Abstract:
:Serial sections of mitotic spindles of the marine cryptophycean alga, Cryptomonas, were analysed to determine what types of microtubules they contained and which of these microtubules came close enough to each other (50 nm or less) for the commonly described crossbridging to be possible. Interpolar microtubules were rare (less than or equal to 1%) but from prometaphase through anaphase there was a substantial interpolar framework of free and polar microtubules which came close enough to one another to cross-bridge and generate anaphase spindle elongation by intermicrotubule sliding. However, such elongation would also require some concomitant polar microtubule polymerization. In contrast, only about 12% of the chromosomal microtubules came within bridging distance of interpolar framework microtubules. Thus, assuming that microtubules were accurately fixed in their in vivo positions, crossbridging between chromosomal and interpolar framework microtubules is unlikely to function in chromosome-to-pole movement. In all stages the great majority of chromosomal microtubules were found to extend all the way from the chromatin to the pole. Although the identification of intermicrotubular bridges in section is often ambiguous, the best and most frequent examples of bridges in the present material were between polar microtubules and between chromosomal microtubules but not between the 2 different categories. The spindles also contained 5-nm-diameter microfilaments associated mainly with chromosomal microtubules and occasionally with polar microtubules. A specific model for the possible involvement of these filaments in mitosis is presented.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Oakley BR,Heath IBsubject
Has Abstractpub_date
1978-06-01 00:00:00pages
53-70eissn
0021-9533issn
1477-9137journal_volume
31pub_type
杂志文章abstract::The Arp2/3 complex is important for morphogenesis in various developmental systems, but specific in vivo roles for this complex in cells that move during morphogenesis are not well understood. We have examined cellular roles for Arp2/3 in the Caenorhabditis elegans embryo. In C. elegans, the first morphogenetic moveme...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.057562
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journal_title:Journal of cell science
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abstract::The role of basal epithelial cells in prostatic function, development and carcinogenesis is unknown. The ability of basal prostatic epithelial cells to acquire a luminal phenotype was explored in vitro using the NRP-152 rat dorsal-lateral prostate epithelial cell line as a model system. NRP-152, which was spontaneousl...
journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.207316
更新日期:2018-01-04 00:00:00
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.216630
更新日期:2018-09-03 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.033878
更新日期:2008-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
abstract::The large GTPase dynamin, long known for its role in endocytosis, has most recently been implicated as a facilitator of cell migration and invasion. Recent observations link dynamin to the cycle of membrane expansion and retraction essential for cell motility. Its role in actin polymerization, membrane deformation and...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.02963
更新日期:2006-05-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03378
更新日期:2007-03-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.239673
更新日期:2020-06-23 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.228072
更新日期:2020-04-07 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.131169
更新日期:2013-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2012-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2004-10-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-03-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2015-04-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2019-03-07 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2005-03-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
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journal_title:Journal of cell science
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更新日期:2002-08-15 00:00:00