Abstract:
:The production, transport, and disposition of material labelled with [3H]mannose were studied in microsurgically enucleated and control amoebae. Cells were injected with the precursor and samples were prepared for electron-microscope radioautography at intervals, up to 24 h later. Control cells showed heavy labelling of the rough endoplasmic reticulum and the Golgi apparatus at early intervals after injection. Later, labelling of groups of small vesicles increased, and the percentage of grains over the cell surface peaked 12 h after administration of the precursor. Two major changes were detected in enucleate amoebae. First, the kinetics of labelling of cell organelles with [3H]mannose were altered in the absence of the nucleus. The Golgi apparatus and cell surface both displayed maximal labelling at later intervals in enucleates, and the percentage of grains over the rough endoplasmic reticulum varied less with time in enucleated than in control cells. Second, the distribution of radioactivity was altered. A greater percentage of grains was associated with lysosomes in enucleates than in control cells. The change in the kinetics of labelling of the endoplasmic reticulum, Golgi apparatus and cell surface indicates that intracellular transport of surface material was slower in the absence of the nucleus. It is suggested that this is related to the decreased motility of enucleate cells.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Flickinger CJsubject
Has Abstractpub_date
1984-06-01 00:00:00pages
83-94eissn
0021-9533issn
1477-9137journal_volume
68pub_type
杂志文章abstract::Interactions between melanocytes and neighboring cells in the skin are important in regulating skin color in humans. We recently demonstrated that the less pigmented and thicker skin on the palms and soles is regulated by underlying fibroblasts in those areas, specifically via a secreted factor (DKK1) that modulates W...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.064774
更新日期:2010-09-15 00:00:00
abstract::Using a series of chimeric and truncated N-acetylglucosaminyltransferase I (NAGT I) molecules we have shown that part of the lumenal stalk region is both necessary and sufficient for kin recognition of mannosidase II and retention in the Golgi stack. The membrane-spanning domain was not required for retention, but rep...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::As a contribution to the understanding of the mechanism of the collective motion of the cell mass in the migrating pseudoplasmodium its motive force has been determined. The measurements in the present paper were made by an original method using centrifugal force. The maximum value of the motive force of a migrating p...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1982-08-01 00:00:00
abstract::Although the 11.5 kDa Zn(2+)-binding protein (ZnBP, parathymosin-alpha) possesses a functional bipartite nuclear localization signal it was found in most tissues in the cytoplasm. The cultivation of freshly isolated rat hepatocytes for 24 hours under standard conditions was associated with an almost complete transloca...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::Several cell motility processes including cytokinesis and cell locomotion are dependent on the interplay of the microtubule and actomyosin cytoskeletons. However, because such processes are essentially visual phenomena, interactions between the two cytoskeletal systems have been difficult to study quantitatively. To o...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00
abstract::Intracellular pools of the heterotrimeric G-protein α-subunit Gαi3 (encoded by GNAI3) have been shown to promote growth factor signaling, while at the same time inhibiting the activation of JNK and autophagic signaling following nutrient starvation. The precise molecular mechanisms linking Gαi3 to both stress and grow...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.241034
更新日期:2020-06-24 00:00:00
abstract::During mouse meiosis, the early prophase RAD51/DMC1 recombination protein sites, which are associated with the chromosome cores and which serve as markers for ongoing DNA-DNA interactions, are in ten-fold excess of the eventual reciprocal recombinant events. Most, if not all, of these early interactions are eliminated...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-04-15 00:00:00
abstract::The growth of two human diploid skin fibroblast cell lines, originally grown in medium supplemented with foetal bovine serum and later adapted to medium supplemented with newborn bovine, bovine calf or horse serum, has been studied. Prolonged generation times increased cell volumes and decreased plating efficiencies w...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1983-05-01 00:00:00
abstract::Re-epithelialization after skin wounding requires both migration and hyperproliferation of keratinocytes. Laminin-332 is deposited during migration over the provisional matrix. To investigate the function of the laminin-332 binding integrin alpha3beta1 in wound re-epithelialization, we generated Itga3flox/flox; K14-Cr...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.029108
更新日期:2009-01-15 00:00:00
abstract::Collective cellular migration within the epithelial layer impacts upon development, wound healing and cancer invasion, but remains poorly understood. Prevailing conceptual frameworks tend to focus on the isolated role of each particular underlying factor - taken one at a time or at most a few at a time - and thus migh...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.187922
更新日期:2016-09-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
abstract::We previously reported that, among the three connexins expressed in chick lens, overexpression of connexin (Cx) 45.6, not Cx43 or Cx56, stimulates lens cell differentiation; however, the underlying mechanism responsible for this effect is unclear. Here, we took advantage of naturally occurring loss-of-gap-junction fun...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.000935
更新日期:2007-10-15 00:00:00
abstract::Cellular senescence suppresses cancer by arresting cells at risk of malignant tumorigenesis. However, senescent cells also secrete molecules that can stimulate premalignant cells to proliferate and form tumors, suggesting the senescence response is antagonistically pleiotropic. We show that premalignant mammary epithe...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01635
更新日期:2005-02-01 00:00:00
abstract::The morphogenesis-related NDR kinase (MOR) pathway regulates morphogenesis in fungi. In spite of the high conservation of its components, impairing their functions results in highly divergent cellular responses depending on the fungal species. The reasons for such differences are unclear. Here we propose that the spec...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.107862
更新日期:2012-10-01 00:00:00
abstract::The mitochondrial influx and efflux of Ca(2+) play a relevant role in cytosolic and mitochondrial Ca(2+) homeostasis, and contribute to the regulation of mitochondrial functions in neurons. The mitochondrial Na(+)/Ca(2+) exchanger, which was first postulated in 1974, has been primarily investigated only from a functio...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.129668
更新日期:2013-12-15 00:00:00
abstract::Thrombospondin-1 (TSP-1) and the highly related protein thrombospondin-2 (TSP-2) are trimeric extracellular molecules that have complex roles in wound healing, angiogenesis and matrix organisation. At the cellular level, TSP-1 supports cell adhesion and migration by the organisation of fascin spike cytoskeletal struct...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract::Inactivation of Simian Virus 40 large T antigen, in cells immortalized with conditional mutants, leads to activation of p53 and apoptosis. We used the mRNA differential display method to identify genes differentially expressed during this process. We found that steady-state levels of mRNA for cytoplasmic actins decrea...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::By passing a suspension of polymorphonuclear leucocytes through a parallel-plate chamber their adhesiveness can be assessed by scoring the number of cells trapped on the lower plate, and the fluid shear stress can be defined for a given flow rate. Since the adhesiveness of the cell at the instant of collision must exc...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1984-08-01 00:00:00
abstract::The control of bull spermatozoon flagellar activity has been investigated using direct current injection into the cells through an impaling glass microelectrode. Negative current injection results in a decrease in the flagellar frequency. Flagellar frequencies can be decreased to zero with high negative currents. This...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-02-01 00:00:00
abstract::By an improved freeze-fracture device, a number of double replicas were obtained from the rod disk membranes of the carp retina for investigation of complementary, continuing, and transitional relationships between fracture faces. Some separate particles found along the smooth edge bordering on the adjacent interdisk ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
abstract::At the onset of development, the single cells of the eukaryotic micro-organism Dictyostelium discoideum secrete proteolytic activity which can be assayed using the insoluble substrate remazolbrilliant blue hide. The activity is not secreted by exponentially growing cells, but does appear extracellularly at the onset o...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-04-01 00:00:00
abstract::Centrioles organise centrosomes and cilia, and these organelles have an important role in many cell processes. In flies, the centriole protein Ana1 is required for the assembly of functional centrosomes and cilia. It has recently been shown that Cep135 (also known as Bld10) initially recruits Ana1 to newly formed cent...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.186460
更新日期:2016-07-01 00:00:00
abstract::Using a polyclonal antibody against purified zymogen granule membrane components from rat pancreas a cDNA coding for the 29 kDa protein (ZG29p) was identified by immunoscreening of a hormonally stimulated pancreas cDNA library. Western blot analysis suggests that ZG29p is a pancreas-specific protein and immunofluoresc...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::Natural killer (NK) cells eliminate abnormal cells through the release of cytolytic granule contents. In this process, NK cells must adhere to target cells through integrin-mediated adhesion, which is highly dependent on the generation of F-actin. Ena/VASP-like (EVL) is an actin regulatory protein previously shown to ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.230508
更新日期:2019-07-18 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-03-01 00:00:00
abstract::It has been claimed repeatedly that gamma-tubulin is exclusively localized at the spindle poles in mitotic animal cells, where it plays a role in microtubule nucleation. In addition to this localization, we have observed a gamma-tubulin-specific staining of the mitotic spindle in several animal cells (human, kangaroo ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::Our previous studies of PAWS1 (protein associated with SMAD1; also known as FAM83G) have suggested that this molecule has roles beyond BMP signalling. To investigate these roles, we have used CRISPR/Cas9 to generate PAWS1-knockout U2OS osteosarcoma cells. Here, we show that PAWS1 plays a role in the regulation of the ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.202390
更新日期:2018-01-10 00:00:00
abstract::Multinucleated giant cells, characteristic of granulomatous infections, originate from the fusion of macrophages. Using an antibody screening strategy we found that the scavenger receptor CD36 participates in macrophage fusion induced by the cytokines IL-4 and GM-CSF. Our results demonstrate that exposure of phosphati...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.037200
更新日期:2009-02-15 00:00:00
abstract::The adaptor protein Miranda plays a pivotal role in the asymmetric cell division of neuroblasts by asymmetrically segregating key differentiation factors. Miranda localization requires Myosin VI and Myosin II. The apical-then-basal localization pattern of Miranda detected in fixed tissue, and the localization defects ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.020024
更新日期:2008-05-01 00:00:00
abstract::hnRNP A1 (34 kDa) is an RNA binding protein consisting of two tandemly arranged RNA binding domains C-terminally linked to a glycine-rich auxiliary domain (2 x RBD-Gly). A1 belongs to the set of polypeptides that bind nascent hnRNA in the nucleus to form the so called hnRNP complexes. These complexes seem to be involv...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-02-01 00:00:00