FAK and Src kinases are required for netrin-induced tyrosine phosphorylation of UNC5.

abstract：:Most secretory proteins travel through a well-documented conventional secretion pathway involving the endoplasmic reticulum (ER) and the Golgi complex. However, recently, it has been shown that a significant number of proteins reach the plasma membrane or extracellular space via unconventional routes. Unconventional p...

journal_title：Journal of cell science

authors： Kim J,Gee HY,Lee MG

更新日期：2018-06-25 00:00:00

abstract：:During mitosis, kinetochores need to attach to microtubules emanating from spindle poles. Several protein complexes have been shown to mediate the kinetochore-microtubule interaction. However, with the continually growing number of newly identified kinetochore proteins, it is unclear whether all major components of th...

journal_title：Journal of cell science

authors： Raaijmakers JA,Tanenbaum ME,Maia AF,Medema RH

更新日期：2009-07-15 00:00:00

abstract：:The mitotic spindle has long been recognized to play an essential role in determining the position of the cleavage furrow during cell division, however little is known about the mechanisms involved in this process. One attractive hypothesis is that signals from the spindle may function to induce reorganization of cort...

journal_title：Journal of cell science

authors： Fishkind DJ,Silverman JD,Wang YL

更新日期：1996-08-01 00:00:00

abstract：:Pin1/Ess1p is a highly conserved WW domain-containing peptidyl-prolyl isomerase (PPIase); its WW domain binds specifically to phospho-Ser/Thr-Pro sequences and its catalytic domain isomerizes phospho-Ser/Thr-Pro bonds. Pin1 PPIase activity can alter protein conformation in a phosphorylation-dependent manner and/or pro...

journal_title：Journal of cell science

authors： Huang HK,Forsburg SL,John UP,O'Connell MJ,Hunter T

更新日期：2001-10-01 00:00:00

abstract：:Prions are proteins that access self-templating amyloid forms, which confer phenotypic changes that can spread from individual to individual within or between species. These infectious phenotypes can be beneficial, as with yeast prions, or deleterious, as with mammalian prions that transmit spongiform encephalopathies...

journal_title：Journal of cell science

authors： Cushman M,Johnson BS,King OD,Gitler AD,Shorter J

更新日期：2010-04-15 00:00:00

abstract：:Paramecium tetraurelia is a unicellular organism that utilizes both axonemal and cytoplasmic dyneins. The highly conserved region containing the catalytic P-loop of the dynein heavy chain was amplified by RNA-directed polymerase chain reaction. Eight different P-loop-containing cDNA fragments were cloned. Southern hyb...

journal_title：Journal of cell science

authors： Asai DJ,Beckwith SM,Kandl KA,Keating HH,Tjandra H,Forney JD

更新日期：1994-04-01 00:00:00

abstract：:The conformation and biological activities of fibronectin (FN) and vitronectin (VN) coated on different plastic surfaces were investigated using cell adhesion and a panel of domain-specific monoclonal antibodies (mAbs). The adhesion of BHK fibroblasts was markedly better on FN coated on hydrophilic tissue culture poly...

journal_title：Journal of cell science

authors： Underwood PA,Steele JG,Dalton BA

更新日期：1993-03-01 00:00:00

abstract：:The water pipes of elongating plant organs are the result of programmed cell death and are formed by the walls of dead and empty protoxylem elements. These protoxylem elements are passively elongated many times by the surrounding tissue before they are replaced and collapse. Well-known adaptations for this unique task...

journal_title：Journal of cell science

authors： Ryser U,Schorderet M,Guyot R,Keller B

更新日期：2004-03-01 00:00:00

abstract：:Organelle transport is vital for the development and maintenance of axons, in which the distances between sites of organelle biogenesis, function, and recycling or degradation can be vast. Movement of mitochondria in axons can serve as a general model for how all organelles move: mitochondria are easy to identify, the...

journal_title：Journal of cell science

authors： Hollenbeck PJ,Saxton WM

更新日期：2005-12-01 00:00:00

abstract：:Uptake of cadmium into cultured cells and its effects on cell growth and DNA synthesis are measured over a range of Cd concentrations of seven orders of magnitude. Cd uptake is found to be proportional to the external Cd concentration and to incubation time over a very broad range of concentrations. At least 200 mmol ...

journal_title：Journal of cell science

authors： von Zglinicki T,Edwall C,Ostlund E,Lind B,Nordberg M,Ringertz NR,Wroblewski J

更新日期：1992-12-01 00:00:00

abstract：:The end1 mutant belongs to a group of four vacuolar protein sorting mutants (class C vps) that lack a morphologically distinguishable and functional vacuole. These mutants share several other phenotypes, such as the inability to grow at 37 degrees C or on nonfermentable carbon sources. We show that, as in the case of ...

journal_title：Journal of cell science

authors： Dulić V,Riezman H

更新日期：1990-11-01 00:00:00

abstract：:The primary structures of three human forms of secretory carrier membrane proteins (SCAMPs) have been deduced from full-length clones isolated from a HeLa cell cDNA library and confirmed by a combination of comparison to expressed sequence tags, microsequencing of purified protein, and in vitro transcription and trans...

journal_title：Journal of cell science

authors： Singleton DR,Wu TT,Castle JD

更新日期：1997-09-01 00:00:00

abstract：:Cytoplasmic dynein-2 is a motor protein complex that drives the movement of cargoes along microtubules within cilia, facilitating the assembly of these organelles on the surface of nearly all mammalian cells. Dynein-2 is crucial for ciliary function, as evidenced by deleterious mutations in patients with skeletal abno...

journal_title：Journal of cell science

authors： Vuolo L,Stevenson NL,Mukhopadhyay AG,Roberts AJ,Stephens DJ

更新日期：2020-03-30 00:00:00

abstract：:The mammalian retromer complex is a multi-protein complex that regulates retrograde transport of the cation-independent mannose 6-phosphate receptor (CI-MPR) from early endosomes to the trans Golgi network (TGN). It consists of two subcomplexes: a membrane-bound coat comprising sorting nexin-1 (SNX1) and possibly sort...

journal_title：Journal of cell science

authors： Bujny MV,Popoff V,Johannes L,Cullen PJ

更新日期：2007-06-15 00:00:00

abstract：:The serine/threonine protein phosphatase-1 (PP1) complex is a key regulator of the cell cycle. However, the redundancy of PP1 isoforms and the lack of specific inhibitors have hampered studies on the global role of PP1 in cell cycle progression in vertebrates. Here, we show that the overexpression of nuclear inhibitor...

journal_title：Journal of cell science

authors： Winkler C,De Munter S,Van Dessel N,Lesage B,Heroes E,Boens S,Beullens M,Van Eynde A,Bollen M

更新日期：2015-12-15 00:00:00

abstract：:Removal of interleukin-3 from murine IC.DP pre-mast cells results in irreversible commitment to apoptosis within 18 hours. To identify early events necessary for the engagement of apoptosis we examined the regulation of intracellular pH (pH(i)). IC.DP cells acidified 2 hours after removal of interleukin-3 (before disc...

journal_title：Journal of cell science

authors： Chen Q,Benson RS,Whetton AD,Brant SR,Donowitz M,Montrose MH,Dive C,Watson AJ

更新日期：1997-02-01 00:00:00

abstract：:Mutations to Pax3 and other Pax family genes in both mice and humans result in numerous tissue-specific morphological defects. Little is known, however, about the cellular and molecular mechanisms by which Pax genes regulate morphogenesis. We previously showed that Pax3 induces cell aggregation and a mesenchymal-to-ep...

journal_title：Journal of cell science

authors： Wiggan O,Hamel PA

更新日期：2002-02-01 00:00:00

abstract：:Fluorescence in situ hybridization has demonstrated that chromosomes form individual territories in interphase nuclei. However, this technique is not suitable to determine whether territories are mutually exclusive or interwoven. This notion, however, is essential for understanding functional organizations in the cell...

journal_title：Journal of cell science

authors： Visser AE,Aten JA

更新日期：1999-10-01 00:00:00

abstract：:In sciarid flies (Diptera, Sciaridae), one or two paternally derived X chromosomes are discarded from the soma at early cleavages to determine the sex of the embryo (XX, females; X0, males). X chromosome(s) elimination is achieved by an abnormal anaphase segregation so that X sister chromatids do not reach the poles a...

journal_title：Journal of cell science

authors： Escribá MC,Goday C

更新日期：2013-07-15 00:00:00

abstract：:Deubiquitylating (or deubiquitinating) enzymes (DUBs) are proteases that reverse protein ubiquitylation and therefore modulate the outcome of this post-translational modification. DUBs regulate a variety of intracellular processes, including protein turnover, signalling pathways and the DNA damage response. They have ...

journal_title：Journal of cell science

authors： Leznicki P,Kulathu Y

更新日期：2017-06-15 00:00:00

abstract：:Podosomes, adhesion structures capable of matrix degradation, have been linked with the ability of cells to perform chemotaxis and invade tissues. Wiskott-Aldrich Syndrome protein (WASp), an effector of the RhoGTPase Cdc42 and a Src family kinase substrate, regulates macrophage podosome formation. In this study, we de...

journal_title：Journal of cell science

authors： Dovas A,Gevrey JC,Grossi A,Park H,Abou-Kheir W,Cox D

更新日期：2009-11-01 00:00:00

abstract：:Cytoplasm of Paramecium caudatum syngen 3 in the period of sexual immaturity was effective in inducing immaturity when injected into mating-reactive cells of other syngens of P. caudatum. When immature cytoplasm of P. caudatum was injected into mature cells of P. multimicronucleatum, immaturity was also induced in the...

journal_title：Journal of cell science

authors： Miwa I

更新日期：1979-04-01 00:00:00

abstract：:In hepatocytes, PLIN2 is the major protein coating lipid droplets (LDs), an organelle the hepatitis C virus (HCV) hijacks for virion morphogenesis. We investigated the consequences of PLIN2 deficiency on LDs and on HCV infection. Knockdown of PLIN2 did not affect LD homeostasis, likely due to compensation by PLIN3, bu...

journal_title：Journal of cell science

authors： Lassen S,Grüttner C,Nguyen-Dinh V,Herker E

更新日期：2019-01-09 00:00:00

abstract：:In chicken oocytes, proteins of the K/J family or their analogs, such as are known to be involved in mRNA processing in humans, are closely associated with nascent C-rich RNA transcripts on the loops of lampbrush chromosomes. Using labelled single stranded nucleotide probes and an antibody to protein K, these C-rich t...

journal_title：Journal of cell science

authors： Solovei I,Macgregor H,Gaginskaya E

更新日期：1995-04-01 00:00:00

abstract：:In invertebrates, UNC-45 regulates myosin stability and functions. Vertebrates have two distinct isoforms of the protein: UNC-45B, expressed in muscle cells only, and UNC-45A, expressed in all cells and implicated in regulating both non-muscle myosin II (NMII)- and microtubule (MT)-associated functions. Here, we show ...

journal_title：Journal of cell science

authors： Habicht J,Mooneyham A,Hoshino A,Shetty M,Zhang X,Emmings E,Yang Q,Coombes C,Gardner MK,Bazzaro M

更新日期：2021-01-08 00:00:00

abstract：:Low-intensity pulsed ultrasound (LIPUS) is a therapy used clinically to promote healing. Using live-cell imaging we show that LIPUS stimulation, acting through integrin-mediated cell-matrix adhesions, rapidly induces Rac1 activation associated with dramatic actin cytoskeleton rearrangements. Our study demonstrates tha...

journal_title：Journal of cell science

authors： Atherton P,Lausecker F,Harrison A,Ballestrem C

更新日期：2017-07-15 00:00:00

abstract：:Neurite elongation and branching are key cellular events during brain development as they underlie the formation of a properly wired neuronal network. Here we report that the receptor tyrosine kinases Ror1 and Ror2 modulate the growth of neurites as well as their branching pattern in hippocampal neurons. Upon Ror1 or ...

journal_title：Journal of cell science

authors： Paganoni S,Ferreira A

更新日期：2005-01-15 00:00:00

abstract：:High-throughput neurotransmission at ribbon synapses of cochlear inner hair cells (IHCs) requires tight coupling of neurotransmitter release and balanced recycling of synaptic vesicles (SVs) as well as rapid restoration of release sites. Here, we examined the role of the adaptor protein AP180 (also known as SNAP91) fo...

journal_title：Journal of cell science

authors： Kroll J,Özçete ÖD,Jung S,Maritzen T,Milosevic I,Wichmann C,Moser T

更新日期：2020-01-22 00:00:00

abstract：:Cells are internally organized into compartmentalized organelles that execute specialized functions. To understand the functions of individual organelles and their regulations, it is critical to resolve the compositions of individual organelles, which relies on a rapid and efficient isolation method for specific organ...

journal_title：Journal of cell science

authors： Xiong J,He J,Xie WP,Hinojosa E,Ambati CSR,Putluri N,Kim HE,Zhu MX,Du G

更新日期：2019-12-13 00:00:00

abstract：:When migrating mesenchymal cells collide, they exhibit a 'contact inhibition of locomotion' response that results in reversal of their front-rear polarity by extension of a new leading edge, which enables their migration away from the opposing contacted cell. The critical cytoskeletal rearrangements underpinning these...

journal_title：Journal of cell science

authors： Kadir S,Astin JW,Tahtamouni L,Martin P,Nobes CD

更新日期：2011-08-01 00:00:00