Abstract：
:Astrin is a mitotic-spindle-associated protein expressed in most human cell lines and tissues. However, its functions in spindle organization and mitosis have not yet been determined. Sequence analysis revealed that astrin has an N-terminal globular domain and an extended coiled-coil domain. Recombinant astrin was purified and characterized by CD spectroscopy and electron microscopy. Astrin showed parallel dimers with head-stalk structures reminiscent of motor proteins, although no sequence similarities to known motor proteins were found. In physiological buffers, astrin dimers oligomerized via their globular head domains and formed aster-like structures. Silencing of astrin in HeLa cells by RNA interference resulted in growth arrest, with formation of multipolar and highly disordered spindles. Chromosomes did not congress to the spindle equator and remained dispersed. Cells depleted of astrin were normal during interphase but were unable to progress through mitosis and finally ended in apoptotic cell death. Possible functions of astrin in mitotic spindle organization are discussed.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Gruber J,Harborth J,Schnabel J,Weber K,Hatzfeld Mdoi
10.1242/jcs.00088keywords:
subject
Has Abstractpub_date
2002-11-01 00:00:00pages
4053-9issue
Pt 21eissn
0021-9533issn
1477-9137journal_volume
115pub_type
杂志文章abstract：:We have studied caveolin-1 expression and the frequency and distribution of typical invaginated caveolae as they are identified by electron microscopy in the polarized epithelial cell lines MDCK II and Caco-2. In wild-type MDCK II cells caveolin expression is high and more than 400 caveolae/mm filter were observed at ...
journal_title：Journal of cell science
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更新日期：1998-03-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2003-10-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1975-05-01 00:00:00
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更新日期：2020-01-23 00:00:00
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更新日期：2015-03-15 00:00:00
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更新日期：2020-07-16 00:00:00
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journal_title：Journal of cell science
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更新日期：1989-02-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
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更新日期：2020-03-10 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2019-04-05 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.084152
更新日期：2012-06-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03217
更新日期：2006-10-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.036269
更新日期：2008-11-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
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更新日期：2004-01-15 00:00:00
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pub_type： 杂志文章
doi：
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pub_type： 杂志文章,评审
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更新日期：2018-10-04 00:00:00
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