Drebrin preserves endothelial integrity by stabilizing nectin at adherens junctions.

Abstract:

:Regulation of cell-cell contacts is essential for integrity of the vascular endothelium. Here, a critical role of the F-actin-binding protein drebrin in maintaining endothelial integrity is revealed under conditions mimicking vascular flow. Drebrin knockdown leads to weakening of cell-cell contacts, characterized by loss of nectin from adherens junctions and its subsequent lysosomal degradation. Immunoprecipitation, FRAP and mitochondrial re-targeting experiments show that nectin stabilization occurs through a chain of interactions: drebrin binding to F-actin, interaction of drebrin and afadin through their polyproline and PR1-2 regions, and recruitment of nectin through the PDZ region of afadin. Key elements are modules in drebrin that confer binding to afadin and F-actin. Evidence for this was obtained using constructs containing the PDZ region of afadin coupled to the F-actin-binding region of drebrin or to lifeact, which restore junctional nectin under knockdown of drebrin or of both drebrin and afadin. Drebrin, containing binding sites for both afadin and F-actin, is thus uniquely equipped to stabilize nectin at endothelial junctions and to preserve endothelial integrity under vascular flow.

journal_name

J Cell Sci

journal_title

Journal of cell science

authors

Rehm K,Panzer L,van Vliet V,Genot E,Linder S

doi

10.1242/jcs.129437

subject

Has Abstract

pub_date

2013-08-15 00:00:00

pages

3756-69

issue

Pt 16

eissn

0021-9533

issn

1477-9137

pii

jcs.129437

journal_volume

126

pub_type

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