Drebrin preserves endothelial integrity by stabilizing nectin at adherens junctions.


:Regulation of cell-cell contacts is essential for integrity of the vascular endothelium. Here, a critical role of the F-actin-binding protein drebrin in maintaining endothelial integrity is revealed under conditions mimicking vascular flow. Drebrin knockdown leads to weakening of cell-cell contacts, characterized by loss of nectin from adherens junctions and its subsequent lysosomal degradation. Immunoprecipitation, FRAP and mitochondrial re-targeting experiments show that nectin stabilization occurs through a chain of interactions: drebrin binding to F-actin, interaction of drebrin and afadin through their polyproline and PR1-2 regions, and recruitment of nectin through the PDZ region of afadin. Key elements are modules in drebrin that confer binding to afadin and F-actin. Evidence for this was obtained using constructs containing the PDZ region of afadin coupled to the F-actin-binding region of drebrin or to lifeact, which restore junctional nectin under knockdown of drebrin or of both drebrin and afadin. Drebrin, containing binding sites for both afadin and F-actin, is thus uniquely equipped to stabilize nectin at endothelial junctions and to preserve endothelial integrity under vascular flow.


J Cell Sci


Journal of cell science


Rehm K,Panzer L,van Vliet V,Genot E,Linder S




Has Abstract


2013-08-15 00:00:00




Pt 16










  • Altered patterns of ribonucleic acid synthesis during the cell cycle: a mechanism compensating for variation in gene concentration.

    abstract::In the fission yeast Schizosaccharomyces pombe, a series of diploid mutants divides at smaller cell sizes than wild type. In these smaller strains, the mean gene concentration (defined by previous authors as the DNA to protein ratio) is higher than in wild type. Such an increase in gene concentration should also incre...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Fraser RS,Nurse P

    更新日期:1979-02-01 00:00:00

  • Polarity of endogenous and exogenous glycosyl-phosphatidylinositol-anchored membrane proteins in Madin-Darby canine kidney cells.

    abstract::Madin-Darby canine kidney cells (MDCK) were transfected with a cDNA encoding the glycosyl-phosphatidylinositol (GPI)-anchored protein mouse Thy-1 in order to study the steady-state surface distribution of exogenous and endogenous GPI-linked proteins. Immunofluorescence of transfected cells grown on collagen-coated cov...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Wilson JM,Fasel N,Kraehenbuhl JP

    更新日期:1990-05-01 00:00:00

  • The role of annexin 2 in osteoblastic mineralization.

    abstract::While the basic cellular contributions to bone differentiation and mineralization are widely accepted, the regulation of these processes at the intracellular level remains inadequately understood. Our laboratory recently identified annexin 2 as a protein involved in osteoblastic mineralization. Annexin 2 was overexpre...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Gillette JM,Nielsen-Preiss SM

    更新日期:2004-01-26 00:00:00

  • Locomotion of Xenopus epidermis cells in primary culture.

    abstract::The locomotion of single epidermis cells, grown out from Xenopus laevis tadpole tails has been investigated by time-lapse cinemicrography using phase-contrast and reflection-contrast optics. The cells develop a large, mostly 200-250 nm thick, lamella, which adheres homogeneously to the supporting coverglass and exceed...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Bereiter-Hahn J,Strohmeier R,Kunzenbacher I,Beck K,Vöth M

    更新日期:1981-12-01 00:00:00

  • A molecular link A molecular link between Hairless and Pros26.4, a member of the AAA-ATPase subunits of the proteasome 19S regulatory particle in Drosophila.

    abstract::The proteasome is the major degradation machinery of the cell that regulates multiple cellular processes as diverse as cell cycle, signal transduction and gene expression. Recognition and unfolding of target proteins involves the regulatory cap whose base contains six AAA-ATPases that display reverse chaperone activit...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Müller D,Nagel AC,Maier D,Preiss A

    更新日期:2006-01-15 00:00:00

  • AP180 promotes release site clearance and clathrin-dependent vesicle reformation in mouse cochlear inner hair cells.

    abstract::High-throughput neurotransmission at ribbon synapses of cochlear inner hair cells (IHCs) requires tight coupling of neurotransmitter release and balanced recycling of synaptic vesicles (SVs) as well as rapid restoration of release sites. Here, we examined the role of the adaptor protein AP180 (also known as SNAP91) fo...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Kroll J,Özçete ÖD,Jung S,Maritzen T,Milosevic I,Wichmann C,Moser T

    更新日期:2020-01-22 00:00:00

  • A Ubc7p-binding domain in Cue1p activates ER-associated protein degradation.

    abstract::Cue1p is an N-terminally anchored endoplasmic reticulum (ER) protein essential for the activity of the two major yeast RING finger ubiquitin ligases (E3s) implicated in ER-associated degradation (ERAD). Cue1p contains a CUE domain, which for several proteins is known to bind ubiquitin. We now establish that the CUE do...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Kostova Z,Mariano J,Scholz S,Koenig C,Weissman AM

    更新日期:2009-05-01 00:00:00

  • Proteolytically cleaved MLL subunits are susceptible to distinct degradation pathways.

    abstract::The mixed lineage leukemia (MLL) proto-oncogenic protein is a histone-lysine N-methyltransferase that is produced by proteolytic cleavage and self-association of the respective functionally distinct subunits (MLL(N) and MLL(C)) to form a holocomplex involved in epigenetic transcriptional regulation. On the basis of st...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Yokoyama A,Ficara F,Murphy MJ,Meisel C,Naresh A,Kitabayashi I,Cleary ML

    更新日期:2011-07-01 00:00:00

  • Evidence for separate ND10-binding and homo-oligomerization domains of Sp100.

    abstract::Nuclear domains called ND10 or PML nuclear bodies consist of an aggregation of several proteins, most notably PML and Sp100. PML is essential in the nucleation and formation of ND10 as well as in the recruitment of other ND10-associated proteins such as Daxx, pRb, BLM and Sp100. In cells induced to overexpress Sp100, ...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Negorev D,Ishov AM,Maul GG

    更新日期:2001-01-01 00:00:00

  • Effects of Charcot-Marie-Tooth-linked mutations of the neurofilament light subunit on intermediate filament formation.

    abstract::Neurofilaments (NFs) are the major intermediate filaments (IFs) of mature neurons. They play important roles in the structure and function of axons. Recently, two mutations in the neurofilament light (NFL) subunit have been identified in families affected by Charcot-Marie-Tooth (CMT) neuropathy type 2. We have charact...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Perez-Olle R,Leung CL,Liem RK

    更新日期:2002-12-15 00:00:00

  • Intermediate filament formation after transfection with modified hamster vimentin and desmin genes.

    abstract::Previously we cloned and characterized the hamster intermediate filament genes coding for vimentin and desmin. It was demonstrated that the cloned desmin gene was expressed after gene transfer and that the newly synthesized protein assembles into intermediate filaments. Here we present data on the transfection of modi...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: van den Heuvel RM,van Eys GJ,Ramaekers FC,Quax WJ,Vree Egberts WT,Schaart G,Cuypers HT,Bloemendal H

    更新日期:1987-11-01 00:00:00

  • Stuck in reverse: loss of LC1 in Trypanosoma brucei disrupts outer dynein arms and leads to reverse flagellar beat and backward movement.

    abstract::Axonemal dyneins are multisubunit molecular motors that provide the driving force for flagellar motility. Dynein light chain 1 (LC1) has been well studied in Chlamydomonas reinhardtii and is unique among all dynein components as the only protein known to bind directly to the catalytic motor domain of the dynein heavy ...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Baron DM,Kabututu ZP,Hill KL

    更新日期:2007-05-01 00:00:00

  • RIF1 controls replication initiation and homologous recombination repair in a radiation dose-dependent manner.

    abstract::RIF1 controls both DNA replication timing and the DNA double-strand break (DSB) repair pathway to maintain genome integrity. However, it remains unclear how RIF1 links these two processes following exposure to ionizing radiation (IR). Here, we show that inhibition of homologous recombination repair (HRR) by RIF1 occur...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Saito Y,Kobayashi J,Kanemaki MT,Komatsu K

    更新日期:2020-06-22 00:00:00

  • Ultrastructural morphometric of gap junctions during differentiation of stratified squamous epithelium.

    abstract::The presence of gap junctions in stratified epithelia has now been extensively documented, but there have been few attempts to quantify them. In the present report, samples of hamster cheek pouch mucosa were processed for electron microscopy and electron micrographs from defined basal, spinous and granular layers were...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: White FH,Thompson DA,Gohari K

    更新日期:1984-07-01 00:00:00

  • Nonprimed and DYRK1A-primed GSK3 beta-phosphorylation sites on MAP1B regulate microtubule dynamics in growing axons.

    abstract::MAP1B is a developmentally regulated microtubule-associated phosphoprotein that regulates microtubule dynamics in growing axons and growth cones. We used mass spectrometry to map 28 phosphorylation sites on MAP1B, and selected for further study a putative primed GSK3 beta site and compared it with two nonprimed GSK3 b...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Scales TM,Lin S,Kraus M,Goold RG,Gordon-Weeks PR

    更新日期:2009-07-15 00:00:00

  • Differential detergent resistance of the apical and basolateral NPPases: relationship with polarized targeting.

    abstract::Targeting of glycosylphosphatidylinositol-anchored proteins to the apical surface of epithelial cells involves clustering in Triton X-100-resistant membrane microdomains or rafts. The role of these microdomains in sorting transmembrane proteins is more questionable because, unlike glycosylphosphatidylinositol-anchored...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Delaunay JL,Breton M,Goding JW,Trugnan G,Maurice M

    更新日期:2007-03-15 00:00:00

  • ERBIN is a new SARA-interacting protein: competition between SARA and SMAD2 and SMAD3 for binding to ERBIN.

    abstract::SARA, an early endosomal protein, plays a key role in TGFβ signalling, as it presents SMAD2 and SMAD3 for phosphorylation by the activated TGFβ receptors. Here, we show that ERBIN is a new SARA-interacting protein that can be recruited by SARA to early endosomes. ERBIN was recently shown to bind and segregate phosphor...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Sflomos G,Kostaras E,Panopoulou E,Pappas N,Kyrkou A,Politou AS,Fotsis T,Murphy C

    更新日期:2011-10-01 00:00:00

  • Tumour necrosis factor alpha confers an invasive, transformed phenotype on mammary epithelial cells.

    abstract::Although loss of cell-cell adhesion and gain of invasive properties play a crucial role in the malignant progression of epithelial tumours, the molecular signals that trigger these processes have not been fully elucidated. In light of the well-established relationship between chronic inflammation and cancer, we hypoth...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Montesano R,Soulié P,Eble JA,Carrozzino F

    更新日期:2005-08-01 00:00:00

  • Inhibitors of the V0 subunit of the vacuolar H+-ATPase prevent segregation of lysosomal- and secretory-pathway proteins.

    abstract::The vacuolar H(+)-ATPase (V-ATPase) establishes pH gradients along secretory and endocytic pathways. Progressive acidification is essential for proteolytic processing of prohormones and aggregation of soluble content proteins. The V-ATPase V(0) subunit is thought to have a separate role in budding and fusion events. P...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Sobota JA,Bäck N,Eipper BA,Mains RE

    更新日期:2009-10-01 00:00:00

  • Role of zinc in cell division of Euglena gracilis.

    abstract::Euglena gracilis is a suitable model system to investigate the role of zinc in the process of cell division. In zinc-deficient organisms there is a characteristic arrest of cellular proliferation, the DNA content of the cells doubles, whereas RNA and protein contents decrease. The present investigations include the gr...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Falchuk KH,Fawcett DW,Vallee BL

    更新日期:1975-01-01 00:00:00

  • Radiation-induced mitotic catastrophe in PARG-deficient cells.

    abstract::Poly(ADP-ribosyl)ation is a post-translational modification of proteins involved in the regulation of chromatin structure, DNA metabolism, cell division and cell death. Through the hydrolysis of poly(ADP-ribose) (PAR), Poly(ADP-ribose) glycohydrolase (PARG) has a crucial role in the control of life-and-death balance f...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Amé JC,Fouquerel E,Gauthier LR,Biard D,Boussin FD,Dantzer F,de Murcia G,Schreiber V

    更新日期:2009-06-15 00:00:00

  • A RhoG-mediated signaling pathway that modulates invadopodia dynamics in breast cancer cells.

    abstract::One of the hallmarks of cancer is the ability of tumor cells to invade surrounding tissues and metastasize. During metastasis, cancer cells degrade the extracellular matrix, which acts as a physical barrier, by developing specialized actin-rich membrane protrusion structures called invadopodia. The formation of invado...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Goicoechea SM,Zinn A,Awadia SS,Snyder K,Garcia-Mata R

    更新日期:2017-03-15 00:00:00

  • Fc-receptor-mediated phagocytosis is regulated by mechanical properties of the target.

    abstract::Phagocytosis is an actin-based process used by macrophages to clear particles greater than 0.5 microm in diameter. In addition to its role in immunological responses, phagocytosis is also necessary for tissue remodeling and repair. To prevent catastrophic autoimmune reactions, phagocytosis must be tightly regulated. I...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Beningo KA,Wang YL

    更新日期:2002-02-15 00:00:00

  • The scavenger receptor CD36 plays a role in cytokine-induced macrophage fusion.

    abstract::Multinucleated giant cells, characteristic of granulomatous infections, originate from the fusion of macrophages. Using an antibody screening strategy we found that the scavenger receptor CD36 participates in macrophage fusion induced by the cytokines IL-4 and GM-CSF. Our results demonstrate that exposure of phosphati...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Helming L,Winter J,Gordon S

    更新日期:2009-02-15 00:00:00

  • Involvement of intracellular calcium in protein secretion in Dictyostelium discoideum.

    abstract::We reported previously that Ca2+ depletion of Dictyostelium discoideum cells severely inhibits extracellular cyclic nucleotide phosphodiesterase (PD) synthesis at a post-transcriptional step. In this study, further experiments were performed to learn more about the nature of this phenomenon. Examination of the polysom...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Coukell MB,Cameron AM,Adames NR

    更新日期:1992-10-01 00:00:00

  • Cell contact-induced inhibition of division in Dictyostelium.

    abstract::The developmental stage at which Dictyostelium discoideum strain v12/M2 cells was inhibited in resuming cell division was monitored by a Coulter counter. Neither vegetative nor pre-aggregating v12/M2 amoebae used as inocula in liquid growth cultures exhibited a delay in recommencing cell division. Inocula prepared fro...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Kopachik WJ,Gregg JH

    更新日期:1978-02-01 00:00:00

  • Plasmodium falciparum apical membrane antigen 1 (PfAMA-1) is translocated within micronemes along subpellicular microtubules during merozoite development.

    abstract::During the assembly of Plasmodium falciparum merozoites within the schizont stage, the parasite synthesizes and positions three sets of secretory vesicles (rhoptries, micronemes and dense granules) that are active during red cell invasion. There are up to 40 micronemes per merozoite, shaped like long-necked bottles, a...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Bannister LH,Hopkins JM,Dluzewski AR,Margos G,Williams IT,Blackman MJ,Kocken CH,Thomas AW,Mitchell GH

    更新日期:2003-09-15 00:00:00

  • Differential localization of WAVE isoforms in filopodia and lamellipodia of the neuronal growth cone.

    abstract::The formation and extension of filopodia in response to an extracellular stimulus by guidance cues determine the path of growth cone advance. Actin-filament bundling and actin polymerization at the tips supply the driving force behind the formation and elongation. We tried to clarify how signals in response to extrace...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Nozumi M,Nakagawa H,Miki H,Takenawa T,Miyamoto S

    更新日期:2003-01-15 00:00:00

  • The role of mechanical stimuli in the vascular differentiation of mesenchymal stem cells.

    abstract::Mesenchymal stem cells (MSCs) are among the most promising and suitable stem cell types for vascular tissue engineering. Substantial effort has been made to differentiate MSCs towards vascular cell phenotypes, including endothelial cells and smooth muscle cells (SMCs). The microenvironment of vascular cells not only c...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审


    authors: Dan P,Velot É,Decot V,Menu P

    更新日期:2015-07-15 00:00:00

  • mAKAP: an A-kinase anchoring protein targeted to the nuclear membrane of differentiated myocytes.

    abstract::The compartmentalization of second messenger-activated protein kinases contributes to the fidelity of hormone-mediated signal transduction events. For example, the cAMP-dependent protein kinase is tethered at specific intracellular locations through association with A-kinase anchoring proteins (AKAPs). We now report t...

    journal_title:Journal of cell science

    pub_type: 杂志文章


    authors: Kapiloff MS,Schillace RV,Westphal AM,Scott JD

    更新日期:1999-08-01 00:00:00