adaptive immunity, a vertebrate specialization, adds memory and exquisite specificity to the basic innate immune responses present in invertebrates while conserving metabolic resources. In adaptive immunity, antigenic challenge requires extremely rapid proliferation of rare antigen-specific lymphocytes to produce larg...

    journal_title：Journal of cell science

    pub_type： 杂志文章,评审

    doi：10.1242/jcs.096040

    authors： Cantor JM,Ginsberg MH

    更新日期：2012-03-15 00:00:00

  • Meiotic maturation of mouse oocytes in vitro: association of newly synthesized proteins with condensing chromosomes.

    abstract：:The nature, intracellular distribution, and role of proteins synthesized during meiotic maturation of mouse oocytes in vitro have been examined. Proteins synthesized during the initial stages of maturation are concentrated within the nucleus (germinal vesicle) and become intimately associated with the condensing chrom...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Wassarman PM,Letourneau GE

    更新日期：1976-05-01 00:00:00

  • Characterization of a novel nuclear envelope protein restricted to certain cell types.

    abstract：:The monoclonal antibody AGF2.3 identifies a nuclear envelope protein that is restricted to certain cell types. In particular, this antigen shows a reduced level of expression during haemopoietic cell maturation. In this study, we have examined the relationship of this protein to known nuclear envelope proteins that ha...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Lord JM,Thick JA,Bunce CM,Taylor AM,Gallimore PH,Mills D,Brown G

    更新日期：1988-06-01 00:00:00

  • Ciliopathy proteins establish a bipartite signaling compartment in a C. elegans thermosensory neuron.

    abstract：:How signaling domains form is an important, yet largely unexplored question. Here, we show that ciliary proteins help establish two contiguous, yet distinct cyclic GMP (cGMP) signaling compartments in Caenorhabditis elegans thermosensory AFD neurons. One compartment, a bona fide cilium, is delineated by proteins assoc...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.157610

    authors： Nguyen PA,Liou W,Hall DH,Leroux MR

    更新日期：2014-12-15 00:00:00

  • Live cell imaging and electron microscopy reveal dynamic processes of BAF-directed nuclear envelope assembly.

    abstract：:Assembly of the nuclear envelope (NE) in telophase is essential for higher eukaryotic cells to re-establish a functional nucleus. Time-lapse, FRAP and FRET analyses in human cells showed that barrier-to-autointegration factor (BAF), a DNA-binding protein, assembled first at the distinct ;core' region of the telophase ...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.033597

    authors： Haraguchi T,Kojidani T,Koujin T,Shimi T,Osakada H,Mori C,Yamamoto A,Hiraoka Y

    更新日期：2008-08-01 00:00:00

  • TrkA mediates retrograde semaphorin 3A signaling through plexin A4 to regulate dendritic branching.

    abstract：:Semaphorin 3A (Sema3A), a secretory semaphorin, exerts various biological actions through a complex between neuropilin-1 and plexin-As (PlexAs). Sema3A induces retrograde signaling, which is involved in regulating dendritic localization of GluA2 (also known as GRIA2), an AMPA receptor subunit. Here, we investigated a ...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.184580

    authors： Yamashita N,Yamane M,Suto F,Goshima Y

    更新日期：2016-05-01 00:00:00

  • Drebrin preserves endothelial integrity by stabilizing nectin at adherens junctions.

    abstract：:Regulation of cell-cell contacts is essential for integrity of the vascular endothelium. Here, a critical role of the F-actin-binding protein drebrin in maintaining endothelial integrity is revealed under conditions mimicking vascular flow. Drebrin knockdown leads to weakening of cell-cell contacts, characterized by l...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.129437

    authors： Rehm K,Panzer L,van Vliet V,Genot E,Linder S

    更新日期：2013-08-15 00:00:00

  • The Drosophila forked protein induces the formation of actin fiber bundles in vertebrate cells.

    abstract：:The forked protein is an actin binding protein involved in the formation of large actin fiber bundles in developing Drosophila bristles. These are the largest example of a type of actin bundle characterized by parallel, hexagonally packed actin fibers, also found in intestinal microvilli, kidney proximal tubule microv...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Grieshaber S,Petersen NS

    更新日期：1999-07-01 00:00:00

  • Low-intensity pulsed ultrasound promotes cell motility through vinculin-controlled Rac1 GTPase activity.

    abstract：:Low-intensity pulsed ultrasound (LIPUS) is a therapy used clinically to promote healing. Using live-cell imaging we show that LIPUS stimulation, acting through integrin-mediated cell-matrix adhesions, rapidly induces Rac1 activation associated with dramatic actin cytoskeleton rearrangements. Our study demonstrates tha...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.192781

    authors： Atherton P,Lausecker F,Harrison A,Ballestrem C

    更新日期：2017-07-15 00:00:00

  • abnormal chromatin (abc), a maternal-effect locus in Drosophila melanogaster.

    abstract：:Mutations in the maternal-effect gene abnormal chromatin (abc) in Drosophila melanogaster result in a variety of defects involving nuclear replication/division. Three recessive alleles of this gene, which maps near 51F on chromosome 2, all result in female sterility. They cause slower embryonic development that is usu...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Vessey KB,Ludwiczak RL,Briot AS,Underwood EM

    更新日期：1991-02-01 00:00:00

  • Tumor necrosis factor-alpha (TNFalpha) regulates the epithelial barrier in the human intestinal cell line HT-29/B6.

    abstract：:Cytokines are supposed to be mediators in diarrhoeal diseases. The aim of this study is to characterize the effect of tumor necrosis factor-alpha (TNFalpha) on epithelial barrier function in the colonic epithelial cell line HT-29/B6. Active ion transport and barrier function were measured as short-circuit current and ...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Schmitz H,Fromm M,Bentzel CJ,Scholz P,Detjen K,Mankertz J,Bode H,Epple HJ,Riecken EO,Schulzke JD

    更新日期：1999-01-01 00:00:00

  • Targeting of the myosin-I myr 3 to intercellular adherens type junctions induced by dominant active Cdc42 in HeLa cells.

    abstract：:Myr 3, a member of the myosin-I family from rat, is shown in this study to be localized at adherens-type intercellular junctions in epithelial and nonepithelial tissues. Formation of intercellular junctions and the accompanying recruitment of myr 3 to these junctions involves signaling by the Rho subfamily of small GT...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Stöffler HE,Honnert U,Bauer CA,Höfer D,Schwarz H,Müller RT,Drenckhahn D,Bähler M

    更新日期：1998-09-01 00:00:00