Abstract：
:Although the anti-apoptotic activity of Bcl-2 has been extensively studied, its mode of action is still incompletely understood. In the nematode Caenorhabditis elegans, 131 of 1090 somatic cells undergo programmed cell death during development. Transgenic expression of human Bcl-2 reduced cell death during nematode development, and partially complemented mutation of ced-9, indicating that Bcl-2 can functionally interact with the nematode cell death machinery. Identification of the nematode target(s) of Bcl-2 inhibition would help clarify the mechanism by which Bcl-2 suppresses apoptosis in mammalian cells. Exploiting yeast-based systems and biochemical assays, we analysed the ability of Bcl-2 to interact with and regulate the activity of nematode apoptosis proteins. Unlike CED-9, Bcl-2 could not directly associate with the caspase-activating adaptor protein CED-4, nor could it inhibit CED-4-dependent yeast death. By contrast, Bcl-2 could bind the C. elegans pro-apoptotic BH3-only Bcl-2 family member EGL-1. These data prompt us to hypothesise that Bcl-2 might suppress nematode cell death by preventing EGL-1 from antagonising CED-9, rather than by inhibiting CED-4.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Jabbour AM,Puryer MA,Yu JY,Lithgow T,Riffkin CD,Ashley DM,Vaux DL,Ekert PG,Hawkins CJdoi
10.1242/jcs.02985subject
Has Abstractpub_date
2006-06-15 00:00:00pages
2572-82issue
Pt 12eissn
0021-9533issn
1477-9137pii
jcs.02985journal_volume
119pub_type
杂志文章abstract：:Studies of human cell hybrids have provided evidence that the tumorigenicity of a cervical carcinoma (HeLa) is under the control of a putative tumor suppressor on chromosome 11. Using these human cell hybrids, we found a tumor-associated glycosylation change in the glucose transporter GLUT1, which is an N-linked glyco...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02314
更新日期：2005-05-01 00:00:00
abstract：:Vaccinia virus B1 kinase plays a key role in viral DNA replication. The homologous mammalian vaccinia-related kinases (VRKs) are also implicated in the regulation of DNA replication, although direct evidence remains elusive. Here we show that VRK1 regulates cell cycle progression in the DNA replication period by induc...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.026757
更新日期：2008-09-15 00:00:00
abstract：:Cellular activities are regulated by environmental stimuli through protein phosphorylation. Target of rapamycin (TOR), a serine/threonine kinase, plays pivotal roles in cell proliferation and cell growth in response to nutrient status. In Schizosaccharomyces pombe, TORC1, which contains Tor2, plays crucial roles in nu...
journal_title：Journal of cell science
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doi：10.1242/jcs.060319
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：
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pub_type： 评论,杂志文章,评审
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journal_title：Journal of cell science
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doi：10.1242/jcs.02380
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journal_title：Journal of cell science
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doi：
更新日期：1996-12-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1997-06-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1987-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1987-02-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1982-12-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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更新日期：1977-01-01 00:00:00
