Abstract:
:During the interphase to metaphase transition, microtubules are destabilized by a cdc2 kinase-dependent phosphorylation event. This destabilization is due to a dramatic increase in the rate at which each growing microtubule starts to shrink (catastrophe rate). In principle, this could be brought about by lowering the affinity of stabilizing MAPs for the microtubule wall, by activating a factor that would actively increase the catastrophe rate or by an alteration of both parameters. Here we examine the stabilizing effect of bovine brain MAP2 on microtubules assembled in interphase Xenopus egg extracts. We show that this MAP strongly stabilizes microtubules assembled in the extracts against nocodazole-induced depolymerization. However, it does not protect them from the cdc2 kinase-induced shortening and destabilization. Moreover, the steady-state length of centrosome-nucleated microtubules in cdc2-treated extracts containing MAP2 is similar to that found in extracts lacking exogenous MAP2. We also show that although exogenous MAP2 is phosphorylated by cdc2 kinase in the extract, this is not the cause of microtubule destabilization. These results indicate that increased microtubule dynamics during mitosis is due to the activation of a factor that can function independently of the presence of active, stabilizing factors.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Faruki S,Dorée M,Karsenti Ekeywords:
subject
Has Abstractpub_date
1992-01-01 00:00:00pages
69-78eissn
0021-9533issn
1477-9137journal_volume
101 ( Pt 1)pub_type
杂志文章abstract::Membrane-type matrix metalloproteinase 1 (MT1-MMP) is a membrane-anchored matrix metalloproteinase (MMP) that is frequently associated with processes involving tissue remodelling and cell migration. We have examined MT1-MMP expression and subcellular distribution as a function of MCF10A mammary epithelial cell migrati...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::Casein kinase 1 (CK1) plays central roles in various signal transduction pathways and performs many cellular activities. For many years CK1 was thought to act independently of modulatory subunits and in a constitutive manner. Recently, DEAD box RNA helicases, in particular DEAD box RNA helicase 3 X-linked (DDX3X), wer...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.207316
更新日期:2018-01-04 00:00:00
abstract::Freeze-fracture cytochemistry with digitonin has been used to examine the cholesterol content of the plasma membrane of uterine epithelial cells during the early stages of pregnancy in the rat. Lesions caused by digitonin complexing with cholesterol were seen on both lateral and apical portions of the membrane but tig...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-10-01 00:00:00
abstract::We uncovered a crucial role for the Aurora kinase A (AURKA)-Twist1 axis in promoting epithelial-to-mesenchymal transition (EMT) and chemoresistance in pancreatic cancer. Twist1 is the first EMT-specific target of AURKA that was identified using an innovative screen. AURKA phosphorylates Twist1 at three sites, which re...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.196790
更新日期:2017-03-15 00:00:00
abstract::p63 (also known as TP63) is a transcription factor of the p53 family, along with p73. Multiple isoforms of p63 have been discovered and these have diverse functions encompassing a wide array of cell biology. p63 isoforms are implicated in lineage specification, proliferative potential, differentiation, cell death and ...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.228015
更新日期:2020-09-11 00:00:00
abstract::p97 (CDC-48 in Caenorhabditis elegans) is a ubiquitin-selective AAA (ATPases associated with diverse cellular activities) chaperone and its key function is to disassemble protein complexes. p97 functions in diverse cellular processes including endoplasmic reticulum (ER)-associated degradation, membrane fusion, and mei...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.052415
更新日期:2009-10-15 00:00:00
abstract::It is generally accepted that global translation varies during the cell cycle and is low during mitosis. However, addressing this issue is challenging because it involves cell synchronization, which evokes stress responses that, in turn, affect translation rates. Here, we have used two approaches to measure global tra...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.220327
更新日期:2018-09-03 00:00:00
abstract::Nearly all intermediate filament (IF) proteins share two sequence motifs located at the N- and the C-terminal ends of their helical rod domain ('coil 1a' and 'coil 2b', respectively). To examine the structural role of the coil 2b motif, we have performed in vitro assembly studies and in vivo microinjection experiments...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract::Cell chemotaxis, such as migration of fibroblasts towards growth factors during development and wound healing, requires precise spatial coordination of signalling events. Phosphoinositides and signalling enzymes involved in their generation and hydrolysis have been implicated in regulation of chemotaxis; however, the ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.110007
更新日期:2012-12-01 00:00:00
abstract::Dynamic assembly and remodeling of actin is critical for many cellular processes during development and stress adaptation. In filamentous fungi and budding yeast, actin cables align in a polarized manner along the mother-to-daughter cell axis, and are essential for the establishment and maintenance of polarity; moreov...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.247916
更新日期:2021-01-08 00:00:00
abstract::The non-alpha-helical N-terminal domain of intermediate filament proteins plays a key role in filament assembly. Previous studies have identified a nonapeptide motif, SSYRRIFGG, in the non-alpha-helical N-terminal domain of vimentin that is required for assembly. This motif is also found in desmin, peripherin and the ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-07-01 00:00:00
abstract::Dynamin-related GTPase proteins (DRPs) are main players in membrane remodelling. Conserved DRPs called mitofusins (Mfn1/Mfn2/Fzo1) mediate the fusion of mitochondrial outer membranes (OM). OM fusion depends on self-assembly and GTPase activity of mitofusins as well as on two other proteins, Ugo1 and Mdm30. Here, we de...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.073080
更新日期:2011-04-01 00:00:00
abstract::Homophilic binding of E-cadherins through their ectodomains is fundamental to epithelial cell-cell adhesion. Despite this, E-cadherin ectodomains have evolved differently in the vertebrate and insect lineages. Of the five rod-like, tandemly aligned extracellular cadherin domains of vertebrate E-cadherin, the tip extra...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.189258
更新日期:2016-09-01 00:00:00
abstract::We have identified and characterized two proteins in rat brain that bind to the neuron-specific tropomyosin isoform, TMBr3. The two proteins were identified by blot overlay assay, in which the proteins immobilized on the membrane were probed by epitope-tagged TMBr3, followed by detection with anti-epitope antibody. We...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract::The surface coat of procyclic forms of Trypanosoma brucei consists of related, internally repetitive glycoproteins known as EP and GPEET procyclins. Previously we showed that the extracellular domain of GPEET is phosphorylated. We now show that phosphorylation of this glycosylphosphatidylinositol-anchored surface prot...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::Members of the ezrin-radixin-moesin (ERM) family of proteins are involved in multiple aspects of cell migration by acting both as crosslinkers between the membrane, receptors and the actin cytoskeleton, and as regulators of signalling molecules that are implicated in cell adhesion, cell polarity and migration. Increas...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.133108
更新日期:2014-01-15 00:00:00
abstract::The transmembrane inner nuclear membrane (INM) protein Samp1 is required for anchoring centrosomes near the nuclei. Using high-resolution fluorescence microscopy we show that Samp1 is distributed in a distinct and characteristic pattern in the nuclear envelope (NE), where it partially colocalizes with the LINC complex...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.078923
更新日期:2011-06-15 00:00:00
abstract::The contractile vacuole of the chloromonadophycean alga Vacuolaria virescens is a permanent structure that possesses a specialized membrane: subunits of this membrane have a diameter of 21-24 nm and in places are arranged in a regular hexagonal pattern. The lateral walls of these subunits form regularly spaced bristle...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-06-01 00:00:00
abstract::Cysteine proteases are involved in the degradation of intracellular and extracellular proteins, although their precise roles in vivo are not well understood. Here we characterise a genetic mutant of the Caenorhabditis elegans cathepsin L protease gene cpl-1. CPL-1 is provided maternally and is essential for C. elegans...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01387
更新日期:2004-10-01 00:00:00
abstract::Dynamins are a conserved family of proteins involved in membrane fusion and fission. Although mammalian dynamins are known to be involved in several membrane-trafficking events, the role of dynamin-1 in endocytosis is the best-characterised role of this protein family. Despite many similarities between endocytosis in ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.070508
更新日期:2010-10-15 00:00:00
abstract::The alternative lengthening of telomeres (ALT) mechanism allows cancer cells to escape senescence and apoptosis in the absence of active telomerase. A characteristic feature of this pathway is the assembly of ALT-associated promyelocytic leukemia (PML) nuclear bodies (APBs) at telomeres. Here, we dissected the role of...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.148296
更新日期:2015-05-15 00:00:00
abstract::SNAREs such as VAMP, SNAP-25 and syntaxin are essential for intracellular trafficking, but what are their exact molecular roles and how are their interactions with other proteins manifest? Capitalizing on the differential sensitivity of SNAREs to exogenous proteases, we quantified the selective removal of identified S...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00374
更新日期:2003-05-15 00:00:00
abstract::SNARE [soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor] proteins control the membrane-fusion events of eukaryotic membrane-trafficking pathways. Specific vesicular and target SNAREs operate in specific trafficking routes, but the degree of specificity of SNARE functions is still elus...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.007948
更新日期:2007-09-15 00:00:00
abstract::The beta 2-tubulin isotype of Physarum polycephalum is only 83% identical in amino acid sequence with the constitutively expressed beta 1B-tubulin and the myxamoeba-specific beta 1A-tubulin isotypes. A polyclonal antibody specific for beta 2-tubulin was used to monitor the subcellular distribution of the beta 2-tubuli...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1989-10-01 00:00:00
abstract::Ovarian follicles are the basic functional units of female reproduction in the mammalian ovary. We show here that the protein a disintegrin and metalloproteinase domain 10 (ADAM10), a cell surface sheddase, plays an indispensable role in controlling primordial follicle formation by regulating the recruitment of follic...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.184267
更新日期:2016-06-01 00:00:00
abstract::We previously reported that, among the three connexins expressed in chick lens, overexpression of connexin (Cx) 45.6, not Cx43 or Cx56, stimulates lens cell differentiation; however, the underlying mechanism responsible for this effect is unclear. Here, we took advantage of naturally occurring loss-of-gap-junction fun...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.000935
更新日期:2007-10-15 00:00:00
abstract::In this paper, we identify the chondroitin sulfate proteoglycan versican V2 as a major inhibitor of axonal growth in the extracellular matrix of the mature central nervous system. In immunohistochemical and in situ hybridization experiments we show that this tissue-specific splice variant of versican is predominantly ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-03-01 00:00:00
abstract::Cells that have been pre-exposed to thermal stress can acquire a transient resistance against the killing effect of a subsequent thermal stress. The cause for this phenomenon, called thermotolerance, seems to be an enhanced resistance of proteins against thermal denaturation and aggregation. This resistance can be exp...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::The iron-storage protein ferritin was found to be associated with highly purified coated vesicles (CV) from chicken liver. Chicken liver ferritin was morphologically similar to ferritin from horse spleen and could be isolated using a specific anti-ferritin monoclonal antibody. This antibody recognized a 240 X 10(3) Mr...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::Epidermal growth factor receptor (EGFR) ligands undergo a complex series of processing events during their maturation to active signaling proteins. Like its mammalian homologs, the predominant Drosophila EGFR ligand Spitz is produced as a transmembrane pro-protein. In the secretory pathway, Spitz is cleaved within its...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.131169
更新日期:2013-10-01 00:00:00