Abstract:
:Pollen diffusates, known to be important in pollen-stigma interactions controlling interspecific incompatibility between Populus deltoides and Populus alba, have been partly characterized and shown to contain more than 20 protein bands by polyacrylamide gel electrophoresis, at least 4 of these being glycoproteins. Seven fractions had antigenic activity in rabbits and several enzyme activities were also present. Peroxidase and leucine aminopeptidase isoenzymes were detected in the diffusates, demonstrating the extracellular location of these 2 enzymes. Isoenzyme patterns of peroxidase, esterase and acid phosphatase were complex, with some bands common to both species. Localization of acid phosphatase in the intine and esterase in the exine was demonstrated after brief aldehyde fixation and low-temperature embedding in glycol methacrylate. The intine and exine sites were distinguished by their chemical and structural features. Calcofluor white M2R new proved to be an excellent stain for differentiating the intine. Aniline blue-positive material, probably beta-1,3-glucan, is present associated with the intine of many ungerminated as well as germinating grains: production of this material may be a response to damage.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Ashford AE,Knox RBsubject
Has Abstractpub_date
1980-08-01 00:00:00pages
1-17eissn
0021-9533issn
1477-9137journal_volume
44pub_type
杂志文章abstract::In adherent cells, the relevance of a physical mechanotransduction pathway provided by the perinuclear actin cap stress fibers has recently emerged. Here, we investigate the impact of a functional actin cap on the cellular adaptive response to topographical cues and uniaxial cyclic strain. Lmna-deficient fibroblasts a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.184838
更新日期:2017-02-15 00:00:00
abstract::An electron-microscopic study of various fractions taken during a newly developed method for preparation of brush border vesicles from rat duodenum, has shown that vesiculation of microvilli can occur in situ on the brush border membrane. This mode of formation ensures that the vesicle membranes are oriented as in viv...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1981-02-01 00:00:00
abstract::Mutation of the LMNA gene, encoding nuclear lamin A and lamin C (hereafter lamin A/C), is a common cause of familial dilated cardiomyopathy (DCM). Among Finnish DCM patients, the founder mutation c.427T>C (p.S143P) is the most frequently reported genetic variant. Here, we show that p.S143P lamin A/C is more nucleoplas...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.184150
更新日期:2016-07-15 00:00:00
abstract::The presence of gap junctions in stratified epithelia has now been extensively documented, but there have been few attempts to quantify them. In the present report, samples of hamster cheek pouch mucosa were processed for electron microscopy and electron micrographs from defined basal, spinous and granular layers were...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00
abstract::Gap junctions (GJs) assembled from connexin (Cx) proteins allow direct cell-cell communication. While phosphorylation is known to regulate multiple GJ functions, much less is known about the role of ubiquitin in these processes. Using ubiquitylation-type-specific antibodies and Cx43 lysine-to-arginine mutants we show ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.204321
更新日期:2018-08-09 00:00:00
abstract::MIP and MP70 are putative gap junction components in the plasma membranes of the mammalian lens fibre cells. We show now that MP70 can be solubilized separately from MIP in mild detergent solutions, and that this treatment results in the dissociation of the fibre gap junctions. Solubilized MP70 was isolated as 16.9 S ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
abstract::Members of the PKC superfamily have been implicated in various migratory models and in particular in spatially restricted processes. However, defining the precise local events that underlie the PKC-dependent processes is constrained by the unspecific nature of interventions. Here we address this problem in relation to...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.062299
更新日期:2010-08-15 00:00:00
abstract::We have used MPM-2, a monoclonal antibody raised against mitotic HeLa cells, to stain a Drosophila cell line, whole mounts of Drosophila embryos, and sectioned tissue from embryonic and larval stages of development. MPM-2 recognizes a major phosphoprotein of approximately 125 X 10(3) Mr in Drosophila tissue culture ce...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1987-02-01 00:00:00
abstract::Inhibitory and excitatory neurons exhibit distinct patterns of development in the mammalian cerebral cortex. The morphological development of inhibitory and excitatory neurons derived from fetal rat cerebral cortex has now been compared in vitro. Inhibitory neurons were identified by immunofluorescence staining with a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00762
更新日期:2003-11-01 00:00:00
abstract::Earlier studies have established that the average speed of a replication fork is two to three times slower in early S-phase than in late S-phase and that the intracellular 2'-deoxyribonucleoside 5'-triphosphate pools grow during S-phase. In this study, the effect of the exogenous 2'-deoxyribonucleoside 5'-triphosphate...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-02-01 00:00:00
abstract::The cell types responsible for osteolysis associated with skeletal metastasis of solid malignancies are unclear. Tumour infiltrating macrophages (TIMs) isolated from primary mammary carcinomas of C3H/Avy mice were cultured on bone slices to assess their ability to resorb bone. After 14 days in co-culture with murine m...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-03-01 00:00:00
abstract::Experimentally introduced tension on kinetochores and their centromeres has been shown to stabilize kinetochore attachment to microtubules, modify kinetochore directional instability, and regulate cell-cycle progression into anaphase. In mitosis, kinetochore tension and the stretch of centromere chromatin are produced...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::The C terminus of the tubulin alpha-subunit of most eukaryotic cells undergoes a cycle of tyrosination and detyrosination using two specific enzymes, a tubulin tyrosine ligase (TTL) and a tubulin carboxypeptidase. Although this enzyme cycle is conserved in evolution and exhibits rapid turnover, the meaning of this mod...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Ezrin/radixin/moesin (ERM) proteins are thought to play an important role in organizing cortical actin-based cytoskeletons through cross-linkage of actin filaments with integral membrane proteins. Recent in vitro biochemical studies have revealed that ERM proteins phosphorylated on their COOH-terminal threonine residu...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::Maturation of nociceptive neurons depends on changes in transcription factors, ion channels and neuropeptides. Mature nociceptors initiate pain in part by drastically reducing the activation threshold via intracellular sensitization signaling. Whether sensitization signaling also changes during development and aging r...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.197327
更新日期:2017-07-01 00:00:00
abstract::Perilipin family proteins (Plins) coat the surface of intracellular neutral lipid storage droplets in various cell types. Studies across diverse species demonstrate that Plins regulate lipid storage metabolism through recruitment of lipases and other regulatory proteins to lipid droplet surfaces. Mammalian genomes hav...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.104943
更新日期:2012-09-01 00:00:00
abstract::Cells that are treated long-term with TNFalpha or short-term with TGFalpha together with cycloheximide (CHX) undergo apoptosis. Cell shrinkage and detachment during apoptosis is dependent on actomyosin contractility. Myosin II heavy chain (MHCII) isoforms have shared and distinct functions. Here, we investigated wheth...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.022640
更新日期:2008-05-15 00:00:00
abstract::The neuron specific Drosophila ELAV protein belongs to the ELAV family of RNA binding proteins which are characterized by three highly conserved RNA recognition motifs, an N-terminal domain, and a hinge region between the second and third RNA recognition motifs. Despite their highly conserved RNA recognition motifs th...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Yeast cells were cultivated at different growth rates in a chemostat by alterations in the flow of the limiting nutrient glucose and in batch cultures where variations in growth rate were achieved by alterations in the composition of nutrients. It was observed that the stage in the cycle at which S-phase was completed...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-06-01 00:00:00
abstract::Actin polymerization is a crucial process during sperm capacitation. We have recently described the participation of FAK during actin polymerization in guinea pig spermatozoa. However, the mechanism by which FAK mediates these processes is unknown. Our previous data have shown that MAPK1 (hereafter referred to as ERK2...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.239186
更新日期:2020-04-28 00:00:00
abstract::The invasiveness of mouse lymphocytes and thymocytes, rabbit peritoneal neutrophil granulocytes (PMNs), mouse peritoneal macrophages (both activated and non-activated) and pig endothelial cells was assayed by implanting these cells to the chick wing bud. Cells of each type moved into the wing mesenchyme, although acti...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-12-01 00:00:00
abstract::Cytokines, such as interferons, erythropoietin, leptin and most interleukins, signal through type 1 cytokine receptors and activate the canonical JAK-STAT pathway. Aberrant cytokine signalling underlies numerous pathologies and adequate, temporary receptor activation is therefore under tight control. Negative-feedback...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.078055
更新日期:2011-03-15 00:00:00
abstract::A laser light source along with a new method of preparing endosperm cells of Haemanthus katherinae for differential interference contrast (DIC) microscopy has led to increased visibility of kinetochore fibres. Little information is available concerning the behaviour of these fibres during anaphase in living cells. In ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::Mammalian cytochrome P-450s in the CYP1A gene family catalyse the oxidation of a wide range of drugs and foreign compounds resulting in their excretion. These enzymes are highly inducible by a range of compounds, including polycyclic aromatic hydrocarbons such as 3-methylcholanthrene (3-MC) and dioxins. Analysis of th...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract::Fatty acids constitute an important energy source for various tissues. The mechanisms that mediate and control uptake of free fatty acids from the circulation, however, are poorly understood. Here we show that efficient fatty-acid uptake by yeast cells requires the protein kinase Ypk1, the orthologue of the human seru...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.063073
更新日期:2010-07-01 00:00:00
abstract::Distinctive views of the tubulo-vesicular elements interposed between the endoplasmic reticulum (ER) and the Golgi apparatus were obtained in thin sections. The tubules that protrude from the transitional rough ER (tRER) are of dissimilar length. The numbers of tubules and of the nearby omega- and pear-shaped profiles...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::Low-density lipoprotein (LDL) deposition, aggregation and retention in the endothelial sub-intima are critical initiating events during atherosclerosis. Macrophages digest aggregated LDL (agLDL) through a process called exophagy. High-density lipoprotein (HDL) plays an atheroprotective role, but studies attempting to ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.237271
更新日期:2019-12-02 00:00:00
abstract::The process of angiogenesis requires endothelial cells (ECs) to undergo profound changes in shape and polarity. Although this must involve remodelling of the EC cytoskeleton, little is known about this process or the proteins that control it. We used a co-culture assay of angiogenesis to examine the cytoskeleton of EC...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.091066
更新日期:2012-03-15 00:00:00
abstract::Protein kinase CK2, a vital, pleiotropic and highly conserved serine/threonine phosphotransferase is involved in transcription-directed signaling, gene control and cell cycle regulation and is suspected to play a role in global processes. Searching for these global roles, we analyzed the involvement of CK2 in gene exp...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00352
更新日期:2003-04-15 00:00:00
abstract::The murine macrophage-like cell line P388D1 ingests immunoglobulin-coated sheep red cells (IgG-SRC) poorly, but after 3 days incubation in the presence of 1.5% dimethyl sulphoxide (DMSO), it becomes highly phagocytic. We used this model to correlate triggering of phagocytosis with some surface properties of P388D1 cel...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00