Abstract:
:Plectin, a cytolinker protein greater than 500 kDa in size, has an important role as a mechanical stabiliser of cells. It interlinks the various cytoskeletal filament systems and anchors intermediate filaments to peripheral junctional complexes. In addition, there is increasing evidence that plectin acts as a scaffolding platform that controls the spatial and temporal localisation and interaction of signaling proteins. In this study we show that, in differentiated mouse myotubes, plectin binds to the regulatory gamma1 subunit of AMP-activated protein kinase (AMPK), the key regulatory enzyme of energy homeostasis. No interaction was observed in undifferentiated myoblasts, and plectin-deficient myotubes showed altered positioning of gamma1-AMPK. In addition we found that plectin affects the subunit composition of AMPK, because isoform alpha1 of the catalytic subunit decreased in proportion to isoform alpha2 during in vitro differentiation of plectin(-/-) myotubes. In plectin-deficient myocytes we could also detect a higher level of activated (Thr172-phosphorylated) AMPK, compared with wild-type cells. Our data suggest a differentiation-dependent association of plectin with AMPK, where plectin selectively stabilises alpha1-gamma1 AMPK complexes by binding to the gamma1 regulatory subunit. The distinct plectin expression patterns in different fibre types combined with its involvement in the regulation of isoform compositions of AMPK complexes could provide a mechanism whereby cytoarchitecture influences energy homeostasis.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Gregor M,Zeöld A,Oehler S,Marobela KA,Fuchs P,Weigel G,Hardie DG,Wiche Gdoi
10.1242/jcs.02891subject
Has Abstractpub_date
2006-05-01 00:00:00pages
1864-75issue
Pt 9eissn
0021-9533issn
1477-9137pii
jcs.02891journal_volume
119pub_type
杂志文章abstract::Xenopus embryos initiate replication at random closely spaced sites until a certain concentration of nuclei is achieved within the embryo, after which fewer, more specific chromosomal sites are utilized as origins. We have examined the relationship between nucleo-cytosolic ratio and origin specification when Chinese h...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract::While the basic cellular contributions to bone differentiation and mineralization are widely accepted, the regulation of these processes at the intracellular level remains inadequately understood. Our laboratory recently identified annexin 2 as a protein involved in osteoblastic mineralization. Annexin 2 was overexpre...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00909
更新日期:2004-01-26 00:00:00
abstract::Cell migration is required during development of the nervous system. The regulatory mechanisms for this process, however, are poorly elucidated. We show here that expression of or exposure to the neural cell adhesion molecule (NCAM) strongly affected the motile behaviour of glioma cells independently of homophilic NCA...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-01-15 00:00:00
abstract::Mitochondrial homeostasis reflects a dynamic balance between membrane fission and fusion events thought essential for mitochondrial function. We report here that altered expression of the C. elegans BCL2 homolog CED-9 affects both mitochondrial fission and fusion. Although striated muscle cells lacking CED-9 have no a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.032904
更新日期:2008-10-15 00:00:00
abstract::The postnatal mouse ovary is rich in quiescent and early-growing oocytes, each one surrounded by a layer of somatic granulosa cells (GCs) on a basal lamina. As oocytes start to grow the GCs change shape from flattened to cuboidal, increase their proliferation and form multiple layers, providing a unique model for stud...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.036400
更新日期:2008-12-01 00:00:00
abstract::Ubiquitinated membrane proteins such as epidermal growth factor receptor (EGFR) are delivered to early endosomes and then sorted to lysosomes via multivesicular bodies (MVBs) for degradation. The regulatory mechanism underlying formation of intralumenal vesicles en route to generation of MVBs is not fully understood. ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.229179
更新日期:2019-10-03 00:00:00
abstract::Targeting of glycosylphosphatidylinositol-anchored proteins to the apical surface of epithelial cells involves clustering in Triton X-100-resistant membrane microdomains or rafts. The role of these microdomains in sorting transmembrane proteins is more questionable because, unlike glycosylphosphatidylinositol-anchored...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.002717
更新日期:2007-03-15 00:00:00
abstract::Phagocytosis is an actin-based process used by macrophages to clear particles greater than 0.5 microm in diameter. In addition to its role in immunological responses, phagocytosis is also necessary for tissue remodeling and repair. To prevent catastrophic autoimmune reactions, phagocytosis must be tightly regulated. I...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-02-15 00:00:00
abstract::Eukaryotic cells replicate and partition their organelles between the mother cell and the daughter cell at cytokinesis. Polarized cells, notably the budding yeast Saccharomyces cerevisiae, are well suited for the study of organelle inheritance, as they facilitate an experimental dissection of organelle transport and r...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.151423
更新日期:2015-02-15 00:00:00
abstract::Mitotic progression is timely regulated by the accumulation and degradation of A- and B-type cyclins. In plants, there are three classes of A-, and two classes of B-type cyclins, but their specific roles are not known. We have generated transgenic tobacco plants in which the ectopic expression of a plant cyclin B2 gen...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00250
更新日期:2003-02-01 00:00:00
abstract::The surface coat of procyclic forms of Trypanosoma brucei consists of related, internally repetitive glycoproteins known as EP and GPEET procyclins. Previously we showed that the extracellular domain of GPEET is phosphorylated. We now show that phosphorylation of this glycosylphosphatidylinositol-anchored surface prot...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::Clathrin heavy chain is the structural component of the clathrin triskelion, but unique functions for the two distinct and highly conserved clathrin light chains (CLCa and CLCb, also known as CLTA and CLTB, respectively) have been elusive. Here, we show that following detachment and replating, CLCa is uniquely respons...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.224030
更新日期:2019-05-15 00:00:00
abstract::IGFBP3 is a multi-functional protein that has IGF-dependent and IGF-independent actions in cultured cells. Here we show that the IGF binding domain (IBD), nuclear localization signal (NLS) and transactivation domain (TA) are conserved and functional in zebrafish Igfbp3. The in vivo roles of these domains were investig...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.082644
更新日期:2011-06-01 00:00:00
abstract::During their development, mammalian oocytes acquire the ability to resume meiosis. We demonstrate that the concentration of p34cdc2 increases during the acquisition of meiotic competence, as determined by immunoblotting, whereas the concentration of cyclin B1 decreases. Laser-scanning confocal microscopy corroborated ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract::alphaA-Crystallin (alphaA) is a molecular chaperone expressed preferentially in the lens. alphaA transcripts are first detected during the early stages of lens development and its synthesis continues as the lens grows throughout life. alphaA(-/-) mouse lenses are smaller than controls, and lens epithelial cells derive...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00325
更新日期:2003-03-15 00:00:00
abstract::Recent studies show that mitochondria and actin filaments work together in two contexts: (1) increased cytoplasmic calcium induces cytoplasmic actin polymerization that stimulates mitochondrial fission and (2) mitochondrial depolarization causes actin assembly around mitochondria, with roles in mitophagy. It is unclea...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.234435
更新日期:2019-09-23 00:00:00
abstract::We uncovered a crucial role for the Aurora kinase A (AURKA)-Twist1 axis in promoting epithelial-to-mesenchymal transition (EMT) and chemoresistance in pancreatic cancer. Twist1 is the first EMT-specific target of AURKA that was identified using an innovative screen. AURKA phosphorylates Twist1 at three sites, which re...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.196790
更新日期:2017-03-15 00:00:00
abstract::Separation of clathrin-coated pits from the plasma membrane, a key event during endocytosis, is thought to be driven by dynamin and the actin cytoskeleton. However, the mechanism for the actin-mediated endocytosis remains elusive. RNA interference-mediated suppression of cortactin, an F-actin binding protein that prom...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01668
更新日期:2005-02-15 00:00:00
abstract::According to the hypothesis of contact inhibition of movement, cells in a confluent monolayer are restrained from major overlapping by a directional inhibition of locomotion. This explanation of monolayering proposes that contact between 2 cells locally paralyses the locomotory function, preventing movement in the dir...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-04-01 00:00:00
abstract::Male infertility has become a worldwide health problem, but the etiologies of most cases are still unknown. SEPT12, a GTP-binding protein, is involved in male fertility. Two SEPT12 mutations (SEPT12(T89M) and SEPT12(D197N)) have been identified in infertile men who have a defective sperm annulus with a bent tail. The ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.158998
更新日期:2015-03-01 00:00:00
abstract::gamma-Tubulin exists in fission yeast as the product of an essential gene, encoding a 446 amino acid protein that is 77.3% identical to Aspergillus nidulans gamma-tubulin. The gene disruption caused cell lethality, displaying condensed, undivided chromosomes with aberrant spindle structures. Anti-gamma-tubulin stainin...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1991-08-01 00:00:00
abstract::We have examined the pattern of expression of four different matrix metalloproteinases (MMPs), collagenase, stromelysin, 92 kD gelatinase, and 72 kD gelatinase, by primary and passaged cultures of rabbit corneal fibroblasts. Primary cultures of this cell type have previously been shown to reproduce the normal tissue r...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::The contractile vacuole of the chloromonadophycean alga Vacuolaria virescens is a permanent structure that possesses a specialized membrane: subunits of this membrane have a diameter of 21-24 nm and in places are arranged in a regular hexagonal pattern. The lateral walls of these subunits form regularly spaced bristle...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-06-01 00:00:00
abstract::p63 (also known as TP63) is a transcription factor of the p53 family, along with p73. Multiple isoforms of p63 have been discovered and these have diverse functions encompassing a wide array of cell biology. p63 isoforms are implicated in lineage specification, proliferative potential, differentiation, cell death and ...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.228015
更新日期:2020-09-11 00:00:00
abstract::SNAREs such as VAMP, SNAP-25 and syntaxin are essential for intracellular trafficking, but what are their exact molecular roles and how are their interactions with other proteins manifest? Capitalizing on the differential sensitivity of SNAREs to exogenous proteases, we quantified the selective removal of identified S...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00374
更新日期:2003-05-15 00:00:00
abstract::In neutrophils and other phagocytic cells, the small GTPase Rac is an essential regulator of a multi-component NADPH oxidase that produces high levels of superoxide, which kills invading pathogens. In many other cell types, Rac and newly discovered relatives of the neutrophil burst oxidase and its subunits have been f...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.00937
更新日期:2004-01-15 00:00:00
abstract::Drosophila bristle cells form enormous extensions that are supported by equally impressive scaffolds of modular, polarized and crosslinked actin filament bundles. As the cell matures and support is taken over by the secreted cuticle, the actin scaffold is completely removed. This removal begins during cell elongation ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-02-01 00:00:00
abstract::The production, transport, and disposition of material labelled with [3H]mannose were studied in microsurgically enucleated and control amoebae. Cells were injected with the precursor and samples were prepared for electron-microscope radioautography at intervals, up to 24 h later. Control cells showed heavy labelling ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1984-06-01 00:00:00
abstract::Myogenic differentiation in adult muscle is normally suppressed and can be activated by myogenic cues in a subset of activated satellite cells. The switch mechanism that turns myogenesis on and off is not defined. In the present study, we demonstrate that tissue inhibitor of metalloproteinase 3 (TIMP3), the endogenous...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.057620
更新日期:2010-09-01 00:00:00
abstract::The position of the nucleus is regulated in different developmental stages and cellular events. During polarization, the nucleus moves away from the future leading edge and this movement is required for proper cell migration. Nuclear movement requires the LINC complex components nesprin-2G and SUN2, which form transme...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.087049
更新日期:2012-03-01 00:00:00