Abstract:
:Aggregation capacity of chicken embryo chondrocytes decreases when transformed by Rous sarcoma viruses. Cell-to-cell aggregation capacity of chondrocytes infected with a T class temperature-sensitive mutant (tsNY68) (with the temperature-sensitive lesion at the src gene) of Rous sarcoma virus is dependent upon the temperature at which these cells are grown. When grown at the permissive temperature (36 degrees C), where the transforming gene is expressed, aggregation capacity was lower than normal while infected cells grown at the non-permissive temperature (41.5 degrees C) had similar capacity to aggregate to that of normal chondrocytes. However, after a prolonged period of culture (10 days), chondrocytes transformed by wild type SR-RSV regained the normal level of aggregation capacity. Cells transformed by tsNY68 and incubated at the permissive temperature for 10 days also regained the normal level of aggregation capacity. It appears therefore that RSV-transformed chondrocytes first become less adhesive but during long-term cultivation they regain their property to aggregate. The decrease of aggregation capacity due to T class mutants of RSV at 36 degrees C is dependent on constant maintenance of protein synthesis because addition of cycloheximide restored the aggregation capacity even at the permissive temperature.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Tanaka A,Kaji Asubject
Has Abstractpub_date
1980-06-01 00:00:00pages
407-17eissn
0021-9533issn
1477-9137journal_volume
43pub_type
杂志文章abstract::As previously described, a cell surface-associated adhesive factor (AF) was separated from differentiated rat ascites hepatoma AH136B cells (forming cell islands in vivo) and highly purified by chromatography. AF synthesis by the cells was investigated by means of ultrastructural immunoperoxidase cytochemistry. Its sy...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1984-10-01 00:00:00
abstract::Cilia and flagella are conserved eukaryotic organelles important for motility and sensory. The RanGTPase, best known for nucleocytoplasmic transport functions, may also play a role in protein trafficking into the specialized flagellar/ciliary compartments, although the regulatory mechanisms controlling Ran activity at...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.148015
更新日期:2014-11-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.145862
更新日期:2014-03-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.181230
更新日期:2016-02-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.076976
更新日期:2011-03-15 00:00:00
abstract::The cell types expressing cot proto-oncogene mRNA were identified by in situ hybridization (ISH) histochemistry. Among a variety of adult mouse tissues examined, four types of glandular cells expressing cot gene were identified: (1) granular duct cells in the submandibular and sublingual glands; (2) serous cells in th...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1977-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
abstract::Membrane contact sites (MCSs), where the membranes of two organelles are closely apposed, are regions where small molecules such as lipids or calcium are exchanged between organelles. We have identified a conserved membrane-binding domain found exclusively in proteins at MCSs in Saccharomyces cerevisiae. The synaptota...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.085118
更新日期:2012-01-01 00:00:00
abstract::Rap1 is a Ras-like small GTPase that is activated by many extracellular stimuli and strongly implicated in the control of integrin-mediated cell adhesion. Recent evidence indicates that Rap1 also plays a key role in formation of cadherin-based cell-cell junctions. Indeed, inhibition of Rap1 generates immature adherens...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.03306
更新日期:2007-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-06-01 00:00:00
abstract::Previously, we demonstrated that proteolytic processing within the globular domain of the alpha3 subunit of laminin-5 (LN5) converts LN5 from a cell motility-inducing factor to a protein complex that can trigger the formation of hemidesmosomes, certain cell-matrix attachment sites found in epithelial cells. We have pr...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.136176
更新日期:2013-12-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.051912
更新日期:2009-07-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.220327
更新日期:2018-09-03 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.250738
更新日期:2020-12-21 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03086
更新日期:2006-09-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.084152
更新日期:2012-06-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.036152
更新日期:2008-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02720
更新日期:2006-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.224311
更新日期:2019-04-30 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1981-12-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1984-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2019-03-07 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
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