Leukocyte-specific protein 1 targets the ERK/MAP kinase scaffold protein KSR and MEK1 and ERK2 to the actin cytoskeleton.

abstract：:Tissues fixed in osmium tetroxide or in combined osmium and glutaraldehyde (Hinde), embedded in Spurr's medium, cut at 0-5-I mum and mounted in Farrants' gum medium containing ethyl gallate, show good staining of lipid-contaning structures (droplets of triglyceride, membranes, mitochondria, etc.) in the light microsco...

journal_title：Journal of cell science

authors： Wigglesworth VB

更新日期：1975-12-01 00:00:00

abstract：:We analysed the phenotypic outcome of a Stra8-null mutation on male meiosis. Because the mutant spermatocytes (1) underwent premeiotic DNA replication, (2) displayed cytological features attesting initiation of recombination and of axial-element assembly, and (3) expressed Spo11 and numerous other meiotic genes, it wa...

journal_title：Journal of cell science

authors： Mark M,Jacobs H,Oulad-Abdelghani M,Dennefeld C,Féret B,Vernet N,Codreanu CA,Chambon P,Ghyselinck NB

更新日期：2008-10-01 00:00:00

abstract：:Endonuclease G is a mitochondrial protein implicated in DNA fragmentation during apoptosis in cell types ranging from fungi to mammals. Features of programmed cell death have been reported in a number of single-celled organisms, including the human trypanosomatid parasites Leishmania and Trypanosoma. However, the prot...

journal_title：Journal of cell science

authors： Gannavaram S,Vedvyas C,Debrabant A

更新日期：2008-01-01 00:00:00

abstract：:Epithelial-to-mesenchymal transition (EMT) of peritoneal mesothelial cells is a pathological process that occurs during peritoneal dialysis. EMT leads to peritoneal fibrosis, ultrafiltration failure and eventually to the discontinuation of therapy. Signaling pathways involved in mesothelial EMT are thus of great inter...

journal_title：Journal of cell science

authors： Strippoli R,Benedicto I,Foronda M,Perez-Lozano ML,Sánchez-Perales S,López-Cabrera M,Del Pozo MÁ

更新日期：2010-12-15 00:00:00

abstract：:Removal of the pro-domain of a cysteine protease is essential for activation of the enzyme. We have engineered a cysteine protease (CPB2.8) of the protozoan parasite Leishmania mexicana by site-directed mutagenesis to remove the active site cysteine (to produce CPB(C25G)). When CPB(C25G) was expressed in a L. mexicana...

journal_title：Journal of cell science

authors： Brooks DR,Tetley L,Coombs GH,Mottram JC

更新日期：2000-11-01 00:00:00

abstract：:Cells are internally organized into compartmentalized organelles that execute specialized functions. To understand the functions of individual organelles and their regulations, it is critical to resolve the compositions of individual organelles, which relies on a rapid and efficient isolation method for specific organ...

journal_title：Journal of cell science

authors： Xiong J,He J,Xie WP,Hinojosa E,Ambati CSR,Putluri N,Kim HE,Zhu MX,Du G

更新日期：2019-12-13 00:00:00

abstract：:The mAb RK7, previously shown to recognize keratin 19, was also found to cross-react with a biologically unrelated 102 kDa protein, which becomes associated with the poles of the mitotic apparatus. This newly identified protein, called cytocentrin, is a stable cellular component, may be at least in part phosphorylated...

journal_title：Journal of cell science

authors： Paul EC,Quaroni A

更新日期：1993-11-01 00:00:00

abstract：:Epithelial cell polarity is essential for animal development. The scaffold protein Bazooka (Baz/PAR-3) forms apical polarity landmarks to organize epithelial cells. However, it is unclear how Baz is recruited to the plasma membrane and how this is coupled with downstream effects. Baz contains an oligomerization domain...

journal_title：Journal of cell science

authors： McKinley RF,Yu CG,Harris TJ

更新日期：2012-03-01 00:00:00

abstract：:A myosin heavy chain polypeptide has been identified and localized in Nicotiana pollen tubes using monoclonal anti-myosin antibodies. The epitopes of these antibodies were found to reside on the myosin heavy chain head and rod portion and were, therefore, designated anti-S-1 (myosin S-1) and anti-LMM (light meromyosin...

journal_title：Journal of cell science

authors： Tang XJ,Hepler PK,Scordilis SP

更新日期：1989-04-01 00:00:00

abstract：:Low-intensity pulsed ultrasound (LIPUS) is a therapy used clinically to promote healing. Using live-cell imaging we show that LIPUS stimulation, acting through integrin-mediated cell-matrix adhesions, rapidly induces Rac1 activation associated with dramatic actin cytoskeleton rearrangements. Our study demonstrates tha...

journal_title：Journal of cell science

authors： Atherton P,Lausecker F,Harrison A,Ballestrem C

更新日期：2017-07-15 00:00:00

abstract：:New crystal structures of the kinesin motors differ from previously described motor-ADP atomic models, showing striking changes both in the switch I region near the nucleotide-binding cleft and in the switch II or 'relay' helix at the filament-binding face of the motor. The switch I region, present as a short helix fl...

journal_title：Journal of cell science

authors： Kull FJ,Endow SA

更新日期：2002-01-01 00:00:00

abstract：:Integrins play a key role in regulating endothelial cell survival, migration and differentiated function during angiogenic blood-vessel remodeling. Integrin-linked kinase (ILK) is a multidomain protein that interacts with the cytoplasmic tail of integrin beta subunits and is thought to participate in integrin-mediated...

journal_title：Journal of cell science

authors： Vouret-Craviari V,Boulter E,Grall D,Matthews C,Van Obberghen-Schilling E

更新日期：2004-09-01 00:00:00

abstract：:The ryanodine receptor (RyR) is an ion channel composed of four identical subunits mediating calcium efflux from the endo/sarcoplasmic reticulum of excitable and non-excitable cells. We present several lines of evidence indicating that the RyR2 N-terminus is capable of self-association. A combination of yeast two-hybr...

journal_title：Journal of cell science

authors： Zissimopoulos S,Viero C,Seidel M,Cumbes B,White J,Cheung I,Stewart R,Jeyakumar LH,Fleischer S,Mukherjee S,Thomas NL,Williams AJ,Lai FA

更新日期：2013-11-01 00:00:00

abstract：:The translationally controlled tumor protein (TCTP; also known as TPT1 in mammals) is highly conserved and ubiquitously expressed in eukaryotes. It is involved in growth and development, cell cycle progression, protection against cellular stresses and apoptosis, indicating the multifunctional role of the protein. Here...

journal_title：Journal of cell science

authors： Jojic B,Amodeo S,Bregy I,Ochsenreiter T

更新日期：2018-05-10 00:00:00

abstract：:Phagosomes are formed when phagocytic cells ingest particles such as bacteria, viruses or synthetic beads of different kinds. The environment within the phagosome gradually changes to generate degradative conditions. These changes require multiple interactions between the maturing phagosomes and the endocytic and the ...

journal_title：Journal of cell science

authors： Tjelle TE,Saigal B,Froystad M,Berg T

更新日期：1998-01-01 00:00:00

abstract：:Circadian rhythms exist in most living organisms. The general molecular mechanisms that are used to generate 24-hour rhythms are conserved among organisms, although the details vary. These core clocks consist of multiple regulatory feedback loops, and must be coordinated and orchestrated appropriately for the fine-tun...

journal_title：Journal of cell science

authors： Kojima S,Shingle DL,Green CB

更新日期：2011-02-01 00:00:00

abstract：:During mouse meiosis, the early prophase RAD51/DMC1 recombination protein sites, which are associated with the chromosome cores and which serve as markers for ongoing DNA-DNA interactions, are in ten-fold excess of the eventual reciprocal recombinant events. Most, if not all, of these early interactions are eliminated...

journal_title：Journal of cell science

authors： Moens PB,Kolas NK,Tarsounas M,Marcon E,Cohen PE,Spyropoulos B

更新日期：2002-04-15 00:00:00

abstract：:Both at a basal level and after induction (especially in response to nutrient starvation), the function of autophagy is to allow cells to degrade and recycle damaged organelles, proteins and other biological constituents. Here, we focus on the role microtubules have in autophagosome formation, autophagosome transport ...

journal_title：Journal of cell science

authors： Mackeh R,Perdiz D,Lorin S,Codogno P,Poüs C

更新日期：2013-03-01 00:00:00

abstract：:PINCH1 is composed of 5 LIM domains, binds integrin-linked kinase (ILK) and locates to integrin-mediated adhesion sites. In order to investigate PINCH1 function we generated mice and embryonic stem (ES) cell-derived embryoid bodies (EBs) lacking the PINCH1 gene. Similar to mice lacking beta1 integrin or Ilk, loss of P...

journal_title：Journal of cell science

authors： Li S,Bordoy R,Stanchi F,Moser M,Braun A,Kudlacek O,Wewer UM,Yurchenco PD,Fässler R

更新日期：2005-07-01 00:00:00

abstract：:Molecular mechanisms of chemotactic response are highly conserved among many eukaryotic cells including human leukocytes and Dictyostelium discoideum cells. The cells can sense the differences in chemoattractant concentration across the cell body and respond by extending pseudopods from the cell side facing to a highe...

journal_title：Journal of cell science

authors： Matsuoka S,Iijima M,Watanabe TM,Kuwayama H,Yanagida T,Devreotes PN,Ueda M

更新日期：2006-03-15 00:00:00

abstract：:The mammalian retromer complex is a multi-protein complex that regulates retrograde transport of the cation-independent mannose 6-phosphate receptor (CI-MPR) from early endosomes to the trans Golgi network (TGN). It consists of two subcomplexes: a membrane-bound coat comprising sorting nexin-1 (SNX1) and possibly sort...

journal_title：Journal of cell science

authors： Bujny MV,Popoff V,Johannes L,Cullen PJ

更新日期：2007-06-15 00:00:00

abstract：:We have used the H-7w subclone of a Chinese hamster ovary cell line (K1) to investigate the role of cell surface architecture (specifically microvilli, blebs, and sheets) in determining the relative agglutinability of a cell line with Con A. Our evidence clearly demonstrates that no specific, immediately recognizable ...

journal_title：Journal of cell science

authors： Noonan KD,Ukena T

更新日期：1978-12-01 00:00:00

abstract：:SNARE (Soluble N-ethyl-maleimide sensitive factor Attachment protein Receptor) proteins assemble in tight core complexes, which promote fusion of carrier vesicles with target compartments. Members of this class of proteins are expressed in all eukaryotic cells and are distributed in distinct subcellular compartments. ...

journal_title：Journal of cell science

authors： Rowe J,Calegari F,Taverna E,Longhi R,Rosa P

更新日期：2001-09-01 00:00:00

abstract：:Super-resolution fluorescence imaging by single-molecule photoactivation or photoswitching and position determination (localization microscopy) has the potential to fundamentally revolutionize our understanding of how cellular function is encoded at the molecular level. Among all powerful, high-resolution imaging tech...

journal_title：Journal of cell science

authors： Sauer M

更新日期：2013-08-15 00:00:00

abstract：:Centrioles organise centrosomes and cilia, and these organelles have an important role in many cell processes. In flies, the centriole protein Ana1 is required for the assembly of functional centrosomes and cilia. It has recently been shown that Cep135 (also known as Bld10) initially recruits Ana1 to newly formed cent...

journal_title：Journal of cell science

authors： Saurya S,Roque H,Novak ZA,Wainman A,Aydogan MG,Volanakis A,Sieber B,Pinto DM,Raff JW

更新日期：2016-07-01 00:00:00

abstract：:TRF1 is a key component of the telomere-capping complex and binds double-strand telomeric DNA as homodimers. So far, it is not clear whether TRF1 dimerization coincides with its telomere binding or is actively controlled before it binds the telomere, and in the latter case, how this event might affect its telomere ass...

journal_title：Journal of cell science

authors： Meng L,Hsu JK,Zhu Q,Lin T,Tsai RY

更新日期：2011-11-01 00:00:00

abstract：:Endothelial permeability induced by thrombin and histamine is accompanied by actin stress fibre assembly and intercellular gap formation. Here, we investigate the roles of the Rho family GTPases Rho1, Rac1 and Cdc42 in regulating endothelial barrier function, and correlate this with their effects on F-actin organizati...

journal_title：Journal of cell science

authors： Wójciak-Stothard B,Potempa S,Eichholtz T,Ridley AJ

更新日期：2001-04-01 00:00:00

abstract：:Elementary Ca(2+) signals, such as "Ca(2+) puffs", which arise from the activation of inositol 1,4,5-trisphosphate receptors, are building blocks for local and global Ca(2+) signalling. We characterized Ca(2+) puffs in six cell types that expressed differing ratios of the three inositol 1,4,5-trisphosphate receptor is...

journal_title：Journal of cell science

authors： Tovey SC,de Smet P,Lipp P,Thomas D,Young KW,Missiaen L,De Smedt H,Parys JB,Berridge MJ,Thuring J,Holmes A,Bootman MD

更新日期：2001-11-01 00:00:00

abstract：:p120-catenin is an E-cadherin-associated protein that modulates E-cadherin function and stability. We describe here that p120-catenin is required for Wnt pathway signaling. p120-catenin binds and is phosphorylated by CK1ε in response to Wnt3a. p120-catenin also associates to the Wnt co-receptor LRP5/6, an interaction ...

journal_title：Journal of cell science

authors： Casagolda D,Del Valle-Pérez B,Valls G,Lugilde E,Vinyoles M,Casado-Vela J,Solanas G,Batlle E,Reynolds AB,Casal JI,de Herreros AG,Duñach M

更新日期：2010-08-01 00:00:00

abstract：:A number of epithelia form tubulocysts in vitro when overlaid with type I collagen gel. Because collagen receptors are generally believed to be expressed on the basolateral domain, the mechanism by which collagen elicits this morphogenetic response from the apical surface is unclear. To investigate the role of beta 1 ...

journal_title：Journal of cell science

authors： Zuk A,Matlin KS

更新日期：1996-07-01 00:00:00