Abstract：
:How ion channels localize and distribute on the cell membrane remains incompletely understood. We show that interventions that vary cell adhesion proteins and cell size also affect the membrane current density of inward-rectifier K+ channels (Kir2.1; encoded by KCNJ2) and profoundly alter the action potential shape of excitable cells. By using micropatterning to manipulate the localization and size of focal adhesions (FAs) in single HEK293 cells engineered to stably express Kir2.1 channels or in neonatal rat cardiomyocytes, we establish a robust linear correlation between FA coverage and the amplitude of Kir2.1 current at both the local and whole-cell levels. Confocal microscopy showed that Kir2.1 channels accumulate in membrane proximal to FAs. Selective pharmacological inhibition of key mediators of protein trafficking and the spatially dependent alterations in the dynamics of Kir2.1 fluorescent recovery after photobleaching revealed that the Kir2.1 channels are transported to the cell membrane uniformly, but are preferentially internalized by endocytosis at sites that are distal from FAs. Based on these results, we propose adhesion-regulated membrane localization of ion channels as a fundamental mechanism of controlling cellular electrophysiology via mechanochemical signals, independent of the direct ion channel mechanogating.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Sengupta S,Rothenberg KE,Li H,Hoffman BD,Bursac Ndoi
10.1242/jcs.225383subject
Has Abstractpub_date
2019-09-02 00:00:00issue
17eissn
0021-9533issn
1477-9137pii
jcs.225383journal_volume
132pub_type
杂志文章abstract：:The current model for hemoglobin ingestion and transport by intraerythrocytic Plasmodium falciparum malaria parasites shares similarities with endocytosis. However, the model is largely hypothetical, and the mechanisms responsible for the ingestion and transport of host cell hemoglobin to the lysosome-like food vacuol...
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journal_title：Journal of cell science
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abstract：:Multivesicular endosomes are present in virtually every eucaryotic cell, where they arise by intra-endosomal budding of the limiting endosomal membrane. Some genetic diseases such as Chediak-Higashi syndrome are characterized by enlarged membrane-filled endosomes. The same altered endosomal morphology can be observed ...
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doi：10.1242/jcs.01524
更新日期：2004-12-01 00:00:00
abstract：:Annexin 1 is a Ca(2+)-regulated membrane binding protein and a major substrate of the epidermal growth factor receptor kinase. Because of its properties and intracellular distribution, the protein has been implicated in endocytic trafficking of the receptor, in particular in receptor sorting occurring in multivesicula...
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更新日期：2011-11-15 00:00:00
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doi：
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.152520
更新日期：2014-11-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.051326
更新日期：2010-02-15 00:00:00
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journal_title：Journal of cell science
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doi：
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.041178
更新日期：2009-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.246199
更新日期：2020-10-12 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03272
更新日期：2006-12-01 00:00:00
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doi：
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2005-10-01 00:00:00
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journal_title：Journal of cell science
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doi：
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doi：
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journal_title：Journal of cell science
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更新日期：2008-02-01 00:00:00
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更新日期：2020-09-11 00:00:00
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abstract：:Epidermal growth factor receptor has been localized to the proliferative cell layers in a variety of stratified squamous epithelia. In the current study, the rat cornea was used as an experimental model to determine if epidermal growth factor receptor is concentrated in epithelial stem cells. Epidermal growth factor r...
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doi：
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doi：
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journal_title：Journal of cell science
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更新日期：1978-06-01 00:00:00
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