Abstract:
:In S. cerevisiae, spindle orientation is linked to the inheritance of the ;old' spindle pole by the bud. A player in this asymmetric commitment, Bud6p, promotes cortical capture of astral microtubules. Additionally, Bud6p stimulates actin cable formation though the formin Bni1p. A relationship with the second formin, Bnr1p, is unclear. Another player is Kar9p, a protein that guides microtubules along actin cables organised by formins. Here, we ask whether formins mediate Bud6p-dependent microtubule capture beyond any links to Kar9p and actin. We found that both formins control Bud6p localisation. bni1 mutations advanced recruitment of Bud6p at the bud neck, ahead of spindle assembly, whereas bnr1Delta reduced Bud6p association with the bud neck. Accordingly, bni1 or bnr1 mutations redirected microtubule capture to or away from the bud neck, respectively. Furthermore, a Bni1p truncation that can form actin cables independently of Bud6p could not bypass a bud6Delta for microtubule capture. Conversely, Bud6(1-565)p, a truncation insufficient for correct actin organisation via formins, supported microtubule capture. Finally, Bud6p or Bud6(1-565)p associated with microtubules in vitro. Thus, surprisingly, Bud6p may promote microtubule capture independently of its links to actin organisation, whereas formins would contribute to the program of Bud6p-dependent microtubule-cortex interactions by controlling Bud6p localisation.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Delgehyr N,Lopes CS,Moir CA,Huisman SM,Segal Mdoi
10.1242/jcs.036269subject
Has Abstractpub_date
2008-11-15 00:00:00pages
3803-14issue
Pt 22eissn
0021-9533issn
1477-9137pii
jcs.036269journal_volume
121pub_type
杂志文章abstract::The 5T4 oncofoetal antigen is a 72 kDa glycoprotein defined by a monoclonal antibody raised against human placental trophoblast and is expressed in many different carcinomas but detected only at low levels in some normal epithelia. Immunohistochemical analysis of the patterns of expression in colorectal carcinomas has...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::CDC6, a replication licensing protein, is partially exported to the cytoplasm in human cells through phosphorylation by Cdk during S phase, but a significant proportion remains in the nucleus. We report here that human CDC6 physically interacts with ATR, a crucial checkpoint kinase, in a manner that is stimulated by p...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.058693
更新日期:2010-01-15 00:00:00
abstract::Although the anti-apoptotic activity of Bcl-2 has been extensively studied, its mode of action is still incompletely understood. In the nematode Caenorhabditis elegans, 131 of 1090 somatic cells undergo programmed cell death during development. Transgenic expression of human Bcl-2 reduced cell death during nematode de...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02985
更新日期:2006-06-15 00:00:00
abstract::The degradation of misfolded proteins is essential for cellular homeostasis. Misfolded proteins are normally degraded by the ubiquitin-proteasome system (UPS), and selective autophagy serves as a backup mechanism when the UPS is overloaded. Selective autophagy mediates the degradation of harmful material by its seques...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.214304
更新日期:2018-10-04 00:00:00
abstract::How ion channels localize and distribute on the cell membrane remains incompletely understood. We show that interventions that vary cell adhesion proteins and cell size also affect the membrane current density of inward-rectifier K+ channels (Kir2.1; encoded by KCNJ2) and profoundly alter the action potential shape of...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.225383
更新日期:2019-09-02 00:00:00
abstract::Phosphatidylinositol phosphates are important regulators of processes such as the cytoskeleton organization, membrane trafficking and gene transcription, which are all crucial for polarized cell growth. In particular, phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] has essential roles in polarized growth as well...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.122606
更新日期:2013-08-15 00:00:00
abstract::Faithful genome transmission requires a network of pathways coordinating DNA replication to DNA repair and recombination. Here, we used molecular combing to measure the impact of homologous recombination (HR) on the velocity of DNA replication forks. We used three hamster cell lines defective in HR either by overexpre...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.010330
更新日期:2008-01-15 00:00:00
abstract::Intercellular propagation of signals through connexin32-containing gap junctions is of major importance in physiological processes like nerve activity-dependent glucose mobilization in liver parenchymal cells and enzyme secretion from pancreatic acinar cells. In these cells, as in other organs, more than one type of c...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::The CCAAT/enhancer-binding protein beta (C/EBPbeta, also known as CEBPB) was first identified as a regulator of differentiation and inflammatory processes in adipose tissue and liver. Although C/EBPbeta was initially implicated in synaptic plasticity, its function in the brain remains largely unknown. We have previous...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.025031
更新日期:2008-04-15 00:00:00
abstract::Fine-tuning of NMDA glutamatergic receptor signalling strategically controls crucial brain functions. This process depends on several ligands and modulators, one of which unexpectedly includes the serine protease tissue-type plasminogen activator (tPA). In vitro, tPA increases NMDA-receptor-mediated calcium influx by ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03354
更新日期:2007-02-15 00:00:00
abstract::Low-density lipoprotein (LDL) deposition, aggregation and retention in the endothelial sub-intima are critical initiating events during atherosclerosis. Macrophages digest aggregated LDL (agLDL) through a process called exophagy. High-density lipoprotein (HDL) plays an atheroprotective role, but studies attempting to ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.237271
更新日期:2019-12-02 00:00:00
abstract::The signal recognition particle (SRP) is a ribonucleoprotein machine that controls the translation and intracellular sorting of membrane and secreted proteins. The SRP contains a core RNA subunit with which six proteins are assembled. Recent work in both yeast and mammalian cells has identified the nucleolus as a poss...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01726
更新日期:2005-03-15 00:00:00
abstract::Homologous to E6AP C-terminus (HECT) E3 ubiquitin ligases play a critical role in various cellular pathways, including but not limited to protein trafficking, subcellular localization, innate immune response, viral infections, DNA damage responses and apoptosis. To date, 28 HECT E3 ubiquitin ligases have been identifi...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.228072
更新日期:2020-04-07 00:00:00
abstract::Prions are proteins that access self-templating amyloid forms, which confer phenotypic changes that can spread from individual to individual within or between species. These infectious phenotypes can be beneficial, as with yeast prions, or deleterious, as with mammalian prions that transmit spongiform encephalopathies...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.051672
更新日期:2010-04-15 00:00:00
abstract::Unconventional myosin VIIA (Myo7a) is an actin-based motor molecule that normally functions in the cochlear hair cells of the inner ear. Mutations of MYO7A/Myo7a have been implicated in inherited deafness in both humans and mice. However, there is limited information about the functions of Myo7a outside of the special...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.209924
更新日期:2018-02-22 00:00:00
abstract::The DNA content of isolated micronuclei, differentiating macronuclei (macronuclear Anlagen), and adult macronuclei of Loxodes magnus was measured cytofluorimetrically in preparations stained with a Schiff-type reagent, auramine-SO2, following hydrochloric acid hydrolysis. The DNA content of the youngest macronuclear A...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Macroautophagy is a dynamic process whereby portions of the cytosol are encapsulated in double-membrane vesicles and delivered to the lysosome for degradation. Phosphatidylinositol-3-phosphate (PtdIns3P) is concentrated on autophagic vesicles and recruits effector proteins that are crucial for this process. The produc...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.080341
更新日期:2011-03-01 00:00:00
abstract::Two variants (74AD and 74FL) established from rat ascites hepatoma AH7974 were examined for the production of glycosaminoglycans in culture. There was no difference between the adhesive (74AD) and the floating (74FL) variants in quantity of glycosaminoglycans produced by their cultivation in minimum essential medium s...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
abstract::The locomotion of single epidermis cells, grown out from Xenopus laevis tadpole tails has been investigated by time-lapse cinemicrography using phase-contrast and reflection-contrast optics. The cells develop a large, mostly 200-250 nm thick, lamella, which adheres homogeneously to the supporting coverglass and exceed...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1981-12-01 00:00:00
abstract::The interaction of the non-receptor tyrosine kinase, Src, with the cytoskeleton of adhesion sites was studied in nerve growth cones isolated from fetal rat brain. Of particular interest was the role of protein tyrosine phosphatases in the regulation of Src-cytoskeleton binding. Growth cones were found to contain a hig...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract::In adherent cells, the relevance of a physical mechanotransduction pathway provided by the perinuclear actin cap stress fibers has recently emerged. Here, we investigate the impact of a functional actin cap on the cellular adaptive response to topographical cues and uniaxial cyclic strain. Lmna-deficient fibroblasts a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.184838
更新日期:2017-02-15 00:00:00
abstract::Drosophila abnormal spindle (asp) mutants exhibit a mitotic metaphase checkpoint arrest with abnormal spindle poles, which reflects a requirement for Asp for the integrity of microtubule organising centres (MTOCs). In male meiosis, the absence of a strong spindle integrity checkpoint enables asp mutant cells to procee...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-03-01 00:00:00
abstract::Clusterin expression is increased in tissues undergoing apoptosis, including neurodegenerative retina, but the causal relationships remain to be clarified. To test the hypothesis that overexpression of clusterin could induce apoptosis in neurons, transgenic mice were generated in which rat clusterin transgene was expr...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::In many physiological contexts, intracellular reduction-oxidation (redox) conditions and the unfolded protein response (UPR) are important for the control of cell life and death decisions. UPR is triggered by the disruption of endoplasmic reticulum (ER) homeostasis, also known as ER stress. Depending on the duration a...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.153643
更新日期:2014-09-01 00:00:00
abstract::Alpha4 integrins are used by leukocytes and neural crest derivatives for adhesion and migration during embryogenesis, immune responses and tumour invasion. The pro-migratory activity of alpha4 integrin is mediated in part through the direct binding of the cytoplasmic domain to paxillin. Here, using intermolecular FRET...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.049130
更新日期:2009-05-15 00:00:00
abstract::The conversion of the cellular prion protein (PrPc) into a pathogenic isoform (PrP(Sc)) is one of the underlying events in the pathogenesis of the fatal transmissible spongiform encephalopathies (TSEs). Numerous compounds have been described to inhibit prion replication and PrP(Sc) accumulation in cell culture. Among ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02609
更新日期:2005-11-01 00:00:00
abstract::The formation and extension of filopodia in response to an extracellular stimulus by guidance cues determine the path of growth cone advance. Actin-filament bundling and actin polymerization at the tips supply the driving force behind the formation and elongation. We tried to clarify how signals in response to extrace...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00233
更新日期:2003-01-15 00:00:00
abstract::Phagocytosis is an actin-based process used by macrophages to clear particles greater than 0.5 microm in diameter. In addition to its role in immunological responses, phagocytosis is also necessary for tissue remodeling and repair. To prevent catastrophic autoimmune reactions, phagocytosis must be tightly regulated. I...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-02-15 00:00:00
abstract::Drosophila neural stem cells or neuroblasts undergo typical asymmetric cell division. An evolutionally conserved protein complex, comprising atypical protein kinase C (aPKC), Bazooka (Par-3) and Par-6, organizes cell polarity to direct these asymmetric divisions. Aurora-A (AurA) is a key molecule that links the divisi...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.050955
更新日期:2009-09-15 00:00:00
abstract::During meiotic prophase in the ciliate Tetrahymena thermophila micronuclei dramatically elongate and form thread-like crescents. The arrangement of the chromosomes within the crescent as well as the timing of chromosome pairing and recombination with respect to the elongation process have been subjects of ongoing deba...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01504
更新日期:2004-11-15 00:00:00