Abstract：
:We show that muscle satellite cells, traditionally considered as committed myogenic precursors, are comprised of Pax7-expressing progenitors that preserve a mesenchymal repertoire extending beyond a mere myogenic potential. Mouse satellite cells from freshly isolated single myofibers, cultured individually in serum-rich growth medium, produced myogenic and non-myogenic clones. Only the myogenic clones expressed muscle-specific transcription factors and formed myotubes. Pax7 was initially expressed in all clones, but subsequently was associated only with the myogenic clones. Some cells in the non-myogenic clones expressed alpha-smooth muscle actin and nestin whereas others differentiated into mature adipocytes. This type of cell composition mirrors characteristics of mesenchymal stem cell progeny. Overall, individual myofibers persistently gave rise to both clonal phenotypes, but the ratio of myogenic to non-myogenic clones randomly varied among fibers. This randomness indicates that clonal dichotomy reflects satellite cell suppleness rather than pre-fated cell heterogeneity. We conclude that satellite cells possess mesenchymal plasticity, being able to commit either to myogenesis or to a mesenchymal alternative differentiation (MAD) program.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Shefer G,Wleklinski-Lee M,Yablonka-Reuveni Zdoi
10.1242/jcs.01419keywords:
subject
Has Abstractpub_date
2004-10-15 00:00:00pages
5393-404issue
Pt 22eissn
0021-9533issn
1477-9137pii
jcs.01419journal_volume
117pub_type
杂志文章abstract：:The distribution of centromeres in the interphase nuclei of human diploid fibroblasts was analyzed using anti-centromere immunofluorescence and laser scanning confocal microscopoy. The positions of the centromeres were placed within the nuclear chromatin distribution and presented some aspects of the dynamics of nucle...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:Neurite elongation and branching are key cellular events during brain development as they underlie the formation of a properly wired neuronal network. Here we report that the receptor tyrosine kinases Ror1 and Ror2 modulate the growth of neurites as well as their branching pattern in hippocampal neurons. Upon Ror1 or ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01622
更新日期：2005-01-15 00:00:00
abstract：:The effects of hyaluronate on the morphology, motile and social behaviour of embryonic chick heart fibroblasts were examined in the presence or absence of hyaluronate binding proteins (HABP). HABP were inserted onto fibroblasts after treating cells with 0.2 M-urea. Such treatment increased the binding of [125I]HABP to...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1985-10-01 00:00:00
abstract：:Brain-derived neurotrophic factor (BDNF) is known to be a crucial regulator of neuronal survival and synaptic plasticity in the mammalian brain. Furthermore, BDNF positively influences differentiation of embryonic neural precursors, as well as that of neural stem cells from adult neurogenic niches. To study the impact...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.135384
更新日期：2013-11-01 00:00:00
abstract：:This project examines the transcellular membrane protein polarity of bovine aortic endothelial cell (BAEC) monolayers in vitro with respect to the roles that intercellular junctions (as defined by comparing confluent and subconfluent monolayers) and the submembranous cytoskeleton play in controlling this phenomenon. P...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：:Genomes exist in vivo as complex physical structures, and their functional output (i.e. the gene expression profile of a cell) is related to their spatial organization inside the nucleus as well as to local chromatin status. Chromatin modifications and chromosome conformation are distinct in different tissues and cell...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.051631
更新日期：2010-06-15 00:00:00
abstract：:ABCG2 belongs to the ATP-binding cassette superfamily of transmembrane proteins and is ubiquitously expressed in stem cells including those in the developing nervous system. The ability of ABCG2 to preferentially exclude DNA-intercalating dyes is regarded to be the basis for the enrichment of stem cells or progenitors...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.008417
更新日期：2007-08-01 00:00:00
abstract：:Cells are internally organized into compartmentalized organelles that execute specialized functions. To understand the functions of individual organelles and their regulations, it is critical to resolve the compositions of individual organelles, which relies on a rapid and efficient isolation method for specific organ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.235390
更新日期：2019-12-13 00:00:00
abstract：:Autophagy is a pivotal cytoprotective process that secures cellular homeostasis, fulfills essential roles in development, immunity and defence against pathogens, and determines the lifespan of eukaryotic organisms. However, autophagy also crucially contributes to the development of age-related human pathologies, inclu...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.146258
更新日期：2015-01-15 00:00:00
abstract：:Feedback mechanisms preventing M phase occurrence before S phase completion are assumed to depend on inhibition of cyclin B-cdc2 kinase activation by unreplicated DNA. In sea urchin, fertilization stimulates protein synthesis and releases eggs from G1 arrest. We found that in the one-cell sea urchin embryo cyclin B-cd...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:The translationally controlled protein P23 was discovered by the early induction of its rate of synthesis after mitogenic stimulation of mouse fibroblasts. P23 is expressed in almost all mammalian tissues and it is highly conserved between animals, plants and yeast. Based on its amino acid sequence, P23 cannot be attr...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
abstract：:TAR DNA-binding protein (TDP-43, also known as TARDBP) is the major pathological protein in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Large TDP-43 aggregates that are decorated with degradation adaptor proteins are seen in the cytoplasm of remaining neurons in ALS and FTD patients post mor...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.140087
更新日期：2014-03-15 00:00:00
abstract：:Pollen diffusates, known to be important in pollen-stigma interactions controlling interspecific incompatibility between Populus deltoides and Populus alba, have been partly characterized and shown to contain more than 20 protein bands by polyacrylamide gel electrophoresis, at least 4 of these being glycoproteins. Sev...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1980-08-01 00:00:00
abstract：:Cartilage oligomeric matrix protein (COMP) is an abundant component in the extracellular matrix (ECM) of load-bearing tissues such as tendons and cartilage. It provides adaptor functions by bridging different ECM structures. We have previously shown that COMP is also a constitutive component of healthy human skin and ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.180216
更新日期：2016-02-15 00:00:00
abstract：:We have isolated the human genes encoding the Pyst1 (MKP-3) and Pyst2 (MKP-X) MAP kinase phosphatases. Both genes consist of three exons interrupted by two introns and lack an intron which is conserved in all the other members of this gene family characterised to date. This reinforces the conclusion that Pyst1 and Pys...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：:MicroRNAs are known to play regulatory roles in gene expression associated with cancer development. We analyzed levels of the microRNA miR-24 in patients with breast carcinoma and found that miR-24 was higher in breast carcinoma samples than in benign breast tissues. We generated constructs expressing miR-24 and studi...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.118299
更新日期：2013-03-15 00:00:00
abstract：:The identification and characterization of scaffold and targeting proteins of the ERK/MAP kinase pathway is important to understand the function and intracellular organization of this pathway. The F-actin binding protein leukocyte-specific protein 1 (LSP1) binds to PKCbetaI and expression of B-LSP1, an LSP1 truncate c...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00955
更新日期：2004-04-15 00:00:00
abstract：:Serial sections of mitotic spindles of the marine cryptophycean alga, Cryptomonas, were analysed to determine what types of microtubules they contained and which of these microtubules came close enough to each other (50 nm or less) for the commonly described crossbridging to be possible. Interpolar microtubules were r...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-06-01 00:00:00
abstract：:Tau is a neuronal microtubule-associated protein that appears to function in the formation and maintenance of axons by influencing microtubule organisation. Tau is a phosphoprotein and is more heavily phosphorylated in fetal than in adult brain, and is also hyperphosphorylated in Alzheimer's disease where it forms the...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
abstract：:The vacuolar H(+)-ATPase (V-ATPase) is a multi-subunit enzyme that plays important roles in eukaryotic cells. In Dictyostelium, it is found primarily in membranes of the contractile vacuole complex, where it energizes fluid accumulation by this osmoregulatory organelle and also in membranes of endolysosomes, where it ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-07-15 00:00:00
abstract：:The interplay between pathogen-encoded virulence factors and host cell signaling networks is critical for both the establishment and clearance of microbial infections. Yersinia uptake into host cells serves as an in vitro model for exploring how host cells respond to Yersinia adherence. In this study, we provide insig...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-07-01 00:00:00
abstract：:Clathrin-mediated endocytosis requires cargo-specific adaptor proteins that recognize specific receptors and recruit them into coated pits. ARH [also called low-density lipoprotein receptor (LDLR) adaptor protein] serves as an adaptor for LDLR endocytosis in liver. However, ARH is dispensable for LDL uptake by some ot...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03217
更新日期：2006-10-15 00:00:00
abstract：:Astrin is a mitotic-spindle-associated protein expressed in most human cell lines and tissues. However, its functions in spindle organization and mitosis have not yet been determined. Sequence analysis revealed that astrin has an N-terminal globular domain and an extended coiled-coil domain. Recombinant astrin was pur...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00088
更新日期：2002-11-01 00:00:00
abstract：:Faithful genome transmission requires a network of pathways coordinating DNA replication to DNA repair and recombination. Here, we used molecular combing to measure the impact of homologous recombination (HR) on the velocity of DNA replication forks. We used three hamster cell lines defective in HR either by overexpre...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.010330
更新日期：2008-01-15 00:00:00
abstract：:Currently >50 proteins have been reported to be associated with focal contacts and related ECM adhesions. Most of these contain multiple domains through which they can interact with different molecular partners, potentially forming a dense and heterogeneous protein network at the cytoplasmic faces of the adhesion site...
journal_title：Journal of cell science
pub_type： 评论,杂志文章,评审
doi：
更新日期：2001-10-01 00:00:00
abstract：:Adaptive immunity, a vertebrate specialization, adds memory and exquisite specificity to the basic innate immune responses present in invertebrates while conserving metabolic resources. In adaptive immunity, antigenic challenge requires extremely rapid proliferation of rare antigen-specific lymphocytes to produce larg...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.096040
更新日期：2012-03-15 00:00:00
abstract：:Human breast cancer cell lines, as well as transformed mammary epithelial cells (HBL-100) and growth-stimulated normal breast epithelial cells showed positive cytochemical reaction with the proteinase substrate 2-(N-benzyloxycarbonyl-L-arginyl-L-arginylamido)-4-methoxynapht halene, in the presence of 5-nitrosalicylald...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1987-02-01 00:00:00
abstract：:Cells that are treated long-term with TNFalpha or short-term with TGFalpha together with cycloheximide (CHX) undergo apoptosis. Cell shrinkage and detachment during apoptosis is dependent on actomyosin contractility. Myosin II heavy chain (MHCII) isoforms have shared and distinct functions. Here, we investigated wheth...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.022640
更新日期：2008-05-15 00:00:00
abstract：:Integral membrane and secretory proteins which fail to fold productively are retained in the endoplasmic reticulum and targeted for degradation by cytoplasmic proteasomes. Genetic and biochemical analyses suggest that substrates of this pathway must be dislocated across the membrane of the endoplasmic reticulum (ER) b...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-07-15 00:00:00
abstract：:In mammals, fertilization initiates Ca2+ oscillations in metaphase II oocytes, which are required for the activation of embryo development. Germinal vesicle (GV) oocytes also display Ca2+ oscillations, although these unfold spontaneously in the absence of any known agonist(s) and their function remains unclear. We fou...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.225441
更新日期：2019-02-12 00:00:00
