The distribution of a 'mitosis-specific' antigen during Drosophila development.

abstract：:The SPN antigen plays an essential role in mitosis, since microinjection of antibodies causes mitotic arrest. Here we show, by examination of the relative locations of SPN antigen, the centrosomal 5051 antigen and tubulin in normal mitotic, and in taxol-treated mitotic cells, that the SPN antigen is involved in organi...

journal_title：Journal of cell science

authors： Kallajoki M,Weber K,Osborn M

更新日期：1992-05-01 00:00:00

abstract：:Oocytes of Xenopus laevis in pachytene and early diplotene of meiosis have been studied using the Miller spreading technique. Transcription first appears in germinal vesicles 25-40 micrometers in diameter, when the oocyte is in early diplotene. Transcription at this stage consists of arrays of short RNP transcripts, i...

journal_title：Journal of cell science

authors： Hill RS,Macgregor HC

更新日期：1980-08-01 00:00:00

abstract：:The adaptor protein SETA/CIN85/Ruk is involved in regulating diverse signal transduction pathways, including the internalization of tyrosine kinase receptors via the Cbl ubiquitin ligases, and attenuating PI3K activity by interaction with its regulatory subunit. Here we present evidence for a new aspect of SETA functi...

journal_title：Journal of cell science

authors： Schmidt MHH,Chen B,Randazzo LM,Bogler O

更新日期：2003-07-15 00:00:00

abstract：:Cytoplasmic dynein-2 is a motor protein complex that drives the movement of cargoes along microtubules within cilia, facilitating the assembly of these organelles on the surface of nearly all mammalian cells. Dynein-2 is crucial for ciliary function, as evidenced by deleterious mutations in patients with skeletal abno...

journal_title：Journal of cell science

authors： Vuolo L,Stevenson NL,Mukhopadhyay AG,Roberts AJ,Stephens DJ

更新日期：2020-03-30 00:00:00

abstract：:Glomerular mesangial cells (MCs) are central to the pathogenesis of progressive glomeruli-associated renal diseases. However, molecular mechanisms underlying changes in MC functions still remain poorly understood. Here, we show that in MCs, the urokinase-type plasminogen activator (uPA) induces, via its specific recep...

journal_title：Journal of cell science

authors： Shushakova N,Tkachuk N,Dangers M,Tkachuk S,Park JK,Hashimoto K,Haller H,Dumler I

更新日期：2005-06-15 00:00:00

abstract：:In this paper, we identify the chondroitin sulfate proteoglycan versican V2 as a major inhibitor of axonal growth in the extracellular matrix of the mature central nervous system. In immunohistochemical and in situ hybridization experiments we show that this tissue-specific splice variant of versican is predominantly ...

journal_title：Journal of cell science

authors： Schmalfeldt M,Bandtlow CE,Dours-Zimmermann MT,Winterhalter KH,Zimmermann DR

更新日期：2000-03-01 00:00:00

abstract：:Actins are major eukaryotic cytoskeletal proteins, and they are involved in many important cell functions, including cell division, cell polarity, wound healing and muscle contraction. Despite obvious drawbacks, muscle actin, which is easily purified, is used extensively for biochemical studies of the non-muscle actin...

journal_title：Journal of cell science

authors： Hatano T,Alioto S,Roscioli E,Palani S,Clarke ST,Kamnev A,Hernandez-Fernaud JR,Sivashanmugam L,Chapa-Y-Lazo B,Jones AME,Robinson RC,Sampath K,Mishima M,McAinsh AD,Goode BL,Balasubramanian MK

更新日期：2018-04-23 00:00:00

abstract：:Epitectin, the mucin-like glycoprotein defined by the monoclonal antibodies CA1, CA2 and CA3, has been examined by electron microscopy to determine its shape and size. It appears to be a single extended strand with a mean length of about 270 nm. The antibodies CA1 and CA2 appear to bind preferentially to a terminal si...

journal_title：Journal of cell science

authors： Bramwell ME,Wiseman G,Shotton DM

更新日期：1986-12-01 00:00:00

abstract：:By an improved freeze-fracture device, a number of double replicas were obtained from the rod disk membranes of the carp retina for investigation of complementary, continuing, and transitional relationships between fracture faces. Some separate particles found along the smooth edge bordering on the adjacent interdisk ...

journal_title：Journal of cell science

authors： Tonosaki A,Washioka H,Yamasaki M,Mizoguchi J

更新日期：1980-04-01 00:00:00

abstract：:Formation of crossovers between homologous chromosomes during meiosis is positively regulated by the ZMM proteins (also known as SIC proteins). DNA damage checkpoint proteins also promote efficient formation of interhomolog crossovers. Here, we examined, in budding yeast, the meiotic role of the heterotrimeric DNA dam...

journal_title：Journal of cell science

authors： Shinohara M,Hayashihara K,Grubb JT,Bishop DK,Shinohara A

更新日期：2015-04-15 00:00:00

abstract：:The tetrapeptide N-Acetyl-Seryl-Aspartyl-Lysyl-Proline (AcSDKP) is a natural regulator of hematopoietic stem cell proliferation. The present study was aimed at investigating the presence and the role of AcSDKP in rat testis. Specific immunoreactivity was always observed in the interstitial tissue at all stages of test...

journal_title：Journal of cell science

authors： Stéphan J,Melaine N,Ezan E,Hakovirta H,Maddocks S,Toppari J,Garnier D,Wdzieczak-Bakala J,Jégou B

更新日期：2000-01-01 00:00:00

abstract：:In order to study the dynamic behavior of intermediate filament networks in living cells, we have prepared constructs fusing green fluorescent protein to intermediate filament proteins. Vimentin fused to green fluorescent protein labeled the endogenous intermediate filament network. We generated stable SW13 and NIH3T3...

journal_title：Journal of cell science

authors： Ho CL,Martys JL,Mikhailov A,Gundersen GG,Liem RK

更新日期：1998-07-01 00:00:00

abstract：:The p53 tumor suppressor protein is a key regulator of cellular functions including responses to numerous stress signals, and triggers apoptosis in many cell types, including neurons. The major mechanisms known to regulate p53 stabilization and activation include phosphorylation and ubiquitin ligase-mediated proteasom...

journal_title：Journal of cell science

authors： Lee JH,Kim HS,Lee SJ,Kim KT

更新日期：2007-07-01 00:00:00

abstract：:Collagen binding proteins (CBP) are hydrophobic, cell surface polypeptides, isolated by collagen affinity chromatography. Antibodies to CBPs inhibit the attachment of embryonic chicken heart fibroblasts to native type I collagen fibrils in a dose-dependent manner. The CBP antibodies also induce rounding and detachment...

journal_title：Journal of cell science

authors： Ogle RC,Potts AJ,Yacoe M,Little CD

更新日期：1989-10-01 00:00:00

abstract：:The E-cadherin-catenin complex regulates Ca(2+)-dependent cell-cell adhesion and is localized to the basolateral membrane of polarized epithelial cells. Uncoupling beta-catenin from E-cadherin by deletion or substitution mutations causes accumulation of these proteins in intracellular compartments, including the trans...

journal_title：Journal of cell science

authors： Miyashita Y,Ozawa M

更新日期：2007-12-15 00:00:00

abstract：:Dispersion of neural crest cells and their ultimate regroupment into peripheral ganglia are associated with precisely coordinated regulations both in time and space of the expression and function of cell adhesion receptors. In particular, the disappearance of N-cadherin from the cell surface at the onset of migration ...

journal_title：Journal of cell science

authors： Monier-Gavelle F,Duband JL

更新日期：1995-12-01 00:00:00

abstract：:Increasing evidence indicates that cellular uptake of several molecules can occur independently of functional dynamin, but the molecular players that regulate dynamin-independent endocytosis and the subsequent trafficking steps are still largely unknown. A survival-based short-hairpin (sh) RNA screen using a cell line...

journal_title：Journal of cell science

authors： Krag C,Malmberg EK,Salcini AE

更新日期：2010-12-15 00:00:00

abstract：:In Saccharomyces cerevisiae, DNA replication stress activates the replication checkpoint, which slows S-phase progression, stabilizes slowed or stalled replication forks, and relieves inhibition of the ribonucleotide reductase (RNR) complex. To identify novel genes that promote cellular viability after replication str...

journal_title：Journal of cell science

authors： Woolstencroft RN,Beilharz TH,Cook MA,Preiss T,Durocher D,Tyers M

更新日期：2006-12-15 00:00:00

abstract：:Plectin, a cytolinker protein greater than 500 kDa in size, has an important role as a mechanical stabiliser of cells. It interlinks the various cytoskeletal filament systems and anchors intermediate filaments to peripheral junctional complexes. In addition, there is increasing evidence that plectin acts as a scaffold...

journal_title：Journal of cell science

authors： Gregor M,Zeöld A,Oehler S,Marobela KA,Fuchs P,Weigel G,Hardie DG,Wiche G

更新日期：2006-05-01 00:00:00

abstract：:MACF1 (microtubule actin crosslinking factor), also called ACF7 (actin crosslinking family 7) is a cytoskeletal linker protein that can associate with both actin filaments and microtubules. We have identified a novel alternatively spliced isoform of MACF1. We named this isoform MACF1b and renamed the original isoform ...

journal_title：Journal of cell science

authors： Lin CM,Chen HJ,Leung CL,Parry DA,Liem RK

更新日期：2005-08-15 00:00:00

abstract：:The protein import machinery of the peroxisome consists of many proteins, collectively called the peroxins. By applying the split-ubiquitin technique we systematically tested the pair-wise interactions between the Nub- and Cub-labeled peroxins for the first time in the living cells of the yeast Saccharomyces cerevisia...

journal_title：Journal of cell science

authors： Eckert JH,Johnsson N

更新日期：2003-09-01 00:00:00

abstract：:The CHO1 antigen is a mitosis-specific kinesin-like motor located at the interzonal region of the spindle. The human cDNA coding for the antigen contains a domain with sequence similarity to the motor domain of kinesin-like protein (Nislow et al., Nature 359, 543, 1992). Here we cloned cDNAs encoding the CHO1 antigen ...

journal_title：Journal of cell science

authors： Kuriyama R,Dragas-Granoic S,Maekawa T,Vassilev A,Khodjakov A,Kobayashi H

更新日期：1994-12-01 00:00:00

abstract：:This study focuses on the effect of static and dynamic mechanical compression on the biosynthetic activity of chondrocytes cultured within agarose gel. Chondrocyte/agarose disks (3 mm diameter) were placed between impermeable platens and subjected to uniaxial unconfined compression at various times in culture (2-43 da...

journal_title：Journal of cell science

authors： Buschmann MD,Gluzband YA,Grodzinsky AJ,Hunziker EB

更新日期：1995-04-01 00:00:00

abstract：:Regulated cell shape changes in epithelial cells, which contribute to most organs and tissues, are at the basis of morphogenesis. Crumbs (Crb) is an essential apical determinant controlling epithelial apicobasal polarity. Here we provide evidence for a novel role of Crb apical localisation and stabilisation in control...

journal_title：Journal of cell science

authors： Letizia A,Ricardo S,Moussian B,Martín N,Llimargas M

更新日期：2013-05-15 00:00:00

abstract：:Disabled proteins are a conserved family of monomeric adaptor proteins that in mammals are implicated in the endocytosis of lipoprotein receptors. Previous studies have shown that the sole Caenorhabditis elegans Disabled homologue, DAB-1, is involved in the lipoprotein receptor-mediated secretion of a fibroblast growt...

journal_title：Journal of cell science

authors： Holmes A,Flett A,Coudreuse D,Korswagen HC,Pettitt J

更新日期：2007-08-01 00:00:00

abstract：:Wound healing crucially relies on the mechanical activity of fibroblasts responding to TGFβ1 and to forces transmitted across focal adhesions. Integrin-linked kinase (ILK) is a central adapter recruited to integrin β1 tails in focal adhesions mediating the communication between cells and extracellular matrix. Here, we...

journal_title：Journal of cell science

authors： Blumbach K,Zweers MC,Brunner G,Peters AS,Schmitz M,Schulz JN,Schild A,Denton CP,Sakai T,Fässler R,Krieg T,Eckes B

更新日期：2010-11-15 00:00:00

abstract：:SARA, an early endosomal protein, plays a key role in TGFβ signalling, as it presents SMAD2 and SMAD3 for phosphorylation by the activated TGFβ receptors. Here, we show that ERBIN is a new SARA-interacting protein that can be recruited by SARA to early endosomes. ERBIN was recently shown to bind and segregate phosphor...

journal_title：Journal of cell science

authors： Sflomos G,Kostaras E,Panopoulou E,Pappas N,Kyrkou A,Politou AS,Fotsis T,Murphy C

更新日期：2011-10-01 00:00:00

abstract：:Axin and conductin (also known as axin2) are structurally related inhibitors of Wnt/β-catenin signalling that promote degradation of β-catenin. Whereas axin is constitutively expressed, conductin is a Wnt target gene implicated in Wnt negative-feedback regulation. Here, we show that axin and conductin differ in their ...

journal_title：Journal of cell science

authors： Bernkopf DB,Hadjihannas MV,Behrens J

更新日期：2015-01-01 00:00:00

abstract：:Separation of clathrin-coated pits from the plasma membrane, a key event during endocytosis, is thought to be driven by dynamin and the actin cytoskeleton. However, the mechanism for the actin-mediated endocytosis remains elusive. RNA interference-mediated suppression of cortactin, an F-actin binding protein that prom...

journal_title：Journal of cell science

authors： Zhu J,Zhou K,Hao JJ,Liu J,Smith N,Zhan X

更新日期：2005-02-15 00:00:00

abstract：:Phagocytosis is an actin-based process used by macrophages to clear particles greater than 0.5 microm in diameter. In addition to its role in immunological responses, phagocytosis is also necessary for tissue remodeling and repair. To prevent catastrophic autoimmune reactions, phagocytosis must be tightly regulated. I...

journal_title：Journal of cell science

authors： Beningo KA,Wang YL

更新日期：2002-02-15 00:00:00