Abstract：
:It has been suggested by many studies that Ca2+ signaling plays an important role in regulating key steps in cell division. In order to study the down stream components of calcium signaling, we have fused the gene of calmodulin (CaM) with that of green fluorescent protein (GFP) and expressed it in HeLa cells. The GFP-CaM protein was found to have similar biochemical properties as the wild-type CaM, and its distribution was also similar to that of the endogenous CaM. Using this GFP-tagged CaM as a probe, we have conducted a detailed examination of the spatial- and temporal-dependent redistribution of calmodulin in living mammalian cells during cell division. Our major findings are: (1) high density of CaM was found to distribute in two sub-cellular locations during mitosis; one fraction was concentrated in the spindle poles, while the other was concentrated in the sub-membrane region around the cell. (2) The sub-membrane fraction of CaM became aggregated at the equatorial region where the cleavage furrow was about to form. The timing of this localized aggregation of CaM was closely associated with the onset of cytokinesis. (3) Using a TA-CaM probe, we found that the sub-membrane fraction of CaM near the cleavage furrow was selectively activated during cell division. (4) When we injected a CaM-specific inhibitory peptide into early anaphase cells, cytokinesis was either blocked or severely delayed. These findings suggest that, in addition to Ca2+ ion, CaM may represent a second signal that can also play an active role in determining the positioning and timing of the cleavage furrow formation.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Li CJ,Heim R,Lu P,Pu Y,Tsien RY,Chang DCkeywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
1567-77eissn
0021-9533issn
1477-9137journal_volume
112 ( Pt 10)pub_type
杂志文章abstract：:The neuroprotective role of carbon monoxide (CO) has been studied in a cell-autonomous mode. Herein, a new concept is disclosed - CO affects astrocyte-neuron communication in a paracrine manner to promote neuroprotection. Neuronal survival was assessed when co-cultured with astrocytes that had been pre-treated or not ...
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journal_title：Journal of cell science
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更新日期：1983-11-01 00:00:00
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更新日期：1998-06-01 00:00:00
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更新日期：2002-11-01 00:00:00
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更新日期：2005-08-01 00:00:00
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更新日期：2008-02-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1998-10-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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更新日期：1988-11-01 00:00:00
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更新日期：1978-08-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1998-01-01 00:00:00
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journal_title：Journal of cell science
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pub_type： 杂志文章
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更新日期：1999-12-01 00:00:00
