Abstract：
:A mutation in the alpha 1-tubulin gene of Chlamydomonas reinhardtii was isolated by using the amiprophos-methyl-resistant mutation apm1-18 as a background to select new mutants that showed increased resistance to the drug. The upA12 mutation caused twofold resistance to amiprophos-methyl and oryzalin, and twofold hypersensitivity to the microtubule-stabilizing drug taxol, suggesting that the mutation enhanced microtubule stability. The resistance mutation was semi-dominant and mapped to the same interval on linkage group III as the alpha 1-tubulin gene. Two-dimensional gel immunoblots of proteins in the mutant cells revealed two electrophoretically altered alpha-tubulin isoforms, one of which was acetylated and incorporated into microtubules in the axoneme. The mutant isoforms co-segregated with the drug-resistance phenotypes when mutant upA12 was backcrossed to wild-type cells. Two-dimensional gel analysis of in vitro translation products showed that the non-acetylated variant alpha-tubulin was a primary gene product. DNA sequence analysis of the alpha 1-tubulin genes from mutant and wild-type cells revealed a single missense mutation, which predicted a change in codon 24 from tyrosine in wild type to histidine in mutant upA12. This alteration in the predicted amino acid sequence corroborated the approximately +1 basic charge shift observed for the variant alpha-tubulins. The mutant allele of the alpha 1-tubulin gene was designated tua1-1.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
James SW,Silflow CD,Stroom P,Lefebvre PAsubject
Has Abstractpub_date
1993-09-01 00:00:00pages
209-18eissn
0021-9533issn
1477-9137journal_volume
106 ( Pt 1)pub_type
杂志文章abstract：:The COP9 signalosome (CSN) is an evolutionarily conserved macromolecular complex that interacts with cullin-RING E3 ligases (CRLs) and regulates their activity by hydrolyzing cullin-Nedd8 conjugates. The CSN sequesters inactive CRL4(Ddb2), which rapidly dissociates from the CSN upon DNA damage. Here we systematically ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.043539
更新日期：2009-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2010-12-15 00:00:00
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journal_title：Journal of cell science
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更新日期：1995-02-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1998-09-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1999-11-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2016-03-01 00:00:00
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更新日期：2015-11-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1997-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1988-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2015-05-15 00:00:00
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journal_title：Journal of cell science
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更新日期：2017-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：2012-04-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1985-10-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03474
更新日期：2007-08-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.205492
更新日期：2017-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.036673
更新日期：2009-01-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2008-05-15 00:00:00
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pub_type： 杂志文章
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更新日期：2012-10-15 00:00:00
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pub_type： 杂志文章
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更新日期：2005-05-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2008-08-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1998-11-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
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更新日期：2013-06-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1975-08-01 00:00:00
