Abstract:
:The C terminus of the tubulin alpha-subunit of most eukaryotic cells undergoes a cycle of tyrosination and detyrosination using two specific enzymes, a tubulin tyrosine ligase (TTL) and a tubulin carboxypeptidase. Although this enzyme cycle is conserved in evolution and exhibits rapid turnover, the meaning of this modification has remained elusive. We have isolated several NIH-3T3 derived clonal cell lines that lack TTL (TTL-). TTL- cells contain a unique tubulin isotype (delta2-tubulin) that can be detected with specific antibodies. When injected into nude mice, both TTL- cells and TTL- cells stably transfected with TTL cDNA form sarcomas. But in tumors formed from TTL rescued cells, TTL is systematically lost during tumor growth. A strong selection process has thus acted during tumor growth to suppress TTL activity. In accord with this result, we find suppression of TTL activity in the majority of human tumors assayed with delta2-tubulin antibody. We conclude there is a widespread loss of TTL activity during tumor growth in situ, suggesting that TTL activity may play a role in tumor cell regulation.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Lafanechère L,Courtay-Cahen C,Kawakami T,Jacrot M,Rüdiger M,Wehland J,Job D,Margolis RLsubject
Has Abstractpub_date
1998-01-01 00:00:00pages
171-81eissn
0021-9533issn
1477-9137journal_volume
111 ( Pt 2)pub_type
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1995-02-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2012-11-01 00:00:00
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journal_title:Journal of cell science
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更新日期:1996-03-01 00:00:00
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更新日期:2000-03-01 00:00:00
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journal_title:Journal of cell science
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