Abstract：
:The lymphatic endothelial cell (LEC) fate decision program during development has been described. However, the mechanism underlying the maintenance of differentiated LEC identity remains largely unknown. Here, we show that fibroblast growth factor 2 (FGF2) plays a fundamental role in maintaining a differentiated LEC trait. In addition to demonstrating the appearance of LECs expressing α-smooth muscle actin in mouse lymphedematous skin in vivo, we found that mouse immortalised LECs lose their characteristics and undergo endothelial-to-mesenchymal transition (EndMT) when cultured in FGF2-depleted medium. FGF2 depletion acted synergistically with transforming growth factor (TGF) β to induce EndMT. We also found that H-Ras-overexpressing LECs were resistant to EndMT. Activation of H-Ras not only upregulated FGF2-induced activation of the Erk mitogen activated protein kinases (MAPK3 and MAPK1), but also suppressed TGFβ-induced activation of Smad2 by modulating Smad2 phosphorylation by MAPKs. These results suggest that FGF2 regulates LEC-specific gene expression and suppresses TGFβ signalling in LECs through Smad2 in a Ras-MAPK-dependent manner. Taken together, our findings provide a new insight into the FGF2-Ras-MAPK-dependent mechanism that maintains and modulates the LEC trait.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Ichise T,Yoshida N,Ichise Hdoi
10.1242/jcs.137836subject
Has Abstractpub_date
2014-02-15 00:00:00pages
845-57issue
Pt 4eissn
0021-9533issn
1477-9137pii
jcs.137836journal_volume
127pub_type
杂志文章abstract：:Serotonin receptors 5-HT(1A) and 5-HT(7) are highly coexpressed in brain regions implicated in depression. However, their functional interaction has not been established. In the present study we show that 5-HT(1A) and 5-HT(7) receptors form heterodimers both in vitro and in vivo. Foerster resonance energy transfer-bas...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.101337
更新日期：2012-05-15 00:00:00
abstract：:We have isolated a fourth myosin gene (MYO4) in yeast (Saccharomyces cerevisiae). MYO4 encodes a approximately 170 kDa (1471 amino acid) class V myosin, using the classification devised by Cheney et al. (1993a; Cell Motil. Cytoskel. 24, 215-223); the motor domain is followed by a neck region containing six putative ca...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:During neuronal development, netrin and its receptors UNC5 and DCC (deleted in colorectal cancer) guide axonal growth cones in navigating to their targets. Netrin also plays important roles in the regulation of cell migration, tissue morphogenesis and tumor growth. Here, we show that netrin induces UNC5 tyrosine phosp...
journal_title：Journal of cell science
pub_type： 杂志文章,收录出版
doi：10.1242/jcs.02697
更新日期：2006-01-01 00:00:00
abstract：:Rickettsia conorii, the causative agent of Mediterranean spotted fever, is able to attach to and invade a variety of cell types both in vitro and in vivo. Although previous studies show that entry of R. conorii into non-phagocytic cells relies on actin polymerization, little else is known about the molecular details g...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01382
更新日期：2004-10-01 00:00:00
abstract：:PML nuclear bodies (NBs) are involved in the regulation of key nuclear pathways but their biochemical function in nuclear metabolism is unknown. In this study PML NB assembly dynamics were assessed by live cell imaging and mathematic modeling of its major component parts. We show that all six nuclear PML isoforms exhi...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.031922
更新日期：2008-08-15 00:00:00
abstract：:Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly regulated by its post-translational modifications. AnxA2 and its Tyr23-phosphorylated form (pTyr23AnxA2) are involved in malignant cell transformation, metastasis and angiogenesis. Here, we show...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.173195
更新日期：2016-01-15 00:00:00
abstract：:The monoclonal antibody AGF2.3 identifies a nuclear envelope protein that is restricted to certain cell types. In particular, this antigen shows a reduced level of expression during haemopoietic cell maturation. In this study, we have examined the relationship of this protein to known nuclear envelope proteins that ha...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:In the cytoplasm of chlorenchyma cells in primary Origanum dictamnus leaves, rectangular prism-like crystals occur, which, as shown by pepsin digestion, are proteinaceous. In sections they usually show either a square lattice or striations running parallel to the short or long axis. In both cases the interspacings are...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:Addition of lysophosphatidic acid (LPA) to serum-deprived N1E-115 neuronal cells results in rapid f-actin assembly accompanied by neurite retraction and rounding of the cell body due to contraction of the cortical actin cytoskeleton. LPA action is mimicked by activated RhoA, while it is blocked by dominant-negative Rh...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1997-10-01 00:00:00
abstract：:The Tec kinases represent the second largest family of mammalian non-receptor tyrosine kinases and are distinguished by the presence of distinct proline-rich regions and pleckstrin homology domains that are required for proper regulation and activation. Best studied in lymphocyte and mast cells, these kinases are crit...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：
更新日期：2002-08-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:A correlation between intranuclear distribution of large tumor antigen (T-ag) and proliferative activity in Simian virus 40 (SV40)-transformed cells has been established. Nuclear T-ag was cytochemically detected by the immunoperoxidase reaction. Cell populations with a high growth rate displayed a heterogeneous patter...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
abstract：:The contractile vacuole of the chloromonadophycean alga Vacuolaria virescens is a permanent structure that possesses a specialized membrane: subunits of this membrane have a diameter of 21-24 nm and in places are arranged in a regular hexagonal pattern. The lateral walls of these subunits form regularly spaced bristle...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-06-01 00:00:00
abstract：:We have shown previously, by fluorescent antibody staining, that desmosomal antigens are widely distributed in the tissues of vertebrate animals. Furthermore, we have demonstrated mutual desmosome formation between cells derived from man, cow, dog, chicken and frog. In this paper we have studied the components of desm...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:Transcriptional activation of the major histocompatibility complex (MHC) by IFNgamma is a key step in cell-mediated immunity. At an early stage of IFNgamma induction, chromatin carrying the entire MHC locus loops out from the chromosome 6 territory. We show here that JAK/STAT signalling triggers this higher-order chro...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.012328
更新日期：2007-09-15 00:00:00
abstract：:Survivin, a human inhibitor of apoptosis protein (IAP), plays an important role in both cell cycle regulation and inhibition of apoptosis. Survivin is expressed in cells during the G(2)/M phase of the cell cycle, followed by rapid decline of both mRNA and protein levels at the G(1) phase. It has been suggested that ce...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：:The dynamics of cell adhesion sites control cell morphology and motility. Adhesion-site turnover is thought to depend on the local availability of the acidic phospholipid phosphatidylinositol-4,5-bisphosphate (PIP(2)). PIP(2) can bind to many cell adhesion proteins such as vinculin and talin, but the consequences of t...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01734
更新日期：2005-04-01 00:00:00
abstract：:Expression of actin was examined in a cultured rat embryonic cell line 3Y1 and transformed cell lines that originated from 3Y1. An alpha-actin in addition to cytoplasmic beta- and gamma-actins was detected in 3Y1 by two-dimensional gel electrophoresis. This alpha-actin was hardly detected at all in the transformants i...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:Aggregation capacity of chicken embryo chondrocytes decreases when transformed by Rous sarcoma viruses. Cell-to-cell aggregation capacity of chondrocytes infected with a T class temperature-sensitive mutant (tsNY68) (with the temperature-sensitive lesion at the src gene) of Rous sarcoma virus is dependent upon the tem...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1980-06-01 00:00:00
abstract：:The forked protein is an actin binding protein involved in the formation of large actin fiber bundles in developing Drosophila bristles. These are the largest example of a type of actin bundle characterized by parallel, hexagonally packed actin fibers, also found in intestinal microvilli, kidney proximal tubule microv...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:During the interphase to metaphase transition, microtubules are destabilized by a cdc2 kinase-dependent phosphorylation event. This destabilization is due to a dramatic increase in the rate at which each growing microtubule starts to shrink (catastrophe rate). In principle, this could be brought about by lowering the ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-08-01 00:00:00
abstract：:Neurite outgrowth is known as a slow (days) process occurring in nerve cells and neurons during neurotrophin treatment and upon transfer to culture, respectively. Using Y27632, a drug that induces activation of Rac1, a downstream step of the neurotrophin signaling cascade, we have identified a new form of outgrowth, w...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.059634
更新日期：2010-01-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
abstract：:Cell fusion plays a well-recognized, physiological role during development. Bone-marrow-derived hematopoietic cells have been shown to fuse with non-hematopoietic cells in a wide variety of tissues. Some organs appear to resolve the changes in ploidy status, generating functional and mitotically-competent events. Howe...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.100123
更新日期：2012-06-15 00:00:00
abstract：:The constitutive recirculation of lymphocytes between the widely distributed organs of the immune system is essential for host defence. We have developed an in vitro model of lymphocyte migration from the blood into lymph nodes which employs primary cultures of high endothelial cells (HEC). HEC-adherent lymphocytes ad...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00403
更新日期：2003-05-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1980-12-01 00:00:00
