IGF binding protein 3 exerts its ligand-independent action by antagonizing BMP in zebrafish embryos.

abstract：:DNA replication in eukaryotic cells initiates from multiple replication origins that are distributed throughout the genome. Coordinating the usage of these origins is crucial to ensure complete and timely replication of the entire genome precisely once in each cell cycle. Replication origins fire according to a cell-t...

journal_title：Journal of cell science

authors： Yekezare M,Gómez-González B,Diffley JF

更新日期：2013-03-15 00:00:00

abstract：:During the first few cleavages of the zebrafish embryo, daughter blastomeres are loosely associated immediately after furrow ingression, but then gradually cohere. Cohesion appears to be cadherin-dependent, as cadherin and beta-catenin are found at the membrane between cohering blastomeres, and blastomeres fail to coh...

journal_title：Journal of cell science

authors： Jesuthasan S

更新日期：1998-12-18 00:00:00

abstract：:A number of recent studies have highlighted the importance of autophagy and the ubiquitin-proteasome in the pathogenesis of muscle wasting in different types of inherited muscle disorders. Autophagy is crucial for the removal of dysfunctional organelles and protein aggregates, whereas the ubiquitin-proteasome is impor...

journal_title：Journal of cell science

authors： Sandri M,Coletto L,Grumati P,Bonaldo P

更新日期：2013-12-01 00:00:00

abstract：:Megalin is a receptor expressed by embryonic epithelia that mediates endocytosis of numerous ligands, including sonic hedgehog (Shh) and retinol, the precursor to retinoic acid (RA). The importance of Shh and RA signaling in neurodevelopment, combined with the fact that megalin-deficient mice show profound neurodevelo...

journal_title：Journal of cell science

authors： McCarthy RA,Argraves WS

更新日期：2003-03-15 00:00:00

abstract：:Rho GTPases and their downstream effectors regulate changes in the actin cytoskeleton that underlie cell motility and adhesion. They also participate, with RhoA, in the regulation of gene transcription by activating serum response factor (SRF)-mediated transcription from the serum response element (SRE). SRF-mediated ...

journal_title：Journal of cell science

authors： Xie Y,Tan EJ,Wee S,Manser E,Lim L,Koh CG

更新日期：2008-02-15 00:00:00

abstract：:Despite observations that the lacrimal gland has been identified as the principal source of dimeric immunoglobulin A (dIgA) in tears, the mechanism used by lacrimal gland acinar cells (LGACs) to transcytose dIgA produced by interstitial plasma cells is not well-characterized. This study identifies a transcytotic pathw...

journal_title：Journal of cell science

authors： Xu S,Edman M,Kothawala MS,Sun G,Chiang L,Mircheff A,Zhu L,Okamoto C,Hamm-Alvarez S

更新日期：2011-10-15 00:00:00

abstract：:The protein kinase inhibitor H-7 prevents the assembly of tight junctions in cultured Madin Darby Canine Kidney (MDCK) epithelial cells (Balda et al. (1991) J. Membr. Biol. 122, 193-202; Nigam et al. (1991) Biochem. Biophys. Res. Commun. 181, 548-553); however, its mechanism of action is unknown. To understand the bas...

journal_title：Journal of cell science

authors： Denisenko N,Burighel P,Citi S

更新日期：1994-04-01 00:00:00

abstract：:Eukaryotic gene expression can be viewed within a conceptual framework in which regulatory mechanisms are integrated at three hierarchical levels. The first is the sequence level, i.e. the linear organization of transcription units and regulatory sequences. Here, developmentally co-regulated genes seem to be organized...

journal_title：Journal of cell science

authors： van Driel R,Fransz PF,Verschure PJ

更新日期：2003-10-15 00:00:00

abstract：:The spindle checkpoint detects errors in kinetochore attachment to microtubules and delays anaphase if attachment is improper. The checkpoint is activated by attachment-sensitive components including Mad2 and certain phosphorylated proteins detected by the 3F3/2 antibody. We have studied Mad2 and 3F3/2 immunofluoresce...

journal_title：Journal of cell science

authors： Nicklas RB,Waters JC,Salmon ED,Ward SC

更新日期：2001-12-01 00:00:00

abstract：:EPHA2 receptor tyrosine kinase is overexpressed in several human cancer types and promotes malignancy. However, the mechanisms by which EPHA2 promotes tumor progression are not completely understood. Here we report that overexpression of a wild-type EPHA2, but not a signaling-defective cytoplasmic truncation mutant (D...

journal_title：Journal of cell science

authors： Fang WB,Ireton RC,Zhuang G,Takahashi T,Reynolds A,Chen J

更新日期：2008-02-01 00:00:00

abstract：:Start is the main decision point in the eukaryotic cell cycle at which cells commit to a new round of cell division. It involves the irreversible activation of a transcriptional programme through the inactivation of Start transcriptional repressors: the retinoblastoma family in mammals, or Whi5 and its recently identi...

journal_title：Journal of cell science

authors： Méndez E,Gomar-Alba M,Bañó MC,Mendoza M,Quilis I,Igual JC

更新日期：2020-12-21 00:00:00

abstract：:The end1 mutant belongs to a group of four vacuolar protein sorting mutants (class C vps) that lack a morphologically distinguishable and functional vacuole. These mutants share several other phenotypes, such as the inability to grow at 37 degrees C or on nonfermentable carbon sources. We show that, as in the case of ...

journal_title：Journal of cell science

authors： Dulić V,Riezman H

更新日期：1990-11-01 00:00:00

abstract：:Ligand-dependent nuclear import is crucial for the function of the androgen receptor (AR) in both health and disease. The unliganded AR is retained in the cytoplasm but, on binding 5alpha-dihydrotestosterone, it translocates into the nucleus and alters transcription of its target genes. Nuclear import of AR is mediate...

journal_title：Journal of cell science

authors： Cutress ML,Whitaker HC,Mills IG,Stewart M,Neal DE

更新日期：2008-04-01 00:00:00

abstract：:The molecular chaperone Hsp90 has been found ubiquitously as a predominantly cytoplasmic dimer. By interacting with cytoplasmic or nuclear proteins such as pp60v-src or steroid receptors, Hsp90 helps its targets to become competent for full biological activity. Mutational deletion analysis of some properties of chicke...

journal_title：Journal of cell science

authors： Meng X,Devin J,Sullivan WP,Toft D,Baulieu EE,Catelli MG

更新日期：1996-07-01 00:00:00

abstract：:The metabolism of PI(3,5)P2 is regulated by the PIKfyve, VAC14 and FIG4 complex, mutations in which are associated with hypopigmentation in mice. These pigmentation defects indicate a key, but as yet unexplored, physiological relevance of this complex in the biogenesis of melanosomes. Here, we show that PIKfyve activi...

journal_title：Journal of cell science

authors： Bissig C,Croisé P,Heiligenstein X,Hurbain I,Lenk GM,Kaufman E,Sannerud R,Annaert W,Meisler MH,Weisman LS,Raposo G,van Niel G

更新日期：2019-02-28 00:00:00

abstract：:ARVCF is a novel Armadillo repeat domain protein that is closely related to the catenin p120(ctn). Using new ARVCF monoclonal antibodies, we have found that ARVCF associates with E-cadherin and competes with p120 for interaction with the E-cadherin juxtamembrane domain. ARVCF also localized to the nucleus in some cell...

journal_title：Journal of cell science

authors： Mariner DJ,Wang J,Reynolds AB

更新日期：2000-04-01 00:00:00

abstract：:Neural progenitors in the vertebrate nervous system are fully polarized epithelial cells, with intercellular junctions at the apical region. These progenitor cells remain within the neuroepithelium throughout neurogenesis, and will ultimately give rise to all the neurons in the mature nervous system. We have addressed...

journal_title：Journal of cell science

authors： Afonso C,Henrique D

更新日期：2006-10-15 00:00:00

abstract：:The nature, kinetics and cofactor requirements of leukocyte adhesion to cultured vascular cells have been investigated in vitro, using a model system in which leukocyte suspensions are in continuous motion over the cultured cells. Adhesion is assessed by histological examination or by using 51Cr-labelled leukocytes. L...

journal_title：Journal of cell science

authors： Beesley JE,Pearson JD,Carleton JS,Hutchings A,Gordon JL

更新日期：1978-10-01 00:00:00

abstract：:Endosomal sorting of internalized cell surface receptors to the lysosomal pathway plays a crucial role in the control of cell signaling and function. Here we report the identification of GABA(A) receptor interacting factor-1 (GRIF1), a recently discovered protein of unknown function, as a new regulator of endosome-to-...

journal_title：Journal of cell science

authors： Kirk E,Chin LS,Li L

更新日期：2006-11-15 00:00:00

abstract：:Although the kinase CHK1 is a key player in the DNA damage response (DDR), several studies have recently provided evidence of DDR-independent roles of CHK1, in particular following phosphorylation of its S280 residue. Here, we demonstrate that CHK1 S280 phosphorylation is cell cycle-dependent and peaks during mitosis....

journal_title：Journal of cell science

authors： Adam K,Cartel M,Lambert M,David L,Yuan L,Besson A,Mayeux P,Manenti S,Didier C

更新日期：2018-08-10 00:00:00

abstract：:Polarization of the motile cell is associated with the formation of a distinct plasma membrane domain, the pseudopod, whose stabilization determines the directionality of cell movement. The rapid movement of cells over a substrate requires that an essential aspect of cell motility must be the supply of the necessary m...

journal_title：Journal of cell science

authors： Nabi IR

更新日期：1999-06-01 00:00:00

abstract：:Besides regulating glucose levels, insulin has been reported to participate actively in many other functions, including modulating inflammatory reactions. In this study we investigated how topical insulin application would affect the diabetic wound healing process. We found that the excessive expression of insulin-deg...

journal_title：Journal of cell science

authors： Yang P,Wang X,Wang D,Shi Y,Zhang M,Yu T,Liu D,Gao M,Zhang X,Liu Y

更新日期：2020-10-12 00:00:00

abstract：:An essential step in mammalian fertilisation is the sperm acrosome reaction (AR) - exocytosis of a single large vesicle (the acrosome) that surrounds the nucleus at the apical sperm head. The acrosomal and plasma membranes fuse, resulting in both the release of factors that might facilitate penetration of the zona pel...

journal_title：Journal of cell science

authors： Harper CV,Cummerson JA,White MR,Publicover SJ,Johnson PM

更新日期：2008-07-01 00:00:00

abstract：:Serial sections of mitotic spindles of the marine cryptophycean alga, Cryptomonas, were analysed to determine what types of microtubules they contained and which of these microtubules came close enough to each other (50 nm or less) for the commonly described crossbridging to be possible. Interpolar microtubules were r...

journal_title：Journal of cell science

authors： Oakley BR,Heath IB

更新日期：1978-06-01 00:00:00

abstract：:The LEM domain (for lamina-associated polypeptide, emerin, MAN1 domain) defines a group of nuclear proteins that bind chromatin through interaction of the LEM motif with the conserved DNA crosslinking protein, barrier-to-autointegration factor (BAF). Here, we describe a LEM protein annotated in databases as 'Ankyrin r...

journal_title：Journal of cell science

authors： Brachner A,Braun J,Ghodgaonkar M,Castor D,Zlopasa L,Ehrlich V,Jiricny J,Gotzmann J,Knasmüller S,Foisner R

更新日期：2012-02-15 00:00:00

abstract：:Multinucleated muscle fibres arise by fusion of precursor cells called myoblasts. We previously showed that CKIP-1 ectopic expression in C2C12 myoblasts increased cell fusion. In this work, we report that CKIP-1 depletion drastically impairs C2C12 myoblast fusion in vitro and in vivo during zebrafish muscle developmen...

journal_title：Journal of cell science

authors： Baas D,Caussanel-Boude S,Guiraud A,Calhabeu F,Delaune E,Pilot F,Chopin E,Machuca-Gayet I,Vernay A,Bertrand S,Rual JF,Jurdic P,Hill DE,Vidal M,Schaeffer L,Goillot E

更新日期：2012-08-15 00:00:00

abstract：:The complex formed by Rad9, Rad1 and Hus1 (9-1-1) protects against genomic instability by activating DNA damage checkpoint and DNA damage repair pathways, mainly in response to replication fork collapse and UV lesions. Here we compare the role of Rad9A (also known as Rad9) with the human paralogue Rad9B. Unlike Rad9A,...

journal_title：Journal of cell science

authors： Pérez-Castro AJ,Freire R

更新日期：2012-03-01 00:00:00

abstract：:Dystrophic forms of epidermolysis bullosa (DEB) are associated with mutations in the type VII collagen gene (Col7a1) which encodes the major component of anchoring fibrils. To develop a DEB animal model, type VII collagen deficient mice were generated by targeted homologous recombination. The targeting vector replaced...

journal_title：Journal of cell science

authors： Heinonen S,Männikkö M,Klement JF,Whitaker-Menezes D,Murphy GF,Uitto J

更新日期：1999-11-01 00:00:00

abstract：:The model organism Neurospora crassa undergoes programmed cell death when exposed to staurosporine. Here, we show that staurosporine causes defined changes in cytosolic free Ca(2+) ([Ca(2+)]c) dynamics and a distinct Ca(2+) signature that involves Ca(2+) influx from the external medium and internal Ca(2+) stores. We i...

journal_title：Journal of cell science

authors： Gonçalves AP,Cordeiro JM,Monteiro J,Muñoz A,Correia-de-Sá P,Read ND,Videira A

更新日期：2014-09-01 00:00:00

abstract：:The mitotic spindle has long been recognized to play an essential role in determining the position of the cleavage furrow during cell division, however little is known about the mechanisms involved in this process. One attractive hypothesis is that signals from the spindle may function to induce reorganization of cort...

journal_title：Journal of cell science

authors： Fishkind DJ,Silverman JD,Wang YL

更新日期：1996-08-01 00:00:00